Objectives
The objective of this lab is to understand how genes are expressed.
Materials
• 20 DNA Template Cards: these wi...
2. TAC,TTT,TTG,TTA,ACG,TGC,ATC
3. TAC,ATT,TGG,TGA,TGT,ATC
4. TAC,TCT,TCA,TCC,TCT,TCG,TAT,TAG,ATC
AUG AGA AGU AGG AGA AGC A...
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Protein synthesis lab

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Protein synthesis lab

  1. 1. Objectives The objective of this lab is to understand how genes are expressed. Materials • 20 DNA Template Cards: these will be kept on the supply table at all times. After all, DNA cannot leave the nucleus. 20 DNA Template Cards: these will be kept on the supply table at all times. After all, DNA cannot leave the nucleus. • Codon chart for sentences Codon chart for sentences Procedure 1. Your group will be assigned a number 2. Go to the table, with the DNA template cards 3. Find the card with your number 4. While staying in the nucleus (the table with the cards), transcribe the mRNA, from your DNA card, onto your lab report. 5. Return to your table. 6. Using the codon chart, translate your protein from the mRNA 7. Get another number from your teacher to make another protein. While staying in the nucleus (the table with the cards), transcribe the mRNA, from your DNA card, onto your lab report. 5. Return to your table. 6. Using the codon chart, translate your protein from the mRNA 7. Get another number from your teacher to make another protein. Data Gene number:___4__________ mRNA: AUG, AGA, AGU, AGG, AGA, AGC, AUA, AUC, UAG Protein:_The beatles are the best rock band. ______________________________________________________________________ -------------------------------------------------------------------------------------------------------------------- Gene number:_______5______ mRNA:_AUG, AUU, CAA, CAC, AUC, CAG, CCA, CAU, UAG Protein:__An old rubber band breaks when pulled. _____________________________________________________________________ -------------------------------------------------------------------------------------------------------------------- Gene number:_____6________ mRNA:AUG, CCC, CCG, AGA, AGC, CCU, UAG Protein:_ Biology is the best subject. ______________________________________________________________________ Analysis 1. Why is the making of mRNA called transcription and not replication? 2. Why is the making of mRNA called transcription and not translation? 3. Why is the making of protein called translation? 4. Identify the organelles where transcription and translation occur. Conclusion 1. Do you feel that you are able to decode the genetic code found in living organisms? Why or why not? 2. Do you think this lab should be done in groups of two, four, or individually? Why? 3. What was the easiest part of this lab? What was the most difficult part? Codon Chart for Proteins (sentences) DNA Cards 1. TAC,TTT,TTG,TTC,CAT,GTG,TAG,ATC
  2. 2. 2. TAC,TTT,TTG,TTA,ACG,TGC,ATC 3. TAC,ATT,TGG,TGA,TGT,ATC 4. TAC,TCT,TCA,TCC,TCT,TCG,TAT,TAG,ATC AUG AGA AGU AGG AGA AGC AUA AUC UAG 5. TAC,TAA,GTT,GTG,TAG,GTC,GGT,GTA,ATC AUG,AUU,CAA,CAC,AUC,CAG,CCA,CAU,UAG 6. TAC,GGG,GGC,TCT,TCG,GGA,ATC AUG, CCC, CCG, AGA, AGC, CCU, UAG 7. TAC,GCT,GCG,GCC,GCA,ATC 8. TAC,GAT,GAG,TAT,CTA,GAC,GAA,ATC 9. TAC,ATT,TCC,CTT,CTG,CTC,ATC 10. TAC,GGG,GGC,CGT,CGG,CGC,ATC 11. TAC,CGA,GGC,TCT,CCT,CCG,TCT,CCC,ATC 12. TAC,TTT,CCA,TTC,CAT,CAG,ATC 13. TAC,TTT,CAC,TTC,CAA,ATC 14. TAC,ATT,TCC,CTT,ATG,ATA,AGT,ATC 15. TAC,ATT,AGG,AGC,AGA,GCC,GCA,ATC 16. TAC,TAT,CTA,GAC,GAA,GGC,TCT,TCG,ATC 17. TAC,GGG,GGC,CTT,ACT,ACG,ATC 18. TAC,ACC,CAT,ACA,GCC,GCA,ATC 19. TAC,ATT,GGC,TCT,AAG,AAC,AAA,ATC 20. TAC,AAT,AGG,AGC,ACC,AAC,AAA,ATC

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