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  • 07/23/12
  • 07/23/12

Transcription and translation Transcription and translation Presentation Transcript

  • Molecular Genetics - From DNA to Trait
  • How Are Different Types of Cells Created and Maintained?By differential gene expression.The same genetic information is in all 100 trillion cells ofany one person. Different cells use the same blueprint indifferent ways.How?In essence, the control of geneexpression occurs by regulatingthe flow of information fromDNA to protein.
  • The “Central Dogma” of Molecular GeneticsTranscription TranslationDNA RNA Protein Trait RNA processing
  • Transcription is a Key Step in Gene Expression Transcription makes an RNA copy of DNA.
  • RNARNA is a nucleic acid polymer that uses a slightly different sugar than DNAand the base uracil (U) in place of thymine (T).
  • RNA Is Largely Single-Stranded
  • There are Different RNAs with Distinct FunctionsRecently, a new class of RNA, microRNA, has been shown toregulate gene expression.
  • Transcription RNA polymerase acts hereThe enzyme RNA polymerase opens the DNA strands and synthesizes an RNAcomplementary to only one of the DNA strands.
  • Transcription A geneThe decision to transcribe a gene is the most important step in the control of geneexpression.Transcription starts and stops at distinct sites at the ends of a gene. Animation
  • Eukaryotic Genes are SegmentedGenes are made of parts represented in the mRNA (exons) and parts that aretranscribed but not present in the mRNA (introns).Introns are removed from the primary transcript and exons are spliced together tomake mRNA.In some genes more than 90% of the pre-mRNA is destroyed, never to appear inthe mRNA. Animation
  • Alternative Splicing – More Bang for the BuckThis has the consequence that the count of our genes (~20,000) seriouslyunderestimates the count of our different proteins.
  • The Genetic Language Uses 4 Letters Written Into 3-Letter Words
  • Amino Acids – What the GeneticCode Specifies Two examples There are 20 different amino acids
  • What Translation Accomplishes The sequence of amino acids determines the structure, and therefore the function, of a protein.In translation, information present in the mRNA is read by the ribosome tosynthesize a polypeptide.
  • Translation Is ComplicatedManyantibioticsblock steps intranslationwithinbacterial cells. Translation requires: ribosomes mRNA tRNA amino acids
  • tRNA Is An Adpator That Couples Codons and Amino Acids
  • The Genetic Code is Biology’s Rosetta Stone These are the words of the genetic language.
  • Ribosomes are ComplicatedProtein Synthesizing Machines
  • Translation Is a Cyclic, Multistep ProcessTranslation Animation
  • Basic Genetic Mechanisms are UniversalThe storage of genetic information in DNA, theuse of an RNA intermediate that is read in threeletter words, and the mechanism of proteinsynthesis are essentially the same in allorganisms. A tobacco plant expressing the firefly luciferaseAmong other things, this means cancer can be gene.studied productively in flies or yeast.It also means that human genes can beexpressed in a plant or mouse genes in a yeast.