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Synthesis of electrospun cellulose fibers corrected
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  • 1. Synthesis of electrospun cellulose acetate fiber membranes for successful protein separation José Cruz-Arzon1, Delaine Zayas-Bazan1, Nelson Granda-Paz2, and Wilfredo Otaño-Rivera3,4 1-Department of Biology, University of Puerto Rico at Cayey, Puerto Rico 2-Department of Chemistry, University of Puerto Rico at Mayagüez, Puerto Rico 3- Department of Physics-Mathematics, University of Puerto Rico at Cayey, Puerto Rico 4-Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico
  • 2. Background • Nanotechnology- Nanofibers • Electrospinning • SEM, FTIR • Deacetylation
  • 3. Background • Cellulose • Cellulose Acetate for protein separation
  • 4. Problem • Is it possible to prepare electrospun cellulose fibers that could be used for separation of proteins producing high flow and mass transfer?
  • 5. Hypothesis • Control of chemical and physical parameters in cellulose electrospinning synthesis will allow to vary pore size distribution of electrospun membranes. • Pore size distribution is a factor that can be used to produce a high mass transfer, high flow and specificity in the separation of proteins.
  • 6. Objectives • Synthesize cellulose acetate fibers by electrospinning. • Analyze fiber properties by SEM & FTIR. • Remove acetyl groups • Control chemical and physical electrospinning process parameters to vary pore size distribution of membranes.
  • 7. Methodology Solution Preparation Electrospinning SEM Analysis FTIR, EDS analysis Variations in Parameters SEM analysis
  • 8. Methodology • Solution preparation ▫ Solvent: Acetone/ Dimethylformamide/ Ethanol (2:2:1) ▫ 15%, 17%, 20%, 21% Cellulose Acetate ▫ 24 hrs ▫ Bain Marie (50ºC) • Viscosimeter
  • 9. Methodology • Electrospinning ▫ ▫ ▫ ▫ 18 cm 20.0 kV 0.5 mL/ hr 1 mL
  • 10. Methodology • Analysis ▫ Scanning Electron Microscope (SEM) ▫ Infrared Spectroscopy (IR) ▫ Electron Dispersive Spectroscopy (EDS)
  • 11. Methodology • Variations in Process Parameters ▫ Amount of acetyl cellulose and amount of solvent ▫ Speed of rotor collector ▫ Humidity control ▫ Voltage and needle to collector distance
  • 12. 15% CA fibers
  • 13. 17% CA fibers
  • 14. 20% CA fibers
  • 15. 21% CA fibers
  • 16. Future Works • Measure fiber diameter and pore size distribution • Deacetylation • Protein separation
  • 17. Acknowledgements • UPR at Cayey • RISE Program • NAMAS Lab • Mr. Carlos Pérez
  • 18. Thank You Questions?