Chapters 1 3 basic lab techniques

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Chapters 1 3 basic lab techniques

  1. 1. BASIC LAB TECHNIQUES MODULE 1 CHAPTERS 1-3 Modern Biotechnology industry Business of biotechnology Life cycle of pharma products
  2. 2. DEFINITION OFBIOTECHNOLOGY the manipulation (as through genetic engineering) of living organisms or their components to produce useful usually commercial products (as pest resistant crops, new bacterial strains, or novel pharmaceuticals); FROM THE FREE MARRIAM WEBSTER DICTIONARY Chapter 1
  3. 3. THE EUROPEAN COMMISION VS.FDA "The application of science and technology to living organisms, as well as parts, products and models thereof, to alter living or non-living materials for the production of knowledge, goods and services.“ “The application of biological systems and organisms to technical and industrial processes” chapter 1
  4. 4. Brief Review of Molecular biology Biotechnology is used to manipulate DNA DNA instructs the cells how to make proteins DNA is located in the chromosomes and contain genes Genes express themselves by producing proteins Some proteins are structural others are regulatory When cells express specific genes and not others we call them differentiated. Embryonic cells can express all genes therefore they are undifferentiated
  5. 5. Question? DNA resides in the nucleus,proteins are produced in theribosomes (cytoplasmicorganelles), how does theinformation move from thenucleus to the ribosomes?
  6. 6. answerm-RNA
  7. 7. Techniques in biotechnology To manipulate cells we use: Genetic engineering Recombinant DNA Transformation Transfection Fermentation in bioreactors Use vectors and plasmids In vitro technology like cell and tissue culture
  8. 8. The Genome project Genomics ProteomicsBioinformatics
  9. 9. Applications Biopharma – products manufactured using genetically modified organisms e.g insulin Use of bacteria, mammalian and plant cells pros and cons? In vitro and in vivo systems. Examples? Gene therapy Bioagriculture Regenerative medicine and tissue engineering using stem cells Biofues forensics
  10. 10. The business of biotechnology Research and development Production QC/QA Regulatory affairs chapter 2

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