Rhododendron cultures in the lab of Herman Losely & Son Nursery, Perry, OH
Amelanchier laevis transplants and field planting.
Micropropagatio n Advantages• From one to many propagules rapidly• Multiplication in controlled lab conditions• Continuous propagation year round• Potential for disease-free propagules
Conventional Propagation Advantages• Equipment costs minimal• Experience can substitute for technical expertise• Costs per unit are minimal• Potential for disease-free propagules• Specialized techniques for growth control (grafting onto dwarfing rootstocks)
Micropropagatio n Limitations• Equipment/facility intensive operation• Technical expertise in management positions• Protocols not optimized for all species• Liners may not fit industry standard• Propagules may be too expensive
Micropropagatio n Applications• Rapid build up of stock of new, superior variety• Elimination of endophytic disease• Better suited to morphology/growth characteristics (few offshoots/sprouts; date palms, ferns, nandinas)• Propagules have enhanced growth features (multibranched character; Ficus, Syngonium)
Shoot organogenesisAdventitious meristems differentiate on leaf, stem, orfloral tissue.
Begonia x chiemanta ‘Emma’ at various stages in micropropagation.
Somatic EmbryogenesisStimulation of callus or suspension cells to undergo adevelopmental pathway that mimics the development ofthe zygotic embryo.
SomaticHybridizationFusion of protoplasts fromdifferent genotypes to bringabout genetic recombination.
Somaclonal variationHeritable genetic variation that arises in plantsproduced through tissue culture.
ChimeraPlant composed of two genotypes growing adjacentto one another in all (or part) of the plant body.
Inflorescence cultureThe inflorescence of many plants is a modified branchingsystem. Vegetative meristems in the inflorescence may bestimulated to proliferate in vitro. bract axi lpeduncle flower bud