DNA Extraction
http://www.slideshare.net/iqbal1313/dna-
extraction-25521910
MATERIALS
• Dried split peas
• Dishwashing detergent
• Isopropyl alcohol
• Meat tenderizer
• Small cup or beaker
• Spoon
Start with the peas
Blend the peas with water and a
little salt until you have “pea soup”
Why do we blend the peas?
• The blender separates the pea CELLS
from each other
Put the strained pea soup in a cup
Add a squirt of dishwashing
detergent
Let the mixture sit for 5
minutes
What does the detergent do?
• Each pea cell is
surrounded by a cell
membrane
• DNA is in the nucleus
of a cell, which is a...
The membranes must be
broken open in order to get the
DNA out of the cell
Detergent:
• Breaks apart membranes by attaching to the
lipids (fats) & proteins in the membranes
The cell and nuclear membranes have been
broken apart,
as well as all of the organelle membranes,
such as those around the...
Add a pinch of meat tenderizer to
the mixture
Why?
• Meat tenderizer is an ENZYME
– A protein that helps chemical reactions
happen more quickly
• The enzymes in meat te...
The DNA in the nucleus is
surrounded by proteins
The meat tenderizer cuts away
the proteins from the DNA
Stir very, very GENTLY!
Tilt your cup & slowly pour a layer
of alcohol on top of the pea soup
mixture
• DNA will float up into the alcohol from th...
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DNA Extraction

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DNA Extraction

  1. 1. DNA Extraction http://www.slideshare.net/iqbal1313/dna- extraction-25521910
  2. 2. MATERIALS • Dried split peas • Dishwashing detergent • Isopropyl alcohol • Meat tenderizer • Small cup or beaker • Spoon
  3. 3. Start with the peas
  4. 4. Blend the peas with water and a little salt until you have “pea soup”
  5. 5. Why do we blend the peas? • The blender separates the pea CELLS from each other
  6. 6. Put the strained pea soup in a cup Add a squirt of dishwashing detergent
  7. 7. Let the mixture sit for 5 minutes
  8. 8. What does the detergent do? • Each pea cell is surrounded by a cell membrane • DNA is in the nucleus of a cell, which is also surrounded by a membrane
  9. 9. The membranes must be broken open in order to get the DNA out of the cell
  10. 10. Detergent: • Breaks apart membranes by attaching to the lipids (fats) & proteins in the membranes
  11. 11. The cell and nuclear membranes have been broken apart, as well as all of the organelle membranes, such as those around the mitochondria and chloroplasts. So what is left? • Proteins • Carbohydrates (sugars) • DNA
  12. 12. Add a pinch of meat tenderizer to the mixture
  13. 13. Why? • Meat tenderizer is an ENZYME – A protein that helps chemical reactions happen more quickly • The enzymes in meat tenderizer break apart proteins
  14. 14. The DNA in the nucleus is surrounded by proteins
  15. 15. The meat tenderizer cuts away the proteins from the DNA
  16. 16. Stir very, very GENTLY!
  17. 17. Tilt your cup & slowly pour a layer of alcohol on top of the pea soup mixture • DNA will float up into the alcohol from the pea soup

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