Your SlideShare is downloading. ×
Original Research Presentation
Upcoming SlideShare
Loading in...5

Thanks for flagging this SlideShare!

Oops! An error has occurred.

Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Original Research Presentation


Published on

Original research presented orally at the 2006 Southeastern Psychological Association Meeting

Original research presented orally at the 2006 Southeastern Psychological Association Meeting

  • Be the first to comment

  • Be the first to like this

No Downloads
Total Views
On Slideshare
From Embeds
Number of Embeds
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

No notes for slide


  • 1. Effect of a GABA Antagonist on Ethanol-Induced Taste Aversions Joseph Tkacz , M.S. Stephen H. Hobbs , Ph.D. Ralph L. Elkins , Ph.D.
  • 2. Conditioned Taste Aversions (CTA)
    • What is a taste aversion?
    • Lab Procedure
      • Pair flavored water (CS) with LiCl (UCS)
      • Then, assess acceptance/rejection of CS
    • Applications
      • Treatment for substance abuse/dependence
  • 3. Taste-Aversion Prone (TAP) and Taste-Aversion Resistant (TAR) Rats
    • “ Problem” of individual differences
      • Do these have a genetic basis?
    • Selective breeding program with rats
      • Saccharin (CS) + Cyclophosphamide (UCS)
      • Selectively breed those with stronger/weaker aversions
    • Result:
      • TAP animals -> Avoid saccharin
      • TAR animals -> Consume saccharin
  • 4.  
  • 5. TAP and TAR Lines Cont.
    • TAP and TAR rats do not differ on other types of learning
    • Line differences are maintained with other CSs
    • Line difference are maintained with other UCSs
      • Notably, even drugs of abuse: alcohol, cocaine, nicotine
      • Why?
  • 6. Research with Animal Lines
  • 7. Research with Animal Lines Cont.
  • 8. Our interest: What mechanisms are involved in the aversive response to ethanol by TAP animals?
  • 9. The GABA Receptor Complex
    • GABA is found in almost every region of brain
    • It is the main inhibitory neurotransmitter in the CNS
    • Benzodiazepines, barbiturates and alcohol, can bind to the GABA receptor complex
  • 10. Purpose and Hypothesis
    • Determine if the GABA system plays a role in the aversive response to alcohol
    • H 1 – Antagonizing the GABA receptor site will reduce the aversiveness of alcohol, as indicated by weakened rejection of saccharin in a CTA paradigm.
  • 11. Method- Subjects
    • 50 TAP Sprague-Dawley derived rats
    • Animals were allowed to eat ad libitum throughout the course of the experiment
    • 12:12 light-dark schedule with lights on at 0600 EST; temperature of 23ºC +/- 2º.
  • 12. Method- Materials
    • Picrotoxin
      • a white, crystalline substance that has been shown to effectively antagonize the GABA A receptor site
  • 13. Pre Post Consummatory Forced Consummatory Dependent Group Injection Drink Injection Variable Hypothesis (CS) ( UCS) _______________________________________________________________________________ A Strong Conditioning Saline -> Saccharin -> Ethanol -> Two Bottle Aversion Test B Weaker Low Drug Picrotoxin -> Saccharin -> Ethanol -> Two Bottle Aversion Experimental .5 mg/kg Test C Weakest High Drug Picrotoxin -> Saccharin -> Ethanol -> Two Bottle Aversion Experimental 1 mg/kg Test D No Pseudo- Picrotoxin -> H20 -> Ethanol -> Two Bottle Aversion Conditioning 1 mg/kg Test Control E No Injection Picrotoxin -> Saccharin -> Saline -> Two Bottle Aversion Control 1 mg/kg Test
  • 14.  
  • 15. Discussion
    • The primary hypothesis was supported:
      • Picrotoxin CTA Groups > Saline CTA Group
      • Picrotoxin CTA Groups = Control Groups
  • 16. Conclusion
    • Aversive component of alcohol and GABA
    • The taste aversion paradigm
    • Picrotoxin dosage
  • 17. Future Research
    • Taste aversions to other UCSs, especially substances of abuse
    • Examining the TAR rats