1. DEL MAR COLLEGEDepartment of Natural Sciences LABORATORY SAFETY PROCEDURES & GUIDELINES A Pre-Lab Course 24-Hour Emergency Number: DMC Security Office 361 / 698-1199
2. INSTRUCTIONS • Study the information in this module. • Take the quiz following this module. • You must score 100% on this quiz to stay registered in the class. You may take the quiz as many times as needed to achieve 100%.
3. TABLE OF CONTENTS Topic Module General Rules and Regulations 1 Chemical Safety 2 Biohazard Safety 3 Emergency Equipment & Procedures 4 Field Trip Safety 5
4. MODULE 1 GENERAL LABORATORY SAFETY PROCEDURES
5. BASIC SAFETY PRINCIPLES Practice safety for yourself Practice safety for others Learn the hazards Emergency? Call DMC Security Office at 698-1199 Report hazardous conditions to your Instructor
6. ACCESS TO LABORATORY SAFETYINFORMATION• Your Instructor will provide you with Safety Information.• You will receive a Laboratory specific Chemical Hygiene Plan Brochure which will give you specific safety information depending on your lab course.• Each Laboratory includes an Emergency Reference Poster which outlines emergency procedures.
7. ENFORCEMENTAny person NOT COMPLYING with the LaboratorySafety Program WILL be asked to leave thelaboratory and MAY be dismissed from the course.It is the obligation of every student to comply withthese laboratory safety procedures.
8. MINIMUM REQUIRED APPARELo Lab coats are required in some labs (button up the coat).o Ankle-length pants are required in all labs.o Only closed-toe and closed-heel shoes. No flip flops, sandals, etc…o Do NOT wear loose clothing in the lab (scarves, hats…).
9. EYE PROTECTIONProtect your eyes from flying objects, dust particles,& splashes. • Splash-resistant Safety Goggles must be worn during all labs unless instructed otherwise. Goggles must be stamped with ANSI Z87. • Do NOT handle contact lenses in lab. NOTE: Safety Glasses will not be allowed in the Laboratory.
10. HAND PROTECTIONGloves protect your hands from chemical andbiohazard exposures, therefore latex or nitrilelaboratory gloves are required when working withthese substances.o Inspect gloves before wearing them.o Remove and replace torn/dirty gloves.
11. SAFETY: IT’S YOUR RESPONSIBILITY!!o Read the experimental procedure before you enter the lab.o Note any potential risks.o Think before you touch. Do not touch hot glassware or hardware.o Take special care when working with strong acids or bases; contact can cause severe burns.o Inform your Instructor of any allergies or medical conditions.
12. GENERAL BEHAVIORFood, drinks or gum are NOT Do not apply cosmetics in allowed in any laboratory the laboratory No horseplay! (pushing, shoving, pranks, etc...)
13. BASIC POINTSo NEVER pipette by mouth.o No unauthorized or unsupervised experiments are allowed.o All work is to be completed during regularly scheduled laboratory periods.o Contact your instructor regarding the clean-up of spills or broken glass.o Read the label on all containers BEFORE and AFTER you remove the contents.
14. SAFETY HABITSo Never leave a fire unattended, always turn off Bunsen burners.o Be aware of Bunsen burners emitting invisible flames.o Immediately report ALL accidents, injuries, and spills to your Instructor.
15. HOUSEKEEPINGo Keep all work areas and aisle ways clean and clutter free.o Wipe down benches regularly.o Stow personal items in designated locations away from emergency equipment and exits. • Inside cabinets, along side walls and beneath desks or as instructed by your Laboratory Supervisor.
16. MICROSCOPE CAREo Hold the scope with both hands.o Don’t swing them around – you may hit someone.o Clean lenses and slides with lens paper only. o Store with scanning objective (small lens) in position & stage lowered.
17. ELECTRICAL HAZARDSProtect yourself from electrical hazards: o Do not use equipment with damaged or frayed cords – Report them immediately.o Keep electrical equipment away from wet areas.o In case of electrocution do not touch the victim.o Activate the emergency shut-off button.o In case of power outage remain calm and follow the instructions of your Laboratory Supervisor.
18. WASTE DISPOSAL Non-hazardous Wastes:● Non-hazardous wastes generated in the laboratory may be disposed of in the trash container.● Any recyclable items used in the laboratory should be placed in a recycle container.● Follow the instructions of your Laboratory Supervisor.
19. WASTE DISPOSALHazardous Wastes:Most of the chemical wastegenerated in the laboratorywill be Hazardous Waste andmust be disposed ofproperly. ● Pour waste laboratory chemicals into properly labeled containers. ● Use a funnel to avoid spilling chemicals. ● Chemical disposal in the sink is normally PROHIBITED. Follow the instructions of your Laboratory Supervisor.
20. WASTE DISPOSAL Biohazardous Wastes:Biohazardous Waste can not beplaced in the trash container andmust be disposed of properly.● Biohazardous Waste containers must be lined with a RED bag.● Trash and other non biohazardous waste must not be placed in the container.● Items identified as “Sharps” must not be placed in the biohazardous waste box.● Follow the instructions of your Laboratory Supervisor.
21. WASTE DISPOSALBroken Glass Items:● Dispose of all broken glass in the “Broken Glass” container.● Broken glass items MUST NOT be placed in the trash container.● Follow the instructions of your Laboratory Supervisor.
22. LAB CLEAN-UPo Return supplies to their proper place.o Clean & disinfect work area BEFORE leaving the lab.o Wash glassware with soap and water then rinse with distilled-water.o Wash hands after removing gloves and before leaving the lab.
23. BEFORE LEAVING● Turn off all: o Gas, water, vacuum, and compressed air o Power supplies o Heating apparatus● Dispose of waste properly● Report defective equipment or supplies● Wash your hands!!!
24. REMINDER Please complete the quiz for Module 1 before continuing.