TNI and Its Research on Noni Johannes Westendorf University of Hamburg Germany
Soon after its foundation, Morinda Inc. began with systematic research on noni. They built up their own Department of Research and Development (R&D) and a Health and Technical Advisory Board (HTAB) , later replaced by the Global Advisory Board (GAB).
Foundation of the company Morinda Inc. Ralph Heinicke publication on noni Furusawa and Hirazumi Anti-cancer effects of noni
Topic of research No. of publications % of total ______________________________________________________ Ethnopharmacology 84 19.5 Chemistry 74 17.2 Toxicology 42 9.76 Cancer&Immunsystem 37 8.60 Pharmacology 33 7.67 Inflammation 25 5.81 Antimicrobial 24 5.58 Antioxidant 24 5.58 Review articles 24 5.58 Botany of Noni 18 4.18 Other topics 17 3.95 Noni Plant cell culture 16 3.72 Hormonal action of Noni 7 1.62 Product quality 5 1.16 Total 430 100
Cortisol blood level (mean of N=4) Values are Mean of 4
The endurance is very much dependent on the body's load with antioxidants. Endogenous antioxidants Katalase Superoxid dismutase Glutathion NADH/NADPH Exogenous antioxidants Vitamin C Vitamin E Flavonoids Catechines Selenium et c.
Exogenous antioxidants have to be absorbed and distributed to the site, where the radicals are, in order to be effective Endogenous antioxidants are always present in our body. Their concentration can be influenced by exogenous compounds (food ingredients)
Role of Antioxidants O 2 H 2 O + CO 2 SAR Hydroxyl- radicals Tissue damage Antioxidants destroy Glucose Inflammation Ageing Cancer
Depletion of antioxidants causes exhaustion (fatigue)
Measurement of Antioxidant capacity The measurement of the antioxidant capacity (AOA) should be performed inside of our body in order to be predictive
Antioxidative potency of blood, one hour after intake of 200ml of test compound 20 volunteers per group Serum Red blood cells O.118 0.055 0.6 2.7
Acute toxicity in mice and rats highest dose in rats was equivalent to 7 l of TNJ in humans
Subchronic toxicity in rats (90 days)
Human trial with 96 volunteers Double blind placebo controlled study Group 1: 750 ml of 11% blending juices per day Group 2: 750 ml of 11% blending juices per day + 30ml TNJ Group 3: 750 ml of 11% blending juices per day +300ml TNJ Group 4: 750 ml TNJ per day The treatment period was over a full month
None of these studies yielded any sign of toxic reactions
In vitro short term assays Bacterial mutagenicity (Ames Test) Mutagenicity in mammalian cells (V79-HPRT test) Formation of DNA adducts after treatment of hepatocytes
In vivo short term assays Measurement of DNA-damage (UDS and DNA- strand breaks) Micronuclei in mice Formation of DNA adducts after tretament of rats
Animals on the Marquesa islands eat noni leaf and fruit. They are healthy, and especially their liver is very clean.
As a result of TNI’s intense studies on the safety of TNJ, the EU commission stated: “ Noni juice is safe for human consumption”
This statement was confirmed by the EU after some publications stated that noni juice could damage the liver.
Research on the quality of TNJ TNI invested enormous money and effort in the definition and maintenance of the quality of their noni products. The company controls and defines every step from the harvest until distribution of the products. Other companies often buy noni concentrates prepared at tropical islands far from their control, dilute it and add preservatives and artificial flavoring compounds.
CPX = (2 E ,4 Z ,7 Z )-Decatrienoic acid) Other polyunsaturated fatty acids (PUFA) Quercetin Scopoletin HPLC-chromatogram of a TNJ ethylacetat extract with marker compounds indicated
HPLC-Analysis of a competitor noni (black) and TNJ (red) Collection of peak and further analysis bc GC/MS
GC/MS analysis of peak isolated from HPLC run of the competitor noni