DEL MAR COLLEGEDepartment of Natural SciencesLABORATORY SAFETY PROCEDURES & GUIDELINES A Pre-Lab Course 24-Hour Emergency Number: DMC Security Office 361 / 698-1199
INSTRUCTIONS • Study the information in this module. • Take the quiz following this module. • You must score 100% on this quiz to stay registered in the class.
TABLE OF CONTENTS Topic Module General Rules and Regulations 1 Chemical Safety 2 Biohazard Safety 3 Emergency Equipment & Procedures 4 Field Trip Safety 5
MODULE 3BIOHAZARD SAFETY
BIOHAZARDS BIOHAZARDS INCLUDE: Bacteria Fungi Viruses Parasites any bodily fluidsand anything that comes in contact with them.
BLOODBORNE PATHOGENS (BBPs) PATHOGENS are agents that cause disease. BLOODBORNE PATHOGENS are microorganisms (bacteria, viruses) that may be transmitted through blood or other potentially infectious materials such as bodily fluids (blood, saliva, urine, etc.) or tissues. Diseases of concern include Hepatitis viruses, and HIV (AIDS virus). The possibility of contracting one of these diseases should be a serious concern for everyone. Care should be taken to prevent contamination.
BLOODBORNE PATHOGENTRANSMISSIONYou can be contaminated with a bloodborne pathogen: • through a cut or puncture from a contaminated sharp object • when you touch a contaminated surface and then touch your eyes, nose, mouth, open wounds, or inflamed skin
CONTROLLING EXPOSURE TO BBPsExercise Universal Precautions!● Treat all blood and bodily fluids as if they were known to be infectious (Universal Precautions).● Avoid touching your face, eyes and nose with your gloves● Wash hands before leaving the laboratory.● Follow proper work practices● Use safety equipment such as Fume Hoods● Wear PPE as required by Laboratory Supervisor● Follow proper waste disposal practices
BIOHAZARD DISPOSAL• Specific waste disposal procedures depend on the type of waste involved.• Follow the disposal instructions as provided by the Laboratory Supervisor.• These photos are examples of containers that you may see in the laboratory.
BIOHAZARD DISPOSALBiohazard Contaminated Soft ItemsItems contaminated with biohazards mustbe placed in a “Biohazardous Waste”container. These items include: • Disposable pipettes • Gloves • Plastic petri dishes • Microcentrifuge tubes, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSALSharps Disposal● Items identified as “Sharps” will be deposited in a rigid “Sharps” container. These items include: ● Used scalpel blades ● Hypodermic Needles ● Razor blades ● Contaminated glass slides● Do not recap hypodermic needles prior to disposing in a “Sharps” container. Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSALNon-biohazard ItemsItems NOT contaminated with biohazards and can NOTbe placed in regular trash container must be placed indesignated waste containers. These items include: ● Broken glass and microscope slides ● Autoclavable items such as: o Disposable pipettes Broken o Plastic petri dishes Glass o Microcentrifuge tubes, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSALNon-biohazard Dissection ItemsSolid and liquid waste items generated during adissection must be disposed of in separate designatedwaste containers. Liquids Solids Only Only Follow the disposal instructions as provided by the Laboratory Supervisor.
BIOHAZARD DISPOSALLaboratory Trash ItemsItems NOT contaminated with biohazards can be placedin the regular trash container. These items include: ● Used gloves ● Paper towels, etc. Follow the disposal instructions as provided by the Laboratory Supervisor.
REMINDER Please complete the quiz for Module 3 before continuing.