Nas Enzymes Lesson 2
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Nas Enzymes Lesson 2

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  • Protein in nature
  • Protein in nature

Nas Enzymes Lesson 2 Nas Enzymes Lesson 2 Presentation Transcript

  • Lesson Review
    Definition of Enzymes
    Properties of Enzymes
    • Speeds up chemical reaction
    • Required in small amounts
    • Highly specific in action
    • Work best at their optimum temperature and pH
    • May need co-enzymes for activity
  • Lesson Objectives
    1. Review Enzyme Definition & Enzyme Action
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
    NEW
  • Lesson Objectives
    1. Review Enzyme Definition & Enzyme Action
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
  • Definition of Enzymes
    Enzymes are biological catalysts that alters/speed upthe rate of a chemical reaction and remains chemically unchanged at the end of reaction.
  • Definition of Enzymes
    made of proteins
    Enzymes are biological catalysts that alters/speed upthe rate of a chemical reaction and remains chemically unchanged at the end of reaction.
    ^
  • Enzyme in action
    Substrates
    Enzyme
    Enzyme-substrate complex
    +
    products
    +
    Active sites
  • Lesson Objectives
    1. Review Enzyme Definition
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
  • Lesson Objectives
    1. Review Enzyme Definition
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
  • Properties of an enzyme
    Speeds up chemical reaction
    Required in small amounts
    Highly specific in action
    Work best at their optimum temperature and pH
    May need co-enzymes for activity
  • Properties of an enzyme
    Speeds up chemical reaction
    Required in small amounts
    Highly specific in action
    Work best at their optimum temperature and pH
    May need co-enzymes for activity
  • Enzymes are Highly Specific In Action
    Each chemical reaction in a cell is catalyzed by unique enzyme(s).
    Sucrose  Sucrase
    Lipid  Lipase
  • Why are enzymes highly specific in action?
  • We explain enzyme specificity via the Lock & Key Hypothesis
  • Lock & Key Hypothesis
    Presence of Active sites (depressions)
    Substrates fit into active site of enzyme
    Enzyme = lock; Substrate = key
    shape of substrate complementary to shape of enzyme.
  • Properties of an enzyme
    Speeds up chemical reaction
    Required in small amounts
    Highly specific in action
    Work best at their optimum temperature (practical) and pH
    May need co-enzymes for activity (FYIO)
    Both properties and factors affecting enzymes
  • Lesson Objectives
    1. Review Enzyme Definition
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
  • Lesson Objectives
    1. Review Enzyme Definition
    2. Properties of Enzymes
    3. Factors affecting Enzyme Activity
  • What are the factors affecting enzyme activity?
  • Factors Affecting Enzyme Activity
    Temperature
    pH
    Enzyme Concentration
    Substrate Concentration
    Inhibitors (FYIO)
  • How is enzyme activity affected by temperature?
    http://www.kscience.co.uk/animations/model.swf
    http://www.kscience.co.uk/animations/model.swf
  • Factors Affecting Enzyme Activity
    Temperature
    pH
    Enzyme Concentration
    Substrate Concentration
    Inhibitors (FYIO)
  • How is enzyme activity affected by pH?
  • How is enzyme activity affected by pH?
    http://www.kscience.co.uk/animations/model.swf
    http://www.kscience.co.uk/animations/model.swf
  • Factors Affecting Enzyme Activity
    Temperature
    pH
    Enzyme Concentration
    Substrate Concentration
    Inhibitors (FYIO)
  • Toothpickase activity
    Directions:
    Get into pairs.
    Each pair will receive 70 toothpicks and a stopwatch.
    Use 35 toothpicks for part I and 35 toothpicks for part II.
    1 person will be the ‘breaker’ the other the timer.
    Do only pg 1 & 2 in class…
  • What can you deduce from the ‘Toothpickase’ activity?
    If enzyme concentration increases,
    number of products form increases.
  • Questions to think about
    What if you only had 10 toothpicks for the activity?
    What happens to the rate of enzymatic reactions at very cold and very high temperatures?
    What are some of the properties of the enzymes that are found in the human stomach?
  • Summary
    Enzyme definition
    Enzymes are biological catalysts made of protein that alters/speed upthe rate of a chemical reaction and remains chemically unchanged at the end of reaction.
    Properties of Enzymes
    Speeds up
    Small/minute amounts
    Highly specific in action
    Work best at their optimum temperature and pH
    May need co-enzymes for activity
    Factors affecting Enzyme Activity
    -Temperature - pH
    - Substrate Concentration - Enzyme Concentration
  • Homework
    Complete ‘Toothpickase’ WS
    3. Do workbook Ex 3.2 ‘Enzymes’ pg 29-32 by tomorrow (fri 12th Mar 10).
    Explore ‘Factors affecting enzyme rate of reaction’ website http://www.kscience.co.uk/animations/model.swf
    Read up textbook pg 77-85 to prepare for short quiz next lesson to prepare for quiz when school re-opens.
  • Next Lesson
    Tips for experiment on
    “Effects of temperature on amylase (enzyme) activity”