David Wadsworth Vice President Global Product Strategy

A Passion for Noni Research

7 bottles/day/7 years 2155 days 17,885 bottles 4,471 cases 7 Liters of TNJ/Day

$300,000. 2 Years.

Obsessed with Noni Research

Brunswick Labs Press Release “ There is a lot of ‘chatter’ in the marketplace concerning who/what has the highest ORAC value. It is very important to understand the difficulty in making direct comparisons... On behalf of Brunswick Laboratories, I apologize for the confusion... ” Jim Nichols, President

“ There is a lot of ‘chatter’ in the marketplace concerning who/what has the highest ORAC value. It is very important to understand the difficulty in making direct comparisons...

On behalf of Brunswick Laboratories, I apologize for the confusion... ”

Jim Nichols, President

Placebo is TNJ without Noni

TNJ is a Superior Antioxidant

TNJ Aids in Quality of Life

TNJ Increases Energy & Physical Performance

Human Clinical Studies – to Date Keeping score: TAHITIAN NONI Juice Competition (all) 14 and counting 0 (44 for topicals) = 58 © 2007 TAHITIAN NONI INTERNATIONAL

Keeping score:

TAHITIAN NONI Juice Competition (all)

14 and counting 0

(44 for topicals)

= 58

The effect of TAHITIAN NONI® Juice on antioxidant and immune function Method 12 healthy volunteers 330 ml TNJ for 8 weeks Pre and post blood tests for malondialdehyde (MDA), IL-2, natural killer (NK) cell activity Results No adverse effects Significant reduction in MDA—demonstrating an antioxidant effect Significant increase in IL-2 and NK cell activity—demonstrating an improvement in immune function.

Method

12 healthy volunteers

330 ml TNJ for 8 weeks

Pre and post blood tests for malondialdehyde (MDA), IL-2, natural killer (NK) cell activity

Results

No adverse effects

Significant reduction in MDA—demonstrating an antioxidant effect

Significant increase in IL-2 and NK cell activity—demonstrating an improvement in immune function.

Testing of antioxidant activity of TNJ on blood plasma and erythrocytes Method Thirty healthy volunteers drank either 200 mL of TNJ, 200 mL orange juice, or 500 mL water330 ml TNJ for 8 weeks Pre and post (1 hr) blood tests (potentiometric) for antioxidant activity (AOA) Results Plasma AOA two times greater with TNJ than orange juice Erythrocyte AOA five times greater with TNJ than orange juice.

Method

Thirty healthy volunteers drank either 200 mL of TNJ, 200 mL orange juice, or 500 mL water330 ml TNJ for 8 weeks

Pre and post (1 hr) blood tests (potentiometric) for antioxidant activity (AOA)

Results

Plasma AOA two times greater with TNJ than orange juice

Erythrocyte AOA five times greater with TNJ than orange juice.

2001-2006 Journal Publications

2007 Journal Publications

2008 Journal Publications—so far

TNI and Its Research on Noni Johannes Westendorf University of Hamburg Germany

Soon after its foundation, Morinda Inc. began with systematic research on noni. They built up their own Department of Research and Development (R&D) and a Health and Technical Advisory Board (HTAB) , later replaced by the Global Advisory Board (GAB).

Foundation of the company Morinda Inc. Ralph Heinicke publication on noni Furusawa and Hirazumi Anti-cancer effects of noni

Topic of research No. of publications % of total ______________________________________________________ Ethnopharmacology 84 19.5 Chemistry 74 17.2 Toxicology 42 9.76 Cancer&Immunsystem 37 8.60 Pharmacology 33 7.67 Inflammation 25 5.81 Antimicrobial 24 5.58 Antioxidant 24 5.58 Review articles 24 5.58 Botany of Noni 18 4.18 Other topics 17 3.95 Noni Plant cell culture 16 3.72 Hormonal action of Noni 7 1.62 Product quality 5 1.16 Total 430 100

Ethno-Pharmacology

The old Polynesians took noni juice to increase their endurance at long fishing trips. Do we have evidence that noni juice increases the endurance?

Experiment on athletes (N=20)

Cortisol plasma level Day time Cortisol blood level (ng/ml) Day light Thalamus Hypothalamus Pituitary Adrenals Cortisol ACTH CRF Start End

200 ml TNJ

Jonas Student Susanne Student Johannes Supervisor

Simla Postdoc

Cortisol blood level (mean of N=4) Values are Mean of 4

The endurance is very much dependent on the body's load with antioxidants. Endogenous antioxidants Katalase Superoxid dismutase Glutathion NADH/NADPH Exogenous antioxidants Vitamin C Vitamin E Flavonoids Catechines Selenium et c.

