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  • 1. PCR : POLYMERASE CHAIN REACTION
  • 2.
  • 3.
  • 4. INTRODUCTION
    PCR is one of the most important technique and it is also a key procedure in numerous basic studies involving DNA molecules.
    All methods for PCR are based on the concept of DNA strand complementarity .
  • 5. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
  • 6. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
    GPC3 is a member of the glypican family of glycosylphosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans.
  • 7. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
    GPC3 is highly expressed in HCC cells and tissues. It is thought that GPC3 joins a multiprotein complex, which is composed of the ligand, receptor, GPC3, and probably other proteins.
  • 8. A NEW ROLE OF GLYPICAN-3 IN HEPATOCELULAR CARCINOMA
    This is the first study to report that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.
  • 9. OBSERVATION
    The PCR is an important, useful and fast technique that could help find an appropriate diagnosis before symptom expression.
  • 10. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
  • 11. The real-time PCR assay delivered results within two hours, whereas culture results were examined after 48 hours.
    CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
  • 12. Although PCR has a high risk of false positivity, This may demonstrate a better detection sensitivity in the PCR assay.
    CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
  • 13. CERTAIN LABORATORY TECHNIQUE ALLOWS RAPID DETECTION OF EYE PATHOGENS
    Real-time PCR can accurately and simultaneously detect bacterial and fungal pathogens in a speedy fashion.
  • 14. OBSERVATION
    The PCR technique should be a requirement for eye pathogens diagnosis, because it leads to a diagnosis and therefore more specific treatments.
  • 15. MEDICAL UTILITY
  • 16. MEDICAL UTILITY
    Polymorphismdiagnostic .
    Characterizationfortransplants .
    Establishmutation
  • 17. DNA clonation
    mutation diagnostic
    detection of tumor cells
    MEDICAL UTILITY
  • 18. prenatal diagnosis disease
    genetic screening
    detection of infectious microorganisms ,
    MEDICAL UTILITY
  • 19. BIBLIOGRAPHY
    MARTINEZ SANCHEZ,Lina María, Biología Molecular, 5 edición, Medellín, UPB, Fac de Medicina 2009, 139p-147p.
     
    http://www.sciencedaily.com/releases/2010/05/100510161300.htm
     
    http://www.sciencedaily.com/releases/2010/08/100802091207.htm
  • 20. PICTURE
    Mejor es callar y que sospechen de tu poca sabiduría que hablar y eliminar cualquier duda sobre ello.
    Abraham Lincon