View stunning SlideShares in full-screen with the new iOS app!Introducing SlideShare for AndroidExplore all your favorite topics in the SlideShare appGet the SlideShare app to Save for Later — even offline
View stunning SlideShares in full-screen with the new Android app!View stunning SlideShares in full-screen with the new iOS app!
FibromyalgiaHow Does The Pain Start ? Jeremy Ríos Segarra Dr. Edwin Vazquez Biol 1300 University of Puerto Rico at Cayey
Fibromyalgia Fibromyalgia is a disorder characterized by widespread musculoskeletal pain accompanied by fatigue, sleep, memory and mood issues. Researchers believe that fibromyalgia amplifies painful sensations by affecting the way your brain processes pain signals.
Fibromyalgia It has been called a “stress-related disorder” due to the fact that its symptoms starts and/or worsen occur in the context of stressor events.
Objective Determine if the ApolipoproteinE (APOE) is the cause of the susceptibility that the siblings are affected with.
Hypothesis The APOE is the factor that siblings from parents with Fibromyalgia are inheriting.
Specific aims Gather blood samples with and without Fibromyalgia. Amplified directly from whole blood using Finnzymes ́ Phusion® Blood Direct PCR Kit. Digest with restriction enzymes. Run agarose gel electrophoresis.
Methodology From 20 to 40 adult female individuals age 47 to 59, with a clinical diagnosis of fibromyalgia. Control sample consists from 20 to 40 adult female individuals from age 47 to 59
Methodology Blood samples from both groups will be extracted and we will be using PCR/RFLP technique to identify the APOE gene.
PCR/RFLP A Restriction Fragment Length is a variation in the DNA sequence of a genome which can be detected by a laboratory technique known as gel electrophoresis. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.
Expected Results The APOE is presented on the siblings This gene is the one making the sibling susceptible to the Fibromyalgia.
References Mayo Clinic staff, Fibromyalgia, Mayo Foundation for Medical Education and Research (MFMER), Jan. 22, 2011. http://www.mayoclinic.com/health/fibromyalgia/DS00079 William C. Shiel Jr., Fibromyalgia,MedicineNet, Inc,June 30, 2011. http://www.medicinenet.com/fibromyalgia/article.htm Connie Luedtke, R.N., Is fibromyalgia hereditary?,April 9, 2011. http://www.mayoclinic.com/health/is-fibromyalgia-hereditary/AN01962 Fibromyalgia, A.D.A.M, February 14, 2011. http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001463/ Fibromyalgia, Ideal Health Services Pty Ltd., http://www.genhealth.com/Info/Articles/fibromyalgia.htm PCR-RFLP directly from blood using Phusion® Blood Direct PCR KitPCR-RFLP directly from blood using Phusion® Blood Direct PCR Kit,ArktikTM and Phusion®, http://www.finnzymes.com/pdf/pcr_rflp_phusion_blood_app_protocol.pdf