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Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).

Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).

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    Talk3 - Zebrafish Talk3 - Zebrafish Presentation Transcript

    • The Effects of fipronil on notochord development in Zebrafish Joshua Mendoza-Elias Biology 205L Experiments in Developmental Biology Fall 2006
    • Backgr ound on Fipronil
      • Pesticide
      • Blocks GABA receptors
      • Teratogen
      • Found to cause notochord damage in zebrafish --> Chordates
      • GABA R active at 30 hours AEL
      Stehr et al. 2006
    • Background- Accordian Phenotype
    • Question :
      • (i.) Early on, will exposure to Fipronil cause neurodegeneration in notochord?
      • (ii.) Will the damage be necrotic tissue?
      • (iii.) Will their be damage elsewhere?
      (i.) Fipronil damages notochord (ii.) Damage will result in necrotic tissue Hypothesis:
    • Materials & Methods Control (0.1% acetone) 0.7  M Fipronil (0.1% acetone) 1.1  M Fipronil (0.1% acetone) 2.3  M Fipronil (0.1% acetone) Notochord Morphology Acridine Orange Staining Behavioral Response
    • Results-Behavioral Response
      • 36 hrs - Control
    • 36 hrs - 0.7  M
    • 36 hrs - 1.1  M
    • 36 hrs - 2.3  M
    • Results-Behavioral Response
      • 48 hrs - Control
    • 48 hrs - 0.7  M
    • 48 hrs - 1.1  M
    • 48 hrs - 2.3  M
    • Results-Notochord Morphology
      • Control: 36 hrs, 48 hrs
    • 36 hrs, 48 hrs - 0.7  M
    • 36 hrs, 48 hrs - 1.1  M
    • 36 hrs, 48 hrs – 2.3  M
    • Results 39 Total moderate: Total severe: 15 Total WT: 7 Total moderate: Total severe: Total WT: 36 hrs 48 hrs 23 31 10 24.59% 69.93% 11.47% 35.93% 48.43% 15.62%
    • Results-Acridine Orange Staining
    • Discussion
      • Notochord damage first occurs anteriorly
      • Higher concentration = more damage
      • Higher concentration = damage sooner
      •  Exposure time = more damage
    • Future Directions
      • Anti-body staining
      • Examine upper notochord
      • Circulation
    • References: [1] Stehr. C., Linbo, L.L., Incardona, J. P, Scholz, N. L. 2006. The developmental neurotoxicity of fipronil: notochord degeneration and locomotor defects in zebrafish embryos and larvae. Toxicological Sciences . 2006 92 ( 1): 270 -278. [2] Incardona, J., Stehr, C., Linbo, T., Scholz, N. Fipronil causes locomotor defects in developing zebrafish. 2006. Marine Environmental Research. 2006. 62 : S74 -S75. [3] Cole, Loretta M., Russel A. Nicholson, and John E. Casida. 1993. Action of phenylpyrazole insecticides at the GABA-gated chloride channel. Pest. Biochemical Physiology . 46 : 47-54. [4] Hamon, Nicholas, Richard Shaw, and Henry Yang. 1996. Worldwide development of fipronil insecticide. Proc.-Beltwide Cotton Conference. 2 : 759-765. [5] Colliot, F., K.A. Kukowskim P.W. Hawkins, and D.A. Robers. 1992. Fipronil: a new soil and foliar broad spectrum insecticide. Brighton Crop Proteonomics. Conf.-Pests Dis. 1 :29-34.
    • Acknowledgements
      • Dr. B
      • Dr. P
      • Nicole Roy
      • Wendy Beane
      • Monica Zhang