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  • 1. FibromyalgiaHow Does The Pain Start ?
    Jeremy Ríos Segarra
    Dr. Edwin Vazquez
    Biol 1300
    University of Puerto Rico at Cayey
  • 2. Fibromyalgia
    Fibromyalgia is a disorder characterized by widespread musculoskeletal pain accompanied by fatigue, sleep, memory and mood issues. Researchers believe that fibromyalgia amplifies painful sensations by affecting the way your brain processes pain signals.
  • 3. Fibromyalgia
    It has been called a “stress-related disorder” due to the fact that its symptoms starts and/or worsen occur in the context of stressor events.
  • 4. Objective
    Determine if the ApolipoproteinE (APOE) is the cause of the susceptibility that the siblings are affected with.
  • 5. Hypothesis
    The APOE is the factor that siblings from parents with Fibromyalgia are inheriting.
  • 6. Specific aims
    Gather blood samples with and without Fibromyalgia.
    Amplified directly from whole blood using Finnzymes ́ Phusion® Blood Direct PCR Kit.
    Digest with restriction enzymes.
    Run agarose gel electrophoresis.
  • 7. Methodology
    From 20 to 40 adult female individuals age 47 to 59, with a clinical diagnosis of fibromyalgia.
    Control sample consists from 20 to 40 adult female individuals from age 47 to 59
  • 8. Methodology
    Blood samples from both groups will be extracted and we will be using PCR/RFLP technique to identify the APOE gene.
  • 9. PCR/RFLP
    A Restriction Fragment Length is a variation in the DNA sequence of a genome which can be detected by a laboratory technique known as gel electrophoresis. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.
  • 10. Expected Results
    The APOE is presented on the siblings
    This gene is the one making the sibling susceptible to the Fibromyalgia.
  • 11. References
    Mayo Clinic staff, Fibromyalgia, Mayo Foundation for Medical Education and Research (MFMER), Jan. 22, 2011. http://www.mayoclinic.com/health/fibromyalgia/DS00079
    William C. Shiel Jr., Fibromyalgia,MedicineNet, Inc,June 30, 2011. http://www.medicinenet.com/fibromyalgia/article.htm
    Connie Luedtke, R.N., Is fibromyalgia hereditary?,April 9, 2011. http://www.mayoclinic.com/health/is-fibromyalgia-hereditary/AN01962
    Fibromyalgia, A.D.A.M, February 14, 2011. http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001463/
    Fibromyalgia,  Ideal Health Services Pty Ltd., http://www.genhealth.com/Info/Articles/fibromyalgia.htm
    PCR-RFLP directly from blood using Phusion® Blood Direct PCR KitPCR-RFLP directly from blood using Phusion® Blood Direct PCR Kit,ArktikTM and Phusion®, http://www.finnzymes.com/pdf/pcr_rflp_phusion_blood_app_protocol.pdf