Polymerase chain reaction
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Polymerase chain reaction

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    Polymerase chain reaction Polymerase chain reaction Presentation Transcript

    • By: Stephen Feagles
    • What is PCR? the automated process that allows a specific DNA chain also called primers to be replicated over and over again to produce more copies of that DNA
    • Who Invented it? Dr. Kary Banks Mullis born on December 28, 1944 Ph.D. degree in biochemistry from the University of California, Berkeley Won Nobel Prize in Chemistry for PCR
    • Thermocycler This machines purpose is to alter the temperature so that the primers can be heated and cooled to perform the processes correctly
    • Steps of PCR1. Denature2. Annealing3. Extension
    • Denature Heating up DNA Splitting into two strands 94 degrees Celsius
    • Annealing Cooling Down Allowing to be bonded 54 degrees Celsius
    • Extension Taq Enzyme creating copy of original DNA Two new double stranded DNA formed 72 degrees Celsius
    • Process of PCR (video) http://www.youtube.com/watch?v=eEcy9k_KsDI
    • PCR Importance Creates more DNA  More tests run on specific DNA  More DNA can be tested by different people Forensics  Allows small amount of DNA to be made into a lot Research  Mutated DNA can be reproduced and be tested different ways
    • Bibliography http://vadlo.com/Research_Cartoons/ http://www.youtube.com/watch?v=eEcy9k_KsDI http://flickrhivemind.net/Tags/lab,pcr/Interesting http://www.sivb.org/InVitroReport/IVRs/38- 1/karyMullis.asp http://www.btci.org/k12/bft/PCR/pcr_geneticscreenin g.html