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Dna replication transcription and translation

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  • 1. DNA replication transcription and translation
  • 2. Nucleotide Building block of DNA Three Parts Deoxyribose sugar Phosphate group Nitrogen base DNA Structure
  • 3. There are two classes of bases: Purines: Adenine and Guanine Pyrimidines: Cytosine and Thymine Nitrogenous Bases
  • 4. Many nucleotides are pieced together to make a DNA molecule DNA is a Polynucleotide
  • 5. A DNA chain consists of nucleotides joined by bonds between phosphate and sugar This makes up the sides of the DNA “ladder” DNA is a Polynucleotide
  • 6. • DNA consists of two polynucleotide chains wound around each other to form a double helix • Structure inferred from Rosalind Franklin’s famous X-Ray crystallography The Double Helix
  • 7. The two chains are held together by complementary base pairing Specific bonding between A and T bases and between G and C bases on the two strands Inferred from Erwin Chargaff’s research Chargaff’s Rules state that in any organisms genome the amount of A = T and the amount of C = G. The Double Helix
  • 8. The two DNA chains are held together by hydrogen bonds between nitrogen bases The two strands run in opposite directions, or are antiparallel Complementary Base Pairing
  • 9. Describes the flow of genetic information from DNA to RNA to Proteins DNA Replication Transcription Translation The Central Dogma of Molecular Biology
  • 10. DNA Replication is semi-conservative Each newly synthesized molecule contains 1 “parent template” strand and 1 new “daughter” strand DNA Replication
  • 11. Step 1: InitiationPreparing the DNA template Helicase unwinds DNA forming a “replication fork” Multiple replication forks along a DNA molecule create replication bubbles DNA Replication
  • 12. Step 2: Elongation Adding New Nucleotides DNA Replication
  • 13. RNA Primase adds a complimentary RNA primer to each template strand as a starting point for replication DNA Polymerase reads the template strand (3’ to 5’) and adds new complimentary nucleotides (5’ to 3’) DNA synthesized in the direction of the replication fork is called the leading strand Step 2: Elongation
  • 14. Step 2: Elongation
  • 15. DNA polymerase can only add new nucleotides in the 5’ to 3’ direction Because of the antiparallel nature of DNA, replication occurs in two directions An RNA primer is laid down on the other strand, and new nucleotides are added 5’ to 3’ moving away from the replication fork. This is the lagging strand and the segment of DNA produced is called an Okazaki fragment Step 2: Elongation The Lagging Strand
  • 16. Step 2: Elongation
  • 17. The DNA unwinds some more and the leading strand is extended by DNA polymerase adding more DNA nucleotides. Thus, the leading strand is synthesized continuously. Step 2: Elongation
  • 18. Step 2: Elongation
  • 19. On the top template strand, a new RNA primer is synthesized by primase near the replication fork DNA polymerase adds new DNA . This produces the second Okazaki fragment. Thus, the lagging strand is synthesized discontinously Step 2: Elongation
  • 20. Step 3: Termination
  • 21. A different type of DNA polymerase removes the RNA primer and replaces it with DNA DNA ligase joins the two Okazaki fragments with phosphodiester bonds to produce a continuous chain Each new DNA molecule is rewound by helicase. Each molecule is identical Step 3: Termination
  • 22. Leading Strand: 1 primer, 5’ to 3’ continuous Lagging Strand: multiple primers, 5’ to 3’ discontinuous In humans, DNA polymerase adds 50 nucleotides/second DNA polymerase can proofread its own work and does excision repair 1 in 10,000 bases are in error, after proofreading, rate of mutation is 1 in 10,000,000 Summary and Other Facts
  • 23. Check out these animations and reviews: DNA replication animation Meselson and Stahl experiment that showed DNA replication is semi-conservativ BioCoach Biosynthesis of DNA practice BioCoach adding new DNA practice
  • 24. RNA is an important type of nucleic acid that plays several roles in the production of protein RNA is necessary to carry the instructions of the DNA out of the nucleus and to the ribosomes RNA
  • 25. Messenger RNA carries the actual code that specifies the amino acid sequence in a polypeptide (protein) Making mRNA starts with a protein encoding gene on a template strand of DNA Protein Encoding Gene
