Metabolomics. Strategic approach for GC amenable compounds

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Metabolomics is the newest hype in the 'omics' family. Although defined as highly important to recent biological research, the analytical science applied is far from acceptable for the majority of publications that appear nowadays. Take a look at our approach to tackle the metabolomics issue, which remains a new name for an old science.

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Metabolomics. Strategic approach for GC amenable compounds

  1. 1. METABOLOMICS A strategic approach for GC amenable compounds Interscience Expert Center www.is-x.be
  2. 2. Analysis of small-molecule metabolites formed by the cell in association with its metabolism. “
  3. 3. Accurate reflection of changes in cell functions taking genotype and phenotype into account.
  4. 4. Bacteria Food quality assessment Environmental remediation Antibiotics discovery
  5. 5. Plants Stress assessment studies Identification of gene function Cultivar differentiation
  6. 6. Mammals Health status assessment Prediction of clinical outcomes Patient classification
  7. 7. <ul><ul><li>Glycomics </li></ul></ul><ul><ul><li>Lipidomics </li></ul></ul><ul><ul><li>Flavouromics </li></ul></ul><ul><ul><li>Environmental metabolomics </li></ul></ul>Emerging spin-offs
  8. 8. Renewed hype for an ‘old science’ Classical biochemical concepts Classical analytical techniques
  9. 9. <ul><ul><li>Metabolite screening </li></ul></ul>“ Generic approach to detect & identify all metabolites in a given cell or organism”
  10. 10. <ul><ul><li>Metabolite fingerprinting </li></ul></ul>“ Identify and compare chromatographic patterns in response to change”
  11. 11. <ul><ul><li>Metabolite profiling </li></ul></ul>“ Detect & quantify a class of compounds in a given cell or organism”
  12. 12. <ul><ul><li>Metabolite target analysis </li></ul></ul>“ Detect & quantify a limited and specific set of compounds in a given cell or organism”
  13. 13. Approximately 85% of the articles published in ‘Metabolomics’ apply non-target screening. Carolyn Honour, Editorial Director ‘Metabolomics’ (Springer)
  14. 14. Unfortunately, the non-target screening approach suffers from some serious drawbacks.
  15. 15. <ul><ul><li>Extremely complex matrices </li></ul></ul><ul><ul><li>High sample variability </li></ul></ul><ul><ul><li>Large chemical diversity </li></ul></ul>
  16. 16. Target analysis Profiling Screening “ Accuracy Scope
  17. 17. <ul><ul><li>Scientifically most relevant </li></ul></ul><ul><ul><li>approach… </li></ul></ul>… true target analysis in combination with data from other ‘omics’ experiments.
  18. 18. Method requirements <ul><ul><li>Correct sample preparation </li></ul></ul><ul><ul><li>Accurate analysis </li></ul></ul><ul><ul><li>Fast data handling </li></ul></ul>
  19. 19. <ul><ul><li>Sample preparation </li></ul></ul>Too often the bottleneck…
  20. 20. Go for automated solutions that apply ‘ state-of-the-art’ sampler robotics.
  21. 21. <ul><ul><li>Target analysis </li></ul></ul>High throughput required…
  22. 22. Go for GC in combination with the selectivity of MS/MS in h-SRM mode.
  23. 23. <ul><ul><li>Data management </li></ul></ul>Go for automated data reporting solutions with direct LIMS interfacing.
  24. 24. Conclusion
  25. 25. Expert chromatographic knowledge is mandatory for you to decide which strategy to apply so you can be sure that the results you report are scientifically correct. Visit www.is-x.be for more details.

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