Microscopy: The Science of the Microscope
The Invention of the Microscope
• Renaissance invention (Mid 1600s)
• Credited for invention: Anton Van Leeuenhoek
• Const...
Improvement of the Microscope
• Robert Hooke
• English biologist who discovered cells
• Increased magnification with impro...
Modern Compound Light Microscopes
Uses 2 lenses in combination to
magnify an image
Can view objects too small to be seen
w...
Pushing the Limits: Electron Microscopes
• A light microscope cannot be used to distinguish objects that are smaller than ...
Electron Microscopes
• Uses a beam of electrons to view the specimen (not light)
• Specimen viewed must be prepared in a v...
Scanning Electron Microscope
Scanning Electron Microscope or SEM
• Bounces electrons off the
surface of the object
• Produces a 3 dimensional
image of ...
Red Blood Cells
Blood Clot
Nerve Cells
Taste Bud
Sperm on Surface
of Human Egg
The Split
End of a
Human Hair
Tooth Plaque
Transmission Electron Microscope
Transmission Electron Microscope
• Electrons pass
through the object
forming a one
dimensional picture
• Allows one to vie...
filamentous
bacteria from
the gut of a
termite
Sperm
heads
from a
stick
insect
Salmonella
Bacteria
Stereoscope
• Allows viewing of macroscopic objects with great detail
• Does not require light to pass through object
• Ca...
Choosing the Correct Microscope
Microscope Lab Skills Review
Complete the microscope review activities on pages 41 and 43-44.
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Microscopes

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Microscopes

  1. 1. Microscopy: The Science of the Microscope
  2. 2. The Invention of the Microscope • Renaissance invention (Mid 1600s) • Credited for invention: Anton Van Leeuenhoek • Constructed simple curved glass lenses in combination
  3. 3. Improvement of the Microscope • Robert Hooke • English biologist who discovered cells • Increased magnification with improved lenses
  4. 4. Modern Compound Light Microscopes Uses 2 lenses in combination to magnify an image Can view objects too small to be seen with unaided eye Object must be thin enough for light to pass through Can view living things Typical magnification 100x to 1000x See your packet for a detailed discussion of: • Parts and their functions • Proper use and handling • Procedures for making a wet mount
  5. 5. Pushing the Limits: Electron Microscopes • A light microscope cannot be used to distinguish objects that are smaller than half the wavelength of light • Any object with a diameter smaller than 0.275 micrometers will be invisible or, at best, show up as a blur • Electrons are speeded up in a vacuum until their wavelength is extremely short, only one hundred-thousandth that of white light. • Electron microscopes were developed in the 1930s
  6. 6. Electron Microscopes • Uses a beam of electrons to view the specimen (not light) • Specimen viewed must be prepared in a vacuum (no air molecules) therefore living things cannot be viewed using this type of scope • Magnifies up to 200,000x magnification
  7. 7. Scanning Electron Microscope
  8. 8. Scanning Electron Microscope or SEM • Bounces electrons off the surface of the object • Produces a 3 dimensional image of the object
  9. 9. Red Blood Cells
  10. 10. Blood Clot
  11. 11. Nerve Cells
  12. 12. Taste Bud
  13. 13. Sperm on Surface of Human Egg
  14. 14. The Split End of a Human Hair
  15. 15. Tooth Plaque
  16. 16. Transmission Electron Microscope
  17. 17. Transmission Electron Microscope • Electrons pass through the object forming a one dimensional picture • Allows one to view the inside of an object (ex. internal structure of a cell)
  18. 18. filamentous bacteria from the gut of a termite
  19. 19. Sperm heads from a stick insect
  20. 20. Salmonella Bacteria
  21. 21. Stereoscope • Allows viewing of macroscopic objects with great detail • Does not require light to pass through object • Can view living things • Typical magnification of 10X to 30X
  22. 22. Choosing the Correct Microscope
  23. 23. Microscope Lab Skills Review Complete the microscope review activities on pages 41 and 43-44.
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