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By: Stephen Feagles
What is PCR? the automated process that allows a specific DNA chain also called primers to be replicated over and over ag...
Who Invented it? Dr. Kary Banks Mullis born on December 28, 1944 Ph.D. degree in biochemistry from the University of  C...
Thermocycler This machines purpose is to alter the temperature so that the primers can be heated and cooled to perform th...
Steps of PCR1. Denature2. Annealing3. Extension
Denature Heating up DNA Splitting into two strands 94 degrees Celsius
Annealing Cooling Down Allowing to be bonded 54 degrees Celsius
Extension Taq Enzyme creating copy of original DNA Two new double stranded DNA formed 72 degrees Celsius
Process of PCR (video) http://www.youtube.com/watch?v=eEcy9k_KsDI
PCR Importance Creates more DNA    More tests run on specific DNA    More DNA can be tested by different people Forens...
Bibliography http://vadlo.com/Research_Cartoons/ http://www.youtube.com/watch?v=eEcy9k_KsDI http://flickrhivemind.net/T...
Polymerase chain reaction
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Polymerase chain reaction

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Transcript of "Polymerase chain reaction"

  1. 1. By: Stephen Feagles
  2. 2. What is PCR? the automated process that allows a specific DNA chain also called primers to be replicated over and over again to produce more copies of that DNA
  3. 3. Who Invented it? Dr. Kary Banks Mullis born on December 28, 1944 Ph.D. degree in biochemistry from the University of California, Berkeley Won Nobel Prize in Chemistry for PCR
  4. 4. Thermocycler This machines purpose is to alter the temperature so that the primers can be heated and cooled to perform the processes correctly
  5. 5. Steps of PCR1. Denature2. Annealing3. Extension
  6. 6. Denature Heating up DNA Splitting into two strands 94 degrees Celsius
  7. 7. Annealing Cooling Down Allowing to be bonded 54 degrees Celsius
  8. 8. Extension Taq Enzyme creating copy of original DNA Two new double stranded DNA formed 72 degrees Celsius
  9. 9. Process of PCR (video) http://www.youtube.com/watch?v=eEcy9k_KsDI
  10. 10. PCR Importance Creates more DNA  More tests run on specific DNA  More DNA can be tested by different people Forensics  Allows small amount of DNA to be made into a lot Research  Mutated DNA can be reproduced and be tested different ways
  11. 11. Bibliography http://vadlo.com/Research_Cartoons/ http://www.youtube.com/watch?v=eEcy9k_KsDI http://flickrhivemind.net/Tags/lab,pcr/Interesting http://www.sivb.org/InVitroReport/IVRs/38- 1/karyMullis.asp http://www.btci.org/k12/bft/PCR/pcr_geneticscreenin g.html
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