Sample illumination is via transmitted white light . Simplicity of setup with only basic equipment required. No sample preparation required, allowing viewing of live cells Bright Field Microscope Specific Use Advantage Types of Light Microscope
Transparent specimens Cell parts are transparent Does not require staining living cells can be examined in their natural state Phase Contrast Microscope Live and biological samples Study of crystals and crystal defects Quality of image is impressive Raised features Dark field Microscope
Living cells and internal components are contrasted against the background giving greater definition and detail of cell structure Detect structures and molecules within the cell Fluorescent Microscope
Used for dissection to get a better look at the larger specimen. Gives true color with 20x and 40x power using 10x eyepieces and rotating turret of 2x and 4x objectives Dissecting Microscope Allow the specimen to remain active for long periods. Allow to observe a living cells or organisms at the bottom of a large container Inverted Microscope Allows the specimen to be seen more clearly Do not mask or otherwise obstruct the objective and condenser apertures Differential Interference Contrast Microscope
designed long working distance objectives & wide field eyepieces produce an extremely large, brilliant & bright images. Light in weight, fixed achromatic objectives 2x or 3x Stereo Microscope
When using compound microscope and you come with very high magnifications with transmitted light, point objects are seen as fuzzy discs surrounded by diffraction rings or the so called airy-disc.
Limitations of lens design which can result in increased magnification without increased resolution resulting to image that is larger but not clearer and could not present more detailed information.
Two objects must be 0.1 mm apart so that they will be perceived as two, there are lens deign that even though objects appears 0.1 mm apart, the edges becomes blurry that we detect two objects as single.