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Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
Investigation in dengue
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  • Thank you .it's been very useful information.
    In your experience ;what is the probability of a false negative Ig M dengue test ?(primary infection)
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  1. LABORATORY INVESTIGATION IN DENGUE INFECTION Dr Lai Jun Yuan Pegawai Perubatan Jabatan Patologi Hospital Batu Pahat
  2.  DISEASE MONITORING TESTS  DIAGNOSTIC TESTS
  3. 1. DENGUE SEROLOGY TESTS • Haemagglutination Inhibition Test • Dengue IgM Test • Indirect IgG ELISA Test • Dengue Rapid Tests 2. VIRUS ISOLATION 3. POLYMERASE CHAIN REACTION (PCR) 4. NON STRUCTURAL PROTEIN – 1 (NS1 Antigen)
  4. HAEMAGGLUTINATION INHIBITION TESTS • Gold Standard for serological diagnosis • Labour intensive & requires paired samples for interpretation • Differentiate between 1° & 2 ° dengue infection • Used mainly for research purpose
  5. DENGUE IgM TEST • Widely used serological test. • Significantly higher in primary infections
  6. INDIRECT IgG ELISA TEST • Detected after 7 days of onset of fever. • Recommended if dengue IgM is still negative after day 7 in secondary dengue.
  7. DENGUE RAPID TESTS • Qualitative detection of dengue IgM & IgG • Possible of False Positive • Need to confirm by dengue IgM ELISA tests
  8. VIRUS ISOLATION • Most definitive test for dengue infection • Performed in Lab equipped with tissue & virus isolation facilities • Time consuming (2 weeks) • Expensive
  9. POLYMERASE CHAIN REACTION (PCR) • Diagnosis of dengue infection in the early phase (< 5 days of illness)
  10. POLYMERASE CHAIN REACTION (PCR)
  11. NON STRUCTURAL PROTEIN – 1 (NS1 Antigen) • This antigen is present in high concentrations in the sera of dengue infected patients during the early phase of the disease
  12.  Dengue Rapid Test  Possible of false positive  Confirmation with ELISA  Please call the lab prior to sending any samples  TAT – 1hour
  13.  Dengue IgM  ELISA method  Done in batches  TAT – 2 to 3 days
  14.  Dengue IgG (Special request)  ELISA method  TAT – 2 to 3 days
  15.  NS1 Antigen  Rapid NS1  Please call the lab prior to sending any samples  TAT – 1hour
  16. • NS1 Antigen (ELISA method)  ELISA method  Done in batches (twice a week)  TAT – 2 to 3 days
  17. Please write down the type of Tests: 1) Dengue IgM 2) Dengue IgG 3) Rapid IgM/IgG 4) NS1 Antigen 5) Rapid NS1
  18. • For NS1 Antigen Test only • The form must be completely filled  Patient details  Date of onset  Clinical Finding
  19. Dengue investigation is very costly Please plan carefully before you send any investigation for dengue Investigation must base on clinical presentation of the patient
  20. • Clinical Practice Guildlines “Management of Dengue Infection in Adults” 2nd Edition by MOH

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