Talk3 - Zebrafish

1,036 views

Published on

Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
1,036
On SlideShare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
17
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Talk3 - Zebrafish

  1. 1. The Effects of fipronil on notochord development in Zebrafish Joshua Mendoza-Elias Biology 205L Experiments in Developmental Biology Fall 2006
  2. 2. Backgr ound on Fipronil <ul><li>Pesticide </li></ul><ul><li>Blocks GABA receptors </li></ul><ul><li>Teratogen </li></ul><ul><li>Found to cause notochord damage in zebrafish --> Chordates </li></ul><ul><li>GABA R active at 30 hours AEL </li></ul>Stehr et al. 2006
  3. 3. Background- Accordian Phenotype
  4. 4. Question : <ul><li>(i.) Early on, will exposure to Fipronil cause neurodegeneration in notochord? </li></ul><ul><li>(ii.) Will the damage be necrotic tissue? </li></ul><ul><li>(iii.) Will their be damage elsewhere? </li></ul>(i.) Fipronil damages notochord (ii.) Damage will result in necrotic tissue Hypothesis:
  5. 5. Materials & Methods Control (0.1% acetone) 0.7  M Fipronil (0.1% acetone) 1.1  M Fipronil (0.1% acetone) 2.3  M Fipronil (0.1% acetone) Notochord Morphology Acridine Orange Staining Behavioral Response
  6. 6. Results-Behavioral Response <ul><li>36 hrs - Control </li></ul>
  7. 7. 36 hrs - 0.7  M
  8. 8. 36 hrs - 1.1  M
  9. 9. 36 hrs - 2.3  M
  10. 10. Results-Behavioral Response <ul><li>48 hrs - Control </li></ul>
  11. 11. 48 hrs - 0.7  M
  12. 12. 48 hrs - 1.1  M
  13. 13. 48 hrs - 2.3  M
  14. 14. Results-Notochord Morphology <ul><li>Control: 36 hrs, 48 hrs </li></ul>
  15. 15. 36 hrs, 48 hrs - 0.7  M
  16. 16. 36 hrs, 48 hrs - 1.1  M
  17. 17. 36 hrs, 48 hrs – 2.3  M
  18. 18. Results 39 Total moderate: Total severe: 15 Total WT: 7 Total moderate: Total severe: Total WT: 36 hrs 48 hrs 23 31 10 24.59% 69.93% 11.47% 35.93% 48.43% 15.62%
  19. 19. Results-Acridine Orange Staining
  20. 20. Discussion <ul><li>Notochord damage first occurs anteriorly </li></ul><ul><li>Higher concentration = more damage </li></ul><ul><li>Higher concentration = damage sooner </li></ul><ul><li>  Exposure time = more damage </li></ul>
  21. 21. Future Directions <ul><li>Anti-body staining </li></ul><ul><li>Examine upper notochord </li></ul><ul><li>Circulation </li></ul>
  22. 22. References: [1] Stehr. C., Linbo, L.L., Incardona, J. P, Scholz, N. L. 2006. The developmental neurotoxicity of fipronil: notochord degeneration and locomotor defects in zebrafish embryos and larvae. Toxicological Sciences . 2006 92 ( 1): 270 -278. [2] Incardona, J., Stehr, C., Linbo, T., Scholz, N. Fipronil causes locomotor defects in developing zebrafish. 2006. Marine Environmental Research. 2006. 62 : S74 -S75. [3] Cole, Loretta M., Russel A. Nicholson, and John E. Casida. 1993. Action of phenylpyrazole insecticides at the GABA-gated chloride channel. Pest. Biochemical Physiology . 46 : 47-54. [4] Hamon, Nicholas, Richard Shaw, and Henry Yang. 1996. Worldwide development of fipronil insecticide. Proc.-Beltwide Cotton Conference. 2 : 759-765. [5] Colliot, F., K.A. Kukowskim P.W. Hawkins, and D.A. Robers. 1992. Fipronil: a new soil and foliar broad spectrum insecticide. Brighton Crop Proteonomics. Conf.-Pests Dis. 1 :29-34.
  23. 23. Acknowledgements <ul><li>Dr. B </li></ul><ul><li>Dr. P </li></ul><ul><li>Nicole Roy </li></ul><ul><li>Wendy Beane </li></ul><ul><li>Monica Zhang </li></ul>

×