Chapter 18b - Prokaryotic Gene Regulation

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Chapter 18b - Prokaryotic Gene Regulation

  1. 1. Control of Prokaryotic (Bacterial) GenesAP Biology 2007-2008
  2. 2. Bacterial metabolism  Bacteria need to respond quickly to changes in their environment  if they have enough of a product, need to stop production  why? waste of energy to produce more STOP  how? stop production of enzymes for synthesis  if they find new food/energy source, need to utilize it quickly  why? metabolism, growth, reproduction GO  how? start production of enzymes for digestionAP Biology
  3. 3. Remember Regulating Metabolism?  Feedback inhibition - = inhibition  product acts as an allosteric inhibitor of 1st enzyme in tryptophan pathway  but this is wasteful production of enzymes - Oh, I remember this from our Metabolism Unit!AP Biology
  4. 4. Different way to Regulate Metabolism  Gene regulation - = inhibition  instead of blocking enzyme function, block transcription of genes for all enzymes in tryptophan pathway  saves energy by not wasting it on - unnecessary - protein synthesis Now, that’s a good idea from aAP Biology lowly bacterium!
  5. 5. Gene regulation in bacteria  Cells vary amount of specific enzymes by regulating gene transcription  turn genes on or turn genes off turn genes OFF example  if bacterium has enough tryptophan then it STOP doesn’t need to make enzymes used to build tryptophan  turn genes ON example if bacterium encounters new sugar (energy GO source), like lactose, then it needs to start making enzymes used to digest lactoseAP Biology
  6. 6. Bacteria group genes together  Operon  genes grouped together with related functions  example: all enzymes in a metabolic pathway  promoter = RNA polymerase binding site  single promoter controls transcription of all genes in operon  transcribed as one unit & a single mRNA is made  operator = DNA binding site of repressor proteinAP Biology
  7. 7. So how can these genes be turned off?  Repressor protein  binds to DNA at operator site  blocking RNA polymerase  blocks transcriptionAP Biology
  8. 8. Operon model Operon: operator, promoter & genes they control RNApolymerase serve as a model for gene regulation RNA TATA repressor polymerase gene1 gene2 gene3 gene4 DNA mRNA 1 2 3 4 enzyme1 enzyme2 enzyme3 enzyme4 promoter operatorRepressor protein turns off gene by repressor = repressor proteinblocking RNA polymerase binding site. AP Biology
  9. 9. Repressible operon: tryptophan Synthesis pathway model RNA When excess tryptophan ispolymerase present, it binds to tryp repressor protein & triggers repressor to bind to DNA RNA blocks (represses) transcription  TATA repressor trp polymerase gene1 gene2 gene3 gene4 DNA mRNA 1 2 3 4 trp trp trp trp enzyme1 enzyme2 enzyme3 enzyme4 promoter operator trp trp repressor repressor protein trp trp trp tryptophan trp conformational change in tryptophan – repressor protein AP Biology repressor protein! trp repressor complex
  10. 10. Tryptophan operon What happens when tryptophan is present? Don’t need to make tryptophan-building enzymes TryptophanAP Biology is allosteric regulator of repressor protein
  11. 11. Inducible operon: lactose lac lac Digestive pathway model lac lac RNA When lactose is present, binds topolymerase lac lac repressor protein & triggers lac repressor to release DNA lac  induces transcription RNA TATA repressor lac polymerase gene1 gene2 gene3 gene4 DNA mRNA 1 2 3 4 enzyme1 enzyme2 enzyme3 enzyme4 promoter operator repressor repressor protein lac lactose conformational change in lactose – repressor protein AP Biology lac repressor repressor protein! complex
  12. 12. Lactose operon What happens when lactose is present? Need to make lactose-digesting enzymes Lactose is allosteric regulator of repressor proteinAP Biology
  13. 13. 1961 | 1965 Jacob & Monod: lac Operon  Francois Jacob & Jacques Monod  first to describe operon system  coined the phrase “operon”AP Biology Jacques Monod Francois Jacob
  14. 14. Operon summary  Repressible operon  usually functions in anabolic pathways  synthesizing end products  when end product is present in excess, cell allocates resources to other uses  Inducible operon  usually functions in catabolic pathways,  digesting nutrients to simpler molecules  produce enzymes only when nutrient is available  cell avoids making proteins that have nothing to do, cell allocates resources to other usesAP Biology
  15. 15. Don’t be repressed! How can I induce you to ask Questions?AP Biology 2007-2008

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