Establishment of hairy root culture with genetic transformation
PROTEGES-Kartikey Vikram Singh
MENTOR-Santosh Kr. Mishra
Recently there has been a perceptible revival of
interest in the use of medicinal plants
throughout the world culminating into a
manifold increase of medicinal plant based
At present there are about 130 clinically useful
prescription drugs of known chemical
structures, solely derived from higher plants
and used in modern system of medicines.
In view of the spectacular increase in the
demand of plant based compounds, the
concerned medicinal plants have been
indiscriminately exploited leading to scarcity
or extinction of many valuable plant species.
This project deals with the production of highvalue secondary metabolites including
pharmaceuticals and food additives through
root cultures and transgenic roots obtained
through biotechnological means.
Recent developments in transgenic research
have opened up the possibility of the metabolic
engineering of biosynthetic pathways to
produce high-value secondary metabolites.
It is the culture produced after the infection of
explants or cultures by the gram negative soil
bacterium Agrobacterium rhizogenes.
This processes take advantage of the naturally
occurring hairy root disease in Dicotyledons.
molecules exuded by wounded plant cells and
becomes attached to it.
The bacteria contain the Root inducing plasmid
(Ri-plasmid) The bacteria genetically transfer
part of the Ri-plasmid called the transfer DNA
(T-DNA) to the plant genome.
where it gets expressed and make the plant cell
to proliferate by increasing the rate of cell
division (cytokine expression) and cell
elongation ( auxin expression) to produce the
Produce the opines which is a type of unusual
amino acids (octopine, agropine, nopaline,
mannopine, and cucumopine) which is used by
the bacterium as a carbon, nitrogen and energy
The hairy root system is genetically and
High production of secondary metabolites.
The culture can grow under phyto-hormonefree conditions.
The culture shows fast growth which reduces
the culture time and easy the handling
The main aim of this project is to synthesize secondary
metabolites from hairy root culture of medicinal plants
this process will comprise of following stages:
Selecting a plant species appropriate for the desired
Through Micropropagation obtaining an infection free
By means of genetic transformation induce the hairy
Obtaining the product and analysing the final product
Observing effect of different chemicals on the culture.
Literature and Review
Micro propagation of the Selected
Optimisation of different parameters
effect of different types of chemical
factor on hairy root culture
Analysis of secondary metabolites
produced by hairy roots
To grow the culture of A. rhizogenes we can
prepare media either LB or YEB.
To grow the hairy root we are preparing MS
media and enzyme supplement stock solutions.
Establishment of culture room is in progress.
Selection of the plant species for micro
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Biotechnology advances 20 (2002) 101 – 153; Plant cell
cultures: Chemical factories of secondary metabolites.
Suchitra Banerjee, Sailendra Singh, Laiq Ur Rahman;
Biotechnology advances 30 (2012) 461–468;
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