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Protein Protein Document Transcript

  • Protein Synthesis LabObjectivesThe objective of this lab is to understand how genes are expressed.Materials • 20 DNA Template Cards: these will be kept on the supply table at all times. After all, DNA cannot leave the nucleus. • Codon chart for sentencesProcedure 1. Your group will be assigned a number 2. Go to the table, with the DNA template cards 3. Find the card with your number 4. While staying in the nucleus (the table with the cards), transcribe the mRNA, from your DNA card, onto your lab report. 5. Return to your table. 6. Using the codon chart, translate your protein from the mRNA 7. Get another number from your teacher to make another protein.DataGene number:_____________mRNA:_______________________________________________________________________Protein:_______________________________________________________________________--------------------------------------------------------------------------------------------------------------------Gene number:_____________mRNA:_______________________________________________________________________Protein:_______________________________________________________________________--------------------------------------------------------------------------------------------------------------------Gene number:_____________mRNA:_______________________________________________________________________Protein:_______________________________________________________________________
  • Analysis1. Why is the making of mRNA called transcription and not replication?2. Why is the making of mRNA called transcription and not translation?3. Why is the making of protein called translation?4. Identify the organelles where transcription and translation occur.Conclusion1. Do you feel that you are able to decode the genetic code found in living organisms? Why orwhy not?2. Do you think this lab should be done in groups of two, four, or individually? Why?3. What was the easiest part of this lab? What was the most difficult part?
  • Codon Chart for Proteins (sentences)
  • DNA Cards1. TAC,TTT,TTG,TTC,CAT,GTG,TAG,ATC2. TAC,TTT,TTG,TTA,ACG,TGC,ATC3. TAC,ATT,TGG,TGA,TGT,ATC4. TAC,TCT,TCA,TCC,TCT,TCG,TAT,TAG,ATC5. TAC,TAA,GTT,GTG,TAG,GTC,GGT,GTA,ATC6. TAC,GGG,GGC,TCT,TCG,GGA,ATC7. TAC,GCT,GCG,GCC,GCA,ATC8. TAC,GAT,GAG,TAT,CTA,GAC,GAA,ATC9. TAC,ATT,TCC,CTT,CTG,CTC,ATC10. TAC,GGG,GGC,CGT,CGG,CGC,ATC11. TAC,CGA,GGC,TCT,CCT,CCG,TCT,CCC,ATC12. TAC,TTT,CCA,TTC,CAT,CAG,ATC13. TAC,TTT,CAC,TTC,CAA,ATC14. TAC,ATT,TCC,CTT,ATG,ATA,AGT,ATC15. TAC,ATT,AGG,AGC,AGA,GCC,GCA,ATC16. TAC,TAT,CTA,GAC,GAA,GGC,TCT,TCG,ATC17. TAC,GGG,GGC,CTT,ACT,ACG,ATC18. TAC,ACC,CAT,ACA,GCC,GCA,ATC19. TAC,ATT,GGC,TCT,AAG,AAC,AAA,ATC20. TAC,AAT,AGG,AGC,ACC,AAC,AAA,ATC