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Embryo Transfer

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  • 1. Embryo Transfer in Beef Cattle Ms. Bailes AGSC 336 – Advanced Animal Science
  • 2.  
  • 3. Objectives
    • Define embryo transfer
    • Explain the steps of embryo transfer
    • List the advantages of embryo transfer
    • List the disadvantages of embryo transfer
  • 4. What is Embryo Transfer? Collecting embryos from a superovulated donor cow... … and transferring the embryos to synchronized recipients to complete the gestation period.
  • 5. The Donor Cow
    • Top quality female, free of reproductive abnormalities or genetic defects, proven maternal qualities.
    • Personal goals/preferences play large part in donor selection.
    • Marketability of the donors calves.
  • 6. Superovulation of Donor Day 0 to Day 4
    • Follicle Stimulating Hormones
    • (Known as FSH)
    • Injections 2x daily, 4 days
    • (Day 0 starts 8-14 days following estrus cycle.)
  • 7. Superovulation--Day 3
    • Prostaglandin injection causing Corpus Luteum regression to bring on estrus.
    Result: Multiple Folicles Result: Multiple Folicles
  • 8. Superovulation--Day 5
    • Onset of estrus
  • 9. A.I. of Superovulated Donor
    • Artificially Inseminate when in standing estrus, usually 5 days after superovulation.
    • Usually multiple inseminations at 12, 24, 36 hrs. after onset of estrus.
    • Preferably, more than one straw of high quality semen.
  • 10. Embryo Recovery from Donor
    • Approximately 7 days after breeding.
    • Easy, takes less than 30 minutes.
    • Insert foley catheter with inflatable balloon into donor’s uterus.
    • Flushing solution (saline) is introduced into each uterine horn.
  • 11. Recovery of Embryos
    • Solution filled uterine horn is gently massaged.
    • Fluid containing the embryos is drawn back out and collected though a filter into a holding cylinder.
  • 12. Embryo Processing
    • After 20-30 minutes embryos located with stereoscopic microscope.
    • Embryos are washed and transferred to holding medium. (Procedure generally repeated 3 times.)
    • Embryos are evaluated for state of development and quality.
    • Initially classified: “good” or “bad.” “Good” embryos are further classified.
  • 13. The Recipient
    • Reproductively sound, good mothering ability.
    • Good overall health, nutrition.
    • Must be synchronized to receive the embryo.
  • 14. Synchronization Protocol--Day 0
    • Insert intravaginal CIDR.
    • Administer 2 cc combo Estradiol Benzoate and Progesterone.
  • 15. Day 7
    • Remove CIDR
    • Administer 5cc Prostaglandin
  • 16. Day 8
    • Final Injection:
    • 1 cc Estradiol Benzoate
  • 17. Day 8 through Day 10
    • Day 8 pm through Day 10am:
    • Observe for onset of estrus.
  • 18. Day 16--Transfer Embryo
    • Transfer Embryo into synchronized recipient who has come into estrus .
    • Procedure is simple,
    • much like A.I.
  • 19. Activity
    • Each card has a step in the embryo transfer process.
    • Put yourselves in the proper order of embryo transfer and then explain the process.
  • 20. Advantages of E.T.
    • Increased number of calves of genetically
    • superior cows.
    • Increased marketing opportunities--
    • offspring AND embryos.
    • Ease of Import/Export.
    • Embyros can be stored indefinitely.
  • 21. Disadvantages of E.T.
    • Increased expenses and higher break-even costs for
    • calves.
    • Estrus detection required.
    • Synchronization of recipient with donor.
    • Specialized equipment and trained personnel.
    • More expensive and time consuming than
    • traditional reproductive methods.
  • 22. In Summary…
    • E.T. is not for every herd.
    • Each female born has thousands of potential eggs.
    • Complete the crossword puzzle which is a summary/review of what we learned today.
  • 23.