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Embryo Transfer in Alpacas
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Transcript

  • 1. Basic Concepts About ET in Alpacas Jorge Reyna BSc (Hons), MScVetSc (Sydney Univ.) Alpaca Breeding Technologies
  • 2. What is embryo transfer? Donor Recipients
  • 3. ET steps
    • Herd evaluation (donors and recipients)
    • Superovulation and mating of donors
    • Flushing
    • Isolation and evaluation of embryos
    • Recipient synchronisation
    • Embryo transfer
  • 4. Herd evaluation
    • Four essential criteria:
    • 1. Rectal accessibility
    • 2. Reproductive tract
    • 3. Ovarian examinations
    • 4. Cervical accessibility
    • HE is essential due to:
    • Investment in hormones (2) Investment in laboratory materials
    • (3) Time-consuming nature of ET (4) Service fees
  • 5. Superovulation and Mating of Donors
    • Growth stimulation of several follicles at a time
    • Ovulation induction of stimulated follicles
    • Mating in order to fertilise the oocytes
  • 6. How to superovulate?
    • To induce follicular growth multiple doses of FSH are required, 3-5 days 12 hr interval.
    • To induce ovulation of these follicles a LH injection is required (GnRH).
    • After treatment a luteolytic dose of PGF2 α is required.
  • 7. Recipient synchronisation
    • Reproductive status must be synchronised between donors and recipients. For that, a single injection of GnRH is used
  • 8. Flushing of embryos
  • 9. Embryo assessment
  • 10. Embryo transfer
  • 11. Recommendations
    • Minimise stress in the herd before, during and after ET.
    • Make sure animals are in a good body condition and healthy.
    • Donors and recipients must be animals of proven fertility.
    • Two to four weeks before starting a superovulatory treatment is important to supplement animals with lupin seeds.
  • 12. Contact us
    • Jorge Reyna
    • BSc (Hons), MScVetSc (Sydney Univ.)
    • 2/122 Newington Rd, Petersham, NSW 2049 Australia
    • Phone: +61 2 9568 1370,
    • Mobile: +61 428 ALPACA
    • E-mail: [email_address]
  • 13.
    • Copyrights ®ArtMinds Digital Media Solutions