  • 26. RNA Synthesis: Transcription
  • 27. RNA Polymerase binds to a promoter which is a region of bases that signals the beginning of a gene RNA Polymerase is bound to the TATA box of the promoter by transcription factors The double helix unwinds and is ready to be transcribed Transcription Step 1: Initiation
  • 28. RNA Polymerase moves along the protein encoding gene adding new RNA nucleotides in the 5’ to 3’ direction and complimentary to the DNA template Works at up to 60 nucleotides/second Transcription Step 2: Elongation
  • 29. RNA Polymerase reaches the terminator region of the protein encoding gene All the enzymes and factors are released The product of these 3 steps is called immature or pre-mRNA Transcription Step 3: Termination
  • 30. Check out these animations and reviews: Transcription animation Transcription BioCoach practice Transcription self-quiz RNA Synthesis: Transcription
  • 31. The language of nucleic acids in translated into the language of proteins Nucleic acids have a 4 letter language Proteins have a 20 letter language Translation
  • 32. The Genetic Code If 3 RNA bases code for 1 amino acid, RNA could code for 43 = 64 amino acids. More than enough coding capacity for 20 amino acids Code is redundant for most amino acids
  • 33. The “Players” Messenger RNA (mRNA) Ribosomes Transfer RNA (tRNA) Amino Acids
  • 34. Messenger RNA (mRNA) Synthesized in Transcription Composed of Codons Codons are 3-base sequences of mRNA
  • 35. Ribosomes Made of rRNA and protein 2 subunits (large and small) form a 3D groove 2 major sites: P site---holds the growing polypeptide A site---new amino acids enter here
  • 36. Transfer RNA (tRNA) Carries amino acids to the ribosome 3 base anitcodon pairs with the mRNA codon During tRNA charging each tRNA picks up an amino acid from the cytoplasm
  • 37. There are 20 amino acids, each with a basic structure Amino acids are held together by peptide bonds Amino Acids
  • 38. 3 Steps: 1) Initiation 2) Elongation 3) Termination Translation
  • 39. Step 1: Initiation 5’ G-cap of mRNA binds to ribosome Start codon AUG and anticodon with Methionine bind a P site A site is open and ready to receive new tRNAs
  • 40. Step 2: Elongation (Adding New Amino Acids) Codon recognition Peptide bond formation Translocation: ribosome moves along mRNA, aminoacyl tRNA shifts from A site to P site
  • 41. Step 3: Termination A stop codon is reached UAA UAG UGA All parts release
  • 42. Normally, the genetic code is translated and the correct protein is formed from a long chain of amino acids. Translation of codons is dependent on the reading frame, or a grouping of codons in a gene transcript. AAU GCG GAC UAC GGC AAC GCC Translation Polypeptides and Mutation
  • 43. Mutations: Any change in the nucleotide sequence of DNA Mutations can involve large sections of chromosomes or single base pairs Mutations may or may not change the reading frame of a gene transcript Translation Polypeptides and Mutation
  • 44. Translation Polypeptides and Mutation Sickle cell “Normal” red blood cell
  • 45. Sickle Cell Anemia Normal Hemoglobin Sickle Cell Hemoglobin DNA GGA CTT GCA GGA CAT GCA mRNA CCU GAA CGU CCU GUA CGU A.A. PRO GLU ARG PRO VAL ARG Changes in one or a few bases is called a Point Mutation 2 Types: Substitution Insertion/Deletions Translation Polypeptides and Mutation
  • 46. Deletion or insertion mutations are most disruptive because they change the reading frame, causing a frame shift Substitution mutations have varied impact on amino acid sequences. Substitutions of 1st or 2nd base in codon almost always changes the amino acid Substitution of 3rd base in codon does not always change the amino acid Translation Polypeptides and Mutation
  • 47. What causes mutations? Errors in DNA Replication Errors in chromosome crossover in meiosis Mutagens Mutagens are physical or chemical factors that cause mutations UV Radiation, X-Rays Chemicals like DDT Translation Polypeptides and Mutation
  • 48. Many mutations are harmful and cause the organism to die or function incorrectly. Mutations that result in helping organism to survive are beneficial. (Peppered Moths) If mutations are present in gametes, they can be passed on to offspring. This is the driving force of Natural Selection. Translation Polypeptides and Mutation