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Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
Primer design
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Primer design

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primer design ,help youshelf

primer design ,help youshelf

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  1. 1. PCR primer design• Local software Primer premier5, Oligo 6.1• Web-based design Primer3 (Whitehead Institute/MIT Center for Genome Research)
  2. Primer premier5
  3. Principles for primer design• Base composition• Length• Repeated and self-complementary seq.• Cross dimer• Tm• 3’ Termini• Priming sites (From Molecular cloning 3, 8.14)

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