Exogenous antioxidants have to be absorbed and distributed to the site, where the radicals are, in order to be effective Endogenous antioxidants are always present in our body. Their concentration can be influenced by exogenous compounds (food ingredients)

Role of Antioxidants O 2 H 2 O + CO 2 SAR Hydroxyl- radicals Tissue damage Antioxidants destroy Glucose Inflammation Ageing Cancer

Depletion of antioxidants causes exhaustion (fatigue)

Measurement of Antioxidant capacity The measurement of the antioxidant capacity (AOA) should be performed inside of our body in order to be predictive

Antioxidative potency of blood, one hour after intake of 200ml of test compound 20 volunteers per group Serum Red blood cells O.118 0.055 0.6 2.7

Research on the safety of TNJ Acute toxicity in mice and rats highest dose in rats was equivalent to 7 l of TNJ in humans Subchronic toxicity in rats (90 days) Human trial with 96 volunteers Double blind placebo controlled study Group 1: 750 ml of 11% blending juices per day Group 2: 750 ml of 11% blending juices per day + 30ml TNJ Group 3: 750 ml of 11% blending juices per day +300ml TNJ Group 4: 750 ml TNJ per day The treatment period was over a full month None of these studies yielded any sign of toxic reactions

Acute toxicity in mice and rats highest dose in rats was equivalent to 7 l of TNJ in humans

Subchronic toxicity in rats (90 days)

Human trial with 96 volunteers Double blind placebo controlled study Group 1: 750 ml of 11% blending juices per day Group 2: 750 ml of 11% blending juices per day + 30ml TNJ Group 3: 750 ml of 11% blending juices per day +300ml TNJ Group 4: 750 ml TNJ per day The treatment period was over a full month

None of these studies yielded any sign of toxic reactions

Genotoxicity studies performed with TNJ In vitro short term assays Bacterial mutagenicity (Ames Test) Mutagenicity in mammalian cells (V79-HPRT test) Formation of DNA adducts after treatment of hepatocytes In vivo short term assays Measurement of DNA-damage (UDS and DNA- strand breaks) Micronuclei in mice Formation of DNA adducts after tretament of rats

In vitro short term assays Bacterial mutagenicity (Ames Test) Mutagenicity in mammalian cells (V79-HPRT test) Formation of DNA adducts after treatment of hepatocytes

In vivo short term assays Measurement of DNA-damage (UDS and DNA- strand breaks) Micronuclei in mice Formation of DNA adducts after tretament of rats

Animals on the Marquesa islands eat noni leaf and fruit. They are healthy, and especially their liver is very clean.

As a result of TNI’s intense studies on the safety of TNJ, the EU commission stated: “ Noni juice is safe for human consumption”

This statement was confirmed by the EU after some publications stated that noni juice could damage the liver.

Research on the quality of TNJ TNI invested enormous money and effort in the definition and maintenance of the quality of their noni products. The company controls and defines every step from the harvest until distribution of the products. Other companies often buy noni concentrates prepared at tropical islands far from their control, dilute it and add preservatives and artificial flavoring compounds.

CPX = (2 E ,4 Z ,7 Z )-Decatrienoic acid) Other polyunsaturated fatty acids (PUFA) Quercetin Scopoletin HPLC-chromatogram of a TNJ ethylacetat extract with marker compounds indicated

HPLC-Analysis of a competitor noni (black) and TNJ (red) Collection of peak and further analysis bc GC/MS

GC/MS analysis of peak isolated from HPLC run of the competitor noni

Summary Systematic research on noni began with TNI. This research is of top scientific standard. The results are published in peer-reviewed journals. No other juice company worldwide invested so much money and effort on research on their products.

Systematic research on noni began with TNI.

This research is of top scientific standard.

The results are published in peer-reviewed journals.

No other juice company worldwide invested so much money and effort on research on their products.

TNI’s research guarantees a constant quality of their noni products from the beginning to present. TNI’s research on the safety of noni, accepted by official governmental institutions worldwide, is the only one available. It guarantees that everybody can drink TAHITIAN NONI® Juice without fear, regardless of his or her physical or mental constitution.

TNI’s research guarantees a constant quality of their noni products from the beginning to present.

TNI’s research on the safety of noni, accepted by official governmental institutions worldwide, is the only one available.

It guarantees that everybody can drink TAHITIAN NONI® Juice without fear, regardless of his or her physical or mental constitution.

TNI’s research has confirmed the benefits of noni reported by the ancient Polynesians with modern scientific methods ( chemistry, preclinical laboratory experiments, clinical trials ). This research supports the IPCs with product knowledge, based on high quality science. It gives them the expertise to understand and explain the beneficial effects of noni.

TNI’s research has confirmed the benefits of noni reported by the ancient Polynesians with modern scientific methods ( chemistry, preclinical laboratory experiments, clinical trials ).

This research supports the IPCs with product knowledge, based on high quality science. It gives them the expertise to understand and explain the beneficial effects of noni.

Two Additional Novel Food Applications

Extension of Use of Fruit

Noni Leaf as Food

Benefits of Noni Leaf

Green tea infusion Noni leaf infusion

World Class Research