Lab assignment 1 revised 2


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Lab assignment 1 revised 2

  1. 1. Spring Semester 2011-12 University of Puerto Rico - Cayey RISE Program Instructions for Laboratory Writing Assignments1. For each assignment you should write a summaryparagraph of 8-10 sentences and a range from 160 -170 words.2. A template for each assignment is attached. Once you complete the template email it to Dr. Elena Gonzalez; she will grade it using the rubric below and return it to you. Within the next two days, you will email it to me with the corrections incorporated. In your summary include a reference to each one of the criteria in the rubric below.3. Email subject should include: Your register number; first name; Assignment # and hand-in date. Example: 1. Lysander Assignment 1 February 10 Rubric for Assessing Laboratory Summaries Name Grade Date Points Assignment Number 30 Points Total A. PURPOSE OF THE LAB 1 2 3 4 5 TECHNIQUE Refers to purpose and objectives of the lab techniques B. BIOLOGICAL COMPETENCE Demonstrates knowledge of laboratory procedures Reports findings adequately C. ENGLISH COMPETENCE Uses correct grammar, syntax, spelling, and punctuation Demonstrates clarity and coherence D. CRITICAL THINKING In concluding identifies applications and or implications of the study
  2. 2. University of Puerto Rico - Cayey RISE ProgramTemplate for Laboratory Summaries of Assignment 1Biol. 4997-BiomedicalTechniquesDue dateFebruary 17, 2012Reg.# 15 Name Juan Carlos Torres Sánchez Date: February 17, 2012Paragraph 1. Microscopy and PhotomicrographyThe creation of the microscope has improved our science knowledge and discoveries many scientist havemade using this instrument. Microscopy has improved during the years and now there are many techniquesone can apply by using a microscope. They have been used for the discovery of many things, includingbacteria and many other types of cells or microorganisms. This first workshop was about microscopytechniques and photomicrography. Some techniques that we learned were phase contrast, dark field,brightfield and perspective. The objective of the workshop was to learn different microscopy techniques and to dophotomicrography of a given assignment. My assignment was to take a photomicrography of my cheek cells,which are the epithelial cells in our mouth, through the phase contrast technique. The results showed asuccessful photo of my cheek cells that demonstrated the nucleus and other organisms inside those cells. Onecould see bacteria eating the cell because it was dying. We learned all of these techniques at the workshopand they are an important part of science because they have helped us discover bacteria and cells that maycause harm to humans. Seeing these microorganisms through the microscope can help scientists study them,and find ways to make them harmless.Paragraph 2. Measurements – Micropipettes& Aseptic techniquesPipettes are used to accurately measure and dispense small volumes of liquid. Micro Pipettes are one of themost important tools for various procedures in a laboratory. The accuracy and precision of pipette decidesthe quality of test results perform in a particular laboratory. Aseptic technique is a set of specific practicesand procedures performed under carefully controlled conditions with the goal of minimizing contaminationby pathogens. These workshops consisted in working with micropipettes, aseptic techniques and bacteria,and mixing different amounts of volumes. We performed a streak plate, which is spreading out a colony ofbacteria on an agar plate until a single bacteria is found. Also, we did a bacterial smear; first by staining thebacteria and then seeing them through the microscope. The results for the streak plate were looked at a weeklater and it had a successful spread of bacteria. The pipets and aseptic techniques have been used in manyinvestigations; also, bacteria identification has been really helpful in determining for example the bacteriathat causes throat infection. All of those techniques and instruments have improved the quality of theexperiments and being able to stain bacteria was also another great technique because scientists could seewhich type of bacteria it was and know if it was dangerous or not.Paragraph 3. Workshop UNC - From DNA to ProteinThis workshop consisted in a three-day event where the main objective was to learn about TranslationalMedicine and some techniques in DNA and protein separation and detection. On the first day, we learnedhow to isolate our own DNA. The second day, we worked with oncogene amplification, separation, anddetection. We did two experiments, the first one, a Polymerase Chain Reaction, copied the DNA multipletimes. Secondly, we did electrophoresis, which runs the DNA on an agarose gel. Depending on the lines thatappeared on that gel, the person could determine if the subject had the oncogen or not. The third day, weworked with oncoprotein separation and detection. First, we ran the proteins using the Western Blottechnique. Then, we detected what type of protein we had; if we had oncoprotein or not. The resultsindicated bands that my patients had both oncogene and oncoprotein. These techniques can be used as a
  3. 3. diagnostic for patients who may have cancer. Also, translational medicine is an area of research that focusesits efforts to carry scientific knowledge from the bench to the bedside. The determination of the oncoproteincan lead to future work in determining if the person has cancer or not. This workshop helped us realize thatscientists are needed to create new diagnostic procedures for the patient’s routine checkup that can be fastand easy in the diagnoses of disease.