Immunomodulatory
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md irshad alam m.pharm (pharmacology) from T.I.P.E.R MERRUT

md irshad alam m.pharm (pharmacology) from T.I.P.E.R MERRUT

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Immunomodulatory Presentation Transcript

  • 1. A SYNOPSIS ON “IMMUNOMODULATORY EFFECT OF ANDROGRAPHIS PANICULATA” SUPERVISED BY: PRESENTED BY:DR. FAISAL IMAM [HOD] MD. IRSHAD ALAM Roll NO.- 1120698507 DEPARTMENT OF PHARMACOLOGY TRANSLAM INSTITUTE OF PHARMACEUTICAL EDUCATION & RESEARCH MEERUT-250001
  • 2. INTRODUCTIONAndrographis paniculata a member of the family ofAcanthaceae and commonly known as Kalmegh, is a herbused in traditional medicine.As an Ayurveda herb it is known as Kalmegh orKalamegha, meaning "dark cloud.AP is an up to 1 meter tall annual shrub with lanceolateleaves and small white to pink, hairy flowers.It grows inmoist deciduous forests and is also cultivated.
  • 3. PLANT PROFILEBotanical Name Andrographis paniculataCommon Name KalmeghFamily AcanthaceaePart-Used Whole Plant
  • 4. Figure 1: Plant. Andrographis paniculata
  • 5. BOTANICAL CLASSIFICATIONKingdom: PlantaeDivision : AngiospermaeClass : DicotilydonaeFamily : AcanthaceaeGenus : AndrographisSpecies : paniculata Nees
  • 6. CHEMICAL COMPOSITION Boorsma first isolated AND from AP in 1896 Corter identified AND as a lactone in 1911 It is a colourless or light yellow crystal compoundwith a very bitter taste Deng et al (1982) reported there are four lactones in Andrographis paniculata
  • 7. They Are(1) 14-deoxyandrographolide.(2) Andrographolide.(3) Neoandrographolide (A Non-bitter, CoglucosideDerivative of the major Constituent Andrographolide)And(4) 14-deoxy- 11,12-didehydroandrographolide.
  • 8. Figure 1-Structure of Andrographolide
  • 9. PHARMACOLOGICAL PROPERTIES Anti-diarrhoeal and intestinal effects Effect on upper respiratory infections Effect on inflammation and fever Cardiovascular activity. Hepatoprotective. Immunomodulatory activity
  • 10. IMMUNOMODULATORY EFFECTS Andrographis paniculata is a powerful stimulant for the immunologial system, through both the antigen specific and non specific way.It optimizes the expression of the genes that codify the cytokines’ receptors, and the synthesis of the histocompatibility molecules, type I and II; in this way, it improves the interaction between the competent cells
  • 11. AIMS AND OBJECTIVESAIM: To evaluate the immune-modulatory effects ofandrographis paniculata OBJECTIVEAuthentication of plantCollection of plant materialsShade dry of collected plantExtraction of plant materialToxicity studyPharmacological activity
  • 12. PLAN OF WORKANIMALAlbino Mice : from registered animal house,Age : 12 weeksWeight : 20-25gmSex : femaleDiet : Conventional laboratory diet
  • 13. ANIMAL(cont…)12 week old female Albino Mice.The animals will be maintained in specificpathogen free conditions.Housed with 12 hours of light and 12 hours ofdarkness in a controlled temperature (23±1°C)
  • 14. COLLECTION OF PLANT MATERIALThe leaves of Andrographis paniculata at different stageof maturity will be collected & dry in shade for 7 daysfollowed by grinding the fine leaf powder sample will beused. REAGENTS AND CULTURE MEDIAA stock solution for AND will be prepared bydissolving AND in food grade ethanol at 5 mg/ml.
  • 15. SAMPLING OF MICEMice will be anaesthetized intraperitoneally with a mixtureof 40μl ketamine and 40μl xylazine. A cardiac puncture(23G ¾ needle) will be performed to obtain the bloodsample, followed by cervical cordotomy to ensure rapidand painless euthanasia before collection of the organs.
  • 16. SERUM COLLECTIONBlood will be collected from anaesthetized mice viacardiac puncture in a 23G3/4 needle attached to 1 mlsyringe . then added to serum collection tubes and clottedon ice for at least 30 mins. The blood will be centrifugedfor 2 mins at room temperature. Serum will betransferred to a microfuge tube and stored at -20°C.
  • 17. Statistical AnalysisAll statistical analysis was conducted using SPSS12.0 for Windows™. The data was analysed usingone-sided, independent samples t-tests
  • 18. REFERENCES:1. Abbas, A. K., Murphy, K. M. and Sher, A. (1996) Functional diversity of helper T lymphocytes. Nature, 383, 787-793.2 . Sheeja K, Shihab PK, Kuttan G. Antioxidant and inflammatory modulating activities of the plant Andrographis paniculata Nees, Immunopharmacol Immunotoxicol. 2006; 6(1):66-73.3. Fujita et al.,1984; Rajani e eparation of andrographolide and neoandrographolide from the leaves of Andrographis paniculatat al., 2000; Du et al., 2003
  • 19. 4. Akbarsha, M. A., Manivannan, B., Hamid, K. S. and Vijayan, B. (1990) Antifertilit effect of Andrographis paniculata (nees) in male albino mice. Indian Journal of Experimental Biology, 28, 421-426.5. Zhu and Liu.,1984; Kotesw Evaluations of immunomodulatory properties of ethanol extract of Andrographis paniculata or andrographolideara et al., 2004