Synthesis of electrospun cellulose acetate
fiber membranes for successful protein
separation
José Cruz-Arzon1, Delaine Zay...
Background
• Nanotechnology- Nanofibers
• Electrospinning

• SEM, FTIR
• Deacetylation
Background
• Cellulose

• Cellulose Acetate for protein separation
Problem
• Is it possible to prepare electrospun cellulose
fibers that could be used for separation of
proteins producing h...
Hypothesis
• Control of chemical and physical parameters in
cellulose electrospinning synthesis will allow to
vary pore si...
Objectives
• Synthesize cellulose acetate fibers by electrospinning.
• Analyze fiber properties by SEM & FTIR.

• Remove a...
Methodology

Solution
Preparation

Electrospinning

SEM Analysis

FTIR, EDS
analysis

Variations in
Parameters

SEM analys...
Methodology
• Solution preparation
▫ Solvent: Acetone/ Dimethylformamide/ Ethanol
(2:2:1)
▫ 15%, 17%, 20%, 21% Cellulose A...
Methodology
• Electrospinning
▫
▫
▫
▫

18 cm
20.0 kV
0.5 mL/ hr
1 mL
Methodology
• Analysis
▫ Scanning Electron Microscope (SEM)
▫ Infrared Spectroscopy (IR)
▫ Electron Dispersive Spectroscop...
Methodology
• Variations in Process Parameters
▫ Amount of acetyl cellulose and amount of solvent
▫ Speed of rotor collect...
15% CA fibers
17% CA fibers
20% CA fibers
21% CA fibers
Future Works
• Measure fiber diameter and pore size distribution
• Deacetylation

• Protein separation
Acknowledgements
• UPR at Cayey
• RISE Program
• NAMAS Lab
• Mr. Carlos Pérez
Thank You
Questions?
Synthesis of electrospun cellulose fibers corrected
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Synthesis of electrospun cellulose fibers corrected

  1. 1. Synthesis of electrospun cellulose acetate fiber membranes for successful protein separation José Cruz-Arzon1, Delaine Zayas-Bazan1, Nelson Granda-Paz2, and Wilfredo Otaño-Rivera3,4 1-Department of Biology, University of Puerto Rico at Cayey, Puerto Rico 2-Department of Chemistry, University of Puerto Rico at Mayagüez, Puerto Rico 3- Department of Physics-Mathematics, University of Puerto Rico at Cayey, Puerto Rico 4-Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico
  2. 2. Background • Nanotechnology- Nanofibers • Electrospinning • SEM, FTIR • Deacetylation
  3. 3. Background • Cellulose • Cellulose Acetate for protein separation
  4. 4. Problem • Is it possible to prepare electrospun cellulose fibers that could be used for separation of proteins producing high flow and mass transfer?
  5. 5. Hypothesis • Control of chemical and physical parameters in cellulose electrospinning synthesis will allow to vary pore size distribution of electrospun membranes. • Pore size distribution is a factor that can be used to produce a high mass transfer, high flow and specificity in the separation of proteins.
  6. 6. Objectives • Synthesize cellulose acetate fibers by electrospinning. • Analyze fiber properties by SEM & FTIR. • Remove acetyl groups • Control chemical and physical electrospinning process parameters to vary pore size distribution of membranes.
  7. 7. Methodology Solution Preparation Electrospinning SEM Analysis FTIR, EDS analysis Variations in Parameters SEM analysis
  8. 8. Methodology • Solution preparation ▫ Solvent: Acetone/ Dimethylformamide/ Ethanol (2:2:1) ▫ 15%, 17%, 20%, 21% Cellulose Acetate ▫ 24 hrs ▫ Bain Marie (50ºC) • Viscosimeter
  9. 9. Methodology • Electrospinning ▫ ▫ ▫ ▫ 18 cm 20.0 kV 0.5 mL/ hr 1 mL
  10. 10. Methodology • Analysis ▫ Scanning Electron Microscope (SEM) ▫ Infrared Spectroscopy (IR) ▫ Electron Dispersive Spectroscopy (EDS)
  11. 11. Methodology • Variations in Process Parameters ▫ Amount of acetyl cellulose and amount of solvent ▫ Speed of rotor collector ▫ Humidity control ▫ Voltage and needle to collector distance
  12. 12. 15% CA fibers
  13. 13. 17% CA fibers
  14. 14. 20% CA fibers
  15. 15. 21% CA fibers
  16. 16. Future Works • Measure fiber diameter and pore size distribution • Deacetylation • Protein separation
  17. 17. Acknowledgements • UPR at Cayey • RISE Program • NAMAS Lab • Mr. Carlos Pérez
  18. 18. Thank You Questions?

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