Embryo Transfer in Alpacas

Basic Concepts About ET in Alpacas Jorge Reyna BSc (Hons), MScVetSc (Sydney Univ.) Alpaca Breeding Technologies

What is embryo transfer? Donor Recipients

ET steps
Herd evaluation (donors and recipients)
Superovulation and mating of donors
Flushing
Isolation and evaluation of embryos
Recipient synchronisation
Embryo transfer

Herd evaluation
Four essential criteria:
1. Rectal accessibility
2. Reproductive tract
3. Ovarian examinations
4. Cervical accessibility
HE is essential due to:
Investment in hormones (2) Investment in laboratory materials
(3) Time-consuming nature of ET (4) Service fees

Superovulation and Mating of Donors
Growth stimulation of several follicles at a time
Ovulation induction of stimulated follicles
Mating in order to fertilise the oocytes

How to superovulate?
To induce follicular growth multiple doses of FSH are required, 3-5 days 12 hr interval.
To induce ovulation of these follicles a LH injection is required (GnRH).
After treatment a luteolytic dose of PGF2 α is required.

Recipient synchronisation
Reproductive status must be synchronised between donors and recipients. For that, a single injection of GnRH is used

Flushing of embryos

Embryo assessment

Embryo transfer

Recommendations
Minimise stress in the herd before, during and after ET.
Make sure animals are in a good body condition and healthy.
Donors and recipients must be animals of proven fertility.
Two to four weeks before starting a superovulatory treatment is important to supplement animals with lupin seeds.

Contact us
Jorge Reyna
BSc (Hons), MScVetSc (Sydney Univ.)
2/122 Newington Rd, Petersham, NSW 2049 Australia
Phone: +61 2 9568 1370,
Mobile: +61 428 ALPACA
E-mail: [email_address]

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Embryo Transfer in Alpacas

by jorge_llama_guy on Mar 22, 2007

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