A SYNOPSIS ON “IMMUNOMODULATORY EFFECT OF ANDROGRAPHIS PANICULATA” SUPERVISED BY: PRESENTED BY:DR. FAISAL IMAM [HOD] MD. IRSHAD ALAM Roll NO.- 1120698507 DEPARTMENT OF PHARMACOLOGY TRANSLAM INSTITUTE OF PHARMACEUTICAL EDUCATION & RESEARCH MEERUT-250001
INTRODUCTIONAndrographis paniculata a member of the family ofAcanthaceae and commonly known as Kalmegh, is a herbused in traditional medicine.As an Ayurveda herb it is known as Kalmegh orKalamegha, meaning "dark cloud.AP is an up to 1 meter tall annual shrub with lanceolateleaves and small white to pink, hairy flowers.It grows inmoist deciduous forests and is also cultivated.
PLANT PROFILEBotanical Name Andrographis paniculataCommon Name KalmeghFamily AcanthaceaePart-Used Whole Plant
CHEMICAL COMPOSITION Boorsma first isolated AND from AP in 1896 Corter identified AND as a lactone in 1911 It is a colourless or light yellow crystal compoundwith a very bitter taste Deng et al (1982) reported there are four lactones in Andrographis paniculata
They Are(1) 14-deoxyandrographolide.(2) Andrographolide.(3) Neoandrographolide (A Non-bitter, CoglucosideDerivative of the major Constituent Andrographolide)And(4) 14-deoxy- 11,12-didehydroandrographolide.
Figure 1-Structure of Andrographolide
PHARMACOLOGICAL PROPERTIES Anti-diarrhoeal and intestinal effects Effect on upper respiratory infections Effect on inflammation and fever Cardiovascular activity. Hepatoprotective. Immunomodulatory activity
IMMUNOMODULATORY EFFECTS Andrographis paniculata is a powerful stimulant for the immunologial system, through both the antigen specific and non specific way.It optimizes the expression of the genes that codify the cytokines’ receptors, and the synthesis of the histocompatibility molecules, type I and II; in this way, it improves the interaction between the competent cells
AIMS AND OBJECTIVESAIM: To evaluate the immune-modulatory effects ofandrographis paniculata OBJECTIVEAuthentication of plantCollection of plant materialsShade dry of collected plantExtraction of plant materialToxicity studyPharmacological activity
PLAN OF WORKANIMALAlbino Mice : from registered animal house,Age : 12 weeksWeight : 20-25gmSex : femaleDiet : Conventional laboratory diet
ANIMAL(cont…)12 week old female Albino Mice.The animals will be maintained in specificpathogen free conditions.Housed with 12 hours of light and 12 hours ofdarkness in a controlled temperature (23±1°C)
COLLECTION OF PLANT MATERIALThe leaves of Andrographis paniculata at different stageof maturity will be collected & dry in shade for 7 daysfollowed by grinding the fine leaf powder sample will beused. REAGENTS AND CULTURE MEDIAA stock solution for AND will be prepared bydissolving AND in food grade ethanol at 5 mg/ml.
SAMPLING OF MICEMice will be anaesthetized intraperitoneally with a mixtureof 40μl ketamine and 40μl xylazine. A cardiac puncture(23G ¾ needle) will be performed to obtain the bloodsample, followed by cervical cordotomy to ensure rapidand painless euthanasia before collection of the organs.
SERUM COLLECTIONBlood will be collected from anaesthetized mice viacardiac puncture in a 23G3/4 needle attached to 1 mlsyringe . then added to serum collection tubes and clottedon ice for at least 30 mins. The blood will be centrifugedfor 2 mins at room temperature. Serum will betransferred to a microfuge tube and stored at -20°C.
Statistical AnalysisAll statistical analysis was conducted using SPSS12.0 for Windows™. The data was analysed usingone-sided, independent samples t-tests
REFERENCES:1. Abbas, A. K., Murphy, K. M. and Sher, A. (1996) Functional diversity of helper T lymphocytes. Nature, 383, 787-793.2 . Sheeja K, Shihab PK, Kuttan G. Antioxidant and inflammatory modulating activities of the plant Andrographis paniculata Nees, Immunopharmacol Immunotoxicol. 2006; 6(1):66-73.3. Fujita et al.,1984; Rajani e eparation of andrographolide and neoandrographolide from the leaves of Andrographis paniculatat al., 2000; Du et al., 2003
4. Akbarsha, M. A., Manivannan, B., Hamid, K. S. and Vijayan, B. (1990) Antifertilit effect of Andrographis paniculata (nees) in male albino mice. Indian Journal of Experimental Biology, 28, 421-426.5. Zhu and Liu.,1984; Kotesw Evaluations of immunomodulatory properties of ethanol extract of Andrographis paniculata or andrographolideara et al., 2004