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Microscopes

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Microscopes Microscopes Presentation Transcript

  • Microscopy: The Science of the Microscope
  • The Invention of the Microscope
    • Renaissance invention (Mid 1600s)
    • Credited for invention: Anton Van Leeuenhoek
    • Constructed simple curved glass lenses in combination
  • Improvement of the Microscope
    • Robert Hooke
    • English biologist who discovered cells
    • Increased magnification with improved lenses
  • Modern Compound Light Microscopes
    Uses 2 lenses in combination to magnify an image
    Can view objects too small to be seen with unaided eye
    Object must be thin enough for light to pass through
    Can view living things
    Typical magnification 100x to 1000x
    See your packet for a detailed discussion of:
    • Parts and their functions
    • Proper use and handling
    • Procedures for making a wet mount
  • Pushing the Limits: Electron Microscopes
    • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light
    • Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur
    • Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light.
    • Electron microscopes were developed in the 1930s
  • Electron Microscopes
    • Uses a beam of electrons to view the specimen (not light)
    • Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope
    • Magnifies up to 200,000x magnification
  • Scanning Electron Microscope
  • Scanning Electron Microscope or SEM
    Bounces electrons off the surface of the object
    Produces a 3 dimensional image of the object
  • Red Blood Cells
  • Blood Clot
  • Nerve Cells
  • Taste Bud
  • Sperm on Surface of Human Egg
  • The Split End of a Human Hair
  • Tooth Plaque
  • Transmission Electron Microscope
  • Transmission Electron Microscope
    Electrons pass through the object forming a one dimensional picture
    Allows one to view the inside of an object (ex. internal structure of a cell)
  • filamentous bacteria from the gut of a termite
  • Sperm heads from a stick insect
  • Salmonella Bacteria
  • Stereoscope
    • Allows viewing of macroscopic objects with great detail
    • Does not require light to pass through object
    • Can view living things
    • Typical magnification of 10X to 30X
  • Choosing the Correct Microscope
  • Microscope Lab Skills Review
    Complete the microscope review activities on pages 41 and 43-44.