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macromolecularnanomachines
IN ACTION



       José Ramón Macías
Hi, my name is:



José Ramón Macías
                  Bioinformatician
macromolecularnanomachines
IN ACTION
Sequence
   Structure
Sequence
   Structure
       Function
10 nm




100 nm




1 mm
X-Ray Diffraction
Crystallography
conformational changes
Simian Virus 40
Large T antigen
Simian virus 40
(SV40)
Large T antigen
High       Middle     Long &     Short &
curvature   curvature   straight   straight
High       Middle     Long &     Short &
curvature   curvature   straight   straight
Bacteriophage SPP1 replicative
         Helicase G40P
D. melanogaster
26S proteasome
26S proteasome
X-Ray               High       Requires
Crystallography      Resolution   Crystals   
X-Ray               High       Requires
Crystallography      Resolution   Crystals     

 3D Electron          Crystals ...
X-Ray               High       Requires
Crystallography      Resolution   Crystals     

 3D Electron          Crystals ...
X-Ray               High       Requires
Crystallography      Resolution   Crystals     

 3D Electron          Crystals ...
X-Ray               High       Requires
Crystallography      Resolution   Crystals     

 3D Electron          Crystals ...
X-Ray               High         Requires
Crystallography      Resolution     Crystals     

 3D Electron          Cryst...
3D-EM model   Atomic model
Hybrid model
GroEL chaperonin
Anti-tumoral
P53 transcription factor
Electron
 Microscopy
      Data
         Bank
363

“since June 2002, the number
 of structures has increased
 steadily.”                           266



              ...
Today there are
                                     629 entries




 Single
Particle   Icosahedral
                      ...
1 out of 3 structures
      are covered by more than
      one entry




369
DASx3DEM & PeppeR
http://biocomp.cnb.csic.es/das/PeppeR
Sequence
   Structure
adopting different conformations
                  is what leads to function
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
Macromolecular Nanomachines In Action
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Macromolecular Nanomachines In Action

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Published on

20091116-17
Curso de Genómica Aplicada a la Medicina Clínica (MADRID)
---
Las macromoléculas se comportan como diminutas máquinas para realizar sus funciones biológicas dentro de las células.
Si bien la estructura (en los diferentes niveles) viede dada por la secuencia de las proteínas, la función más que a la propia estructura la deben a las diferentes conformaciones que pueden adoptar.
Tanto la difracción de rayos X, como las técnicas de microscopía electrónica son capaces de resolver las estructuras 3D de un amplio rango de biomoleculas. Combinandolas obtenemos "modelos híbridos" que se benefician de las mejores caracteristicas de estas técnicas que se complementan.
PeppeR es una aplicación de integración de datos, que permite ver los modelos híbridos almacenados en EMDB, junto con anotaciones de otras muchas bases de datos de genomica, proteomica, etc.

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Transcript of "Macromolecular Nanomachines In Action"

  1. 1. macromolecularnanomachines IN ACTION José Ramón Macías
  2. 2. Hi, my name is: José Ramón Macías Bioinformatician
  3. 3. macromolecularnanomachines IN ACTION
  4. 4. Sequence Structure
  5. 5. Sequence Structure Function
  6. 6. 10 nm 100 nm 1 mm
  7. 7. X-Ray Diffraction Crystallography
  8. 8. conformational changes
  9. 9. Simian Virus 40 Large T antigen
  10. 10. Simian virus 40 (SV40)
  11. 11. Large T antigen
  12. 12. High Middle Long & Short & curvature curvature straight straight
  13. 13. High Middle Long & Short & curvature curvature straight straight
  14. 14. Bacteriophage SPP1 replicative Helicase G40P
  15. 15. D. melanogaster 26S proteasome
  16. 16. 26S proteasome
  17. 17. X-Ray High Requires Crystallography  Resolution Crystals 
  18. 18. X-Ray High Requires Crystallography  Resolution Crystals  3D Electron Crystals Medium Microscopy  Not required Resolution 
  19. 19. X-Ray High Requires Crystallography  Resolution Crystals  3D Electron Crystals Medium Microscopy  Not required Resolution 
  20. 20. X-Ray High Requires Crystallography  Resolution Crystals  3D Electron Crystals Medium Microscopy  Not required Resolution 
  21. 21. X-Ray High Requires Crystallography  Resolution Crystals  3D Electron Crystals Medium Microscopy  Not required Resolution  Hybrid Models
  22. 22. X-Ray High Requires Crystallography  Resolution Crystals  3D Electron Crystals Medium Microscopy  Not required Resolution  Fitting Hybrid Models
  23. 23. 3D-EM model Atomic model
  24. 24. Hybrid model
  25. 25. GroEL chaperonin
  26. 26. Anti-tumoral P53 transcription factor
  27. 27. Electron Microscopy Data Bank
  28. 28. 363 “since June 2002, the number of structures has increased steadily.” 266 193 117 67 40 10 2002 2003 2004 2005 2006 2007 2008
  29. 29. Today there are 629 entries Single Particle Icosahedral Helical Individual Structure 2D Crystal
  30. 30. 1 out of 3 structures are covered by more than one entry 369
  31. 31. DASx3DEM & PeppeR http://biocomp.cnb.csic.es/das/PeppeR
  32. 32. Sequence Structure
  33. 33. adopting different conformations is what leads to function
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