Bsl 3 Lab Evaluation Boston


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This presentation was an introduction to construction and commissioning of BioSafety Level 3 labs presented to New England Clients.

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  • Jerry Bauers
  • Biosafety in Microbiological and Biomedical Laboratories by the CDC AALAS – American Association for Laboratory Animal Science Laboratory biosafety manual published by the World Health Organization Laboratory Biosafety Guidelines published by Public Health Agency of Canada Applied Biosafety: Journal of the American Biological Safety Association
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Jerry Bauers
  • Bsl 3 Lab Evaluation Boston

    1. 1. Practical Implications BMBL Guidelines BSL-3 Laboratory Boston, Massachusetts January, 2010 Sebesta Blomberg Associates Jerry Bauers, PE National Director of Commissioning
    2. 2. Codes and Standards <ul><li>Biosafety in Microbiological and Biomedical Laboratories, 5 th Edition , 2007, CDC & NIH </li></ul><ul><li>Design Requirements Manual , National Institutes of Health, 2008 </li></ul><ul><li>Laboratory Biosafety Manual , World Health Organization, Third Edition, 2004 </li></ul>
    3. 3. Biosafety Guides and Publications
    4. 4. BMBL Cornerstones <ul><li>Code of Practice for Safe Handling of Biological Agents </li></ul><ul><ul><li>Risk Assessment </li></ul></ul><ul><ul><li>Containment </li></ul></ul>
    5. 5. BMBL Cornerstones <ul><ul><li>Risk Assessment </li></ul></ul><ul><ul><li>Characteristics of Infectious Agents </li></ul></ul><ul><ul><li>Method of Exposure/Transmission </li></ul></ul><ul><ul><li>Risk of Infectious Incident </li></ul></ul><ul><ul><li>Severity of Resulting Infection </li></ul></ul>
    6. 6. BMBL Cornerstones <ul><ul><li>Containment </li></ul></ul><ul><ul><li>Safe Laboratory Practices </li></ul></ul><ul><ul><li>Safety Equipment </li></ul></ul><ul><ul><li>Facility Safeguards </li></ul></ul>
    7. 7. <ul><li>Biosafety Level 3 is applicable to clinical, diagnostic, teaching, research, or production facilities in which work is done with indigenous or exotic agents with a potential for respiratory transmission , and which may cause serious and potentially lethal infection . </li></ul>BSL-3 Labs CDC Std 5 th Ed
    8. 8. <ul><li>All procedures involving the manipulation of infectious materials must be conducted within biological safety cabinets or other physical containment devices or by personnel wearing personal protective equipment . </li></ul>BSL-3 Labs CDC Std 5 th Ed
    9. 9. <ul><ul><ul><li>Access to the laboratory is limited or restricted at the discretion of the laboratory director when experiments are in progress. </li></ul></ul></ul><ul><ul><ul><li>Work surfaces are decontaminated at least once a day and after any spill of viable material. </li></ul></ul></ul><ul><ul><ul><li>All cultures, stocks, and other regulated wastes are decontaminated before disposal by an approved decontamination method, such as autoclaving . </li></ul></ul></ul>BSL-3 Labs CDC Std 5 th Ed
    10. 10. <ul><ul><ul><li>Protective laboratory clothing such as solid-front or wraparound gowns, scrub suits, or coveralls are worn by workers when in the laboratory. Protective clothing is not worn outside the laboratory . Reusable clothing is decontaminated before being laundered. </li></ul></ul></ul>BSL-3 Labs CDC Std 5 th Ed
    11. 11. <ul><ul><ul><li>The laboratory is separated from areas that are open to unrestricted traffic flow within the building, and access to the laboratory is restricted . </li></ul></ul></ul><ul><ul><ul><li>Passage through a series of two self-closing doors is the basic requirement for entry into the laboratory from access corridors. </li></ul></ul></ul><ul><ul><ul><li>Doors are lockable (see Appendix F). A clothes change room may be included in the passage way. </li></ul></ul></ul>BSL-3 Labs CDC Std 5 th Ed
    12. 12. BSL-3 Labs CDC Std 5 th Ed <ul><ul><ul><li>The interior surfaces of walls, floors, and ceilings of areas where BSL-3 agents are handled are constructed for easy cleaning and decontamination . </li></ul></ul></ul><ul><ul><ul><li>Bench tops are impervious to water and are resistant to moderate heat and the organic solvents, acids, alkalis, and those chemicals used to decontaminate the work surfaces and equipment. </li></ul></ul></ul>
    13. 13. BSL-3 Labs CDC Std 5 th Ed <ul><ul><ul><li>A method for decontaminating all laboratory wastes is available in the facility and utilized, preferably within the laboratory (i.e., autoclave, chemical disinfection, incineration, or other approved decontamination method). </li></ul></ul></ul><ul><ul><ul><li>Biological safety cabinets must be installed so that fluctuations in room air supply and exhaust do not interfere with proper operations. </li></ul></ul></ul>
    14. 14. BSL-3 Labs CDC Std 5 th Ed <ul><li>A ducted air ventilation system is required. This system must provide sustained directional airflow which draws air into the laboratory from &quot;clean&quot; areas and toward &quot;contaminated“ areas. </li></ul><ul><li>A visual monitoring device which confirms directional air flow must be provided…. </li></ul><ul><li>The laboratory exhaust air must not recirculate to any other area of the building. </li></ul><ul><li>… building exhaust air should be dispersed away from occupied areas and from building air intake locations or the exhaust air must be HEPA filtered. </li></ul><ul><li>An eyewash station is readily available inside the laboratory. </li></ul>
    15. 15. BSL-3 Labs CDC Std 5 th Ed <ul><ul><ul><li>The BSL-3 facility design, operational parameters, and procedures must be verified and documented prior to operation . Facilities must be re-verified and documented at least annually. </li></ul></ul></ul>
    16. 16. Web Site References <ul><li>AALAS – </li></ul><ul><li>ARS – </li></ul><ul><li>CDC – </li></ul><ul><li>Office of Biotechnology Activities – </li></ul><ul><li>DHHS – </li></ul><ul><li>NIH – </li></ul><ul><li>USDA APHIS – / </li></ul>