By learning, you will teach. By teaching you will understand. -A Latin Proverb<br />BIOTECHNOLOGY(To Teachers & Students of Karnataka SSLC)<br />DR. M. JAYAKARA BHANDARY<br />DEPT. OF BOTANY & BIOTECHNOLOGY<br />GOVERNMENT SCIENCE COLLEGE, KARWAR<br />
Definition<br />Applications of biological systems / processes / organisms to manufacturing (Products) or serviceindustries (Services).<br />
GENETIC ENGINEERING<br />Changing genetic material of an organism by transferring genes from other organisms<br />Creating organisms with new genes/ characters – Genetically modified Organisms (GMO’s) or transgenic Organisms.<br />Addition or Modification of characters of organisms<br />
Recombinant DNA technology – Joining or combining genes from two or more organisms in the laboratory.<br />
DNA FINGERPRINTING<br />Technique which helps in the establishment of identity of a person or organism based on uniqueness of DNA sequence. <br />Every person’s DNA sequence is different from all others, like finger print or signatures.<br />
Techniques…<br />. Collection of DNA, cutting into pieces, Separation of pieces by Gel Electrophoresis, Processing of separated DNA bands which appear like ‘Bar Codes’.<br />
DNA fingerprint of a person <br />Application : Detection of criminals, solving parental disputes<br />
PLANT TISSUE CULTURE<br />Technique of growing isolated plant tissues or cells in an artificial medium under aseptic conditions.<br />Plant cells TOTIPOTENT. Each cell can Multiply and develop in to new plants when cultured.<br />Growth medium: Nutrients, vitamins, hormones, agar<br />
USES OF PLANT TISSUE CULTURE<br />Micropropagation – large number of plants in small space<br />Disease free plant production<br />Somatic hybridisation/cybrid production- Pomato<br />Transgenic plant production – Nif gene transfer<br />Pollen culture – haploid plants – Parthenocarpy – Protection of pure charecters.<br />Shortening of Flowering Period – Rose?<br />
Cloning<br />Identicle/ exactly similar cells / organisms <br />Plants produced by cuttings/ vegetative propagation are genetically similar – in vivoclones (Hibiscus, Rose, etc.) <br />Tissue culture Plants – in vitro Clones<br />Animals from cell of a single parent – in vitro method<br />Dolly sheep – first animal clone produced by Ian Wilmut.<br />Cloning is possible in frogs, rats, monkeys<br />Xerox copies of parent animal (usually superior quality mother) <br />
Limitations of Biotechnology<br />Genetic engineered food may cause Health Problems (Bt cotton, corn , brinjal etc. have a toxic gene of bacteria)<br />Pest resistant Bt plants also kill useful insects like butter flies, bees along with disease causing pest insects – ecological imbalance.<br />Human cloning – Socially, Ethically Right?<br />
THANK YOU<br />I want to touch the FUTURE . Therefore, I have become a TEACHER. - Christa McAuliff<br />firstname.lastname@example.org<br />08382 – 222458 ®<br />
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