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INDIAN DENTAL ACADEMY
Leader in continuing dental education
www.ind...
Cytotoxicity of CommerciallyCytotoxicity of Commercially
Available Silicone MaterialAvailable Silicone Material
Used for M...
IntroductionIntroductionwww.indiandentalacademy.comwww.indiandentalacademy.com
Purpose of the StudyPurpose of the Study
 The purpose of this study was to
qualitatively assess the potential
cytotoxicit...
Materials & MethodMaterials & Method
A metallic die with diameter
-28mm and depth - 2mm was
fabricated to standardize the
...
Realistic groupBiomed group
Five samples for each group were fabricated.
www.indiandentalacademy.comwww.indiandentalacadem...
Pressure gauge indicating the
pressure applied
Autoclave unit
All the samples of both groups were sterilized in autoclave ...
Cells and MediumCells and Medium
Syrian hamster; kidney cells
in fetal bovine serum Eagle’s Minimum Essential
Medium (MEM)...
Selection of medium for serumSelection of medium for serum
 There are few good guidelines for selection of
appropriate me...
Cells and MediumCells and Medium
 Syrian hamster; kidney cells were used.
They were grown in Eagle’s Minimum
Essential Me...
Procedure to measure cellProcedure to measure cell
cytotoxicitycytotoxicity
 These samples were divided into three
groups...
 Control group – In this only the MEM
(Eagle’s medium) and hamster kidney
cells were taken
Petri dish showing the control...
Biomed group Realistic group.
 The silicon material samples were placed in
the culture media. Then the media was
removed ...
 The LAMINAR FLOWHOOD works on a function
of HEPA (High efficiency particulate air) to make the air
sterile.
 The microb...
 Incubation vials
with the samples
after transferring
the cell lines
www.indiandentalacademy.comwww.indiandentalacademy.c...
Incubator with the vials
 Samples were innoculated with cell lines and were
incubated for 3 days at 37°C and 5% carbon
di...
 The optimal temperature for the incubation is
dependent on three factors:-
1) Body temperature of the animal from which
...
 Cytotoxicity was observed by recording any change
in the morphologies of the syrian hamster kidney
cells (with the help ...
 OLYMPUS phase contrast microscope
www.indiandentalacademy.comwww.indiandentalacademy.com
ObservationsObservations
 Whirlpool pattern
arrangement of
syrian kidney cells
can be seen in the
control group under
the...
Micrographic view of Biomed group
after incubation shows morphological
damage of culture cells
www.indiandentalacademy.com...
Micrographic view of Realistic group
after incubation shows morphological
damage of culture cells
www.indiandentalacademy....
ResultsResults
GROUPGROUP CYTOTOXICITYCYTOTOXICITY
ControlControl 00
RealisticRealistic 11
BiomedBiomed 11
www.indiandenta...
Clinical ImplicationsClinical Implications
 The probability of allergic or toxic reactions
from silicon materials or thei...
DiscussionDiscussion
DiscussionDiscussionwww.indiandentalacademy.comwww.indiandentalacademy.com
LimitationsLimitations
 The sample size was less.
 It was the qualitative not the quantitative test.
 Only one method t...
For more details please visit
www.indiandentalacademy.com
www.indiandentalacademy.comwww.indiandentalacademy.com
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Cytotoxicity of silicone/ general dentistry services

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Transcript of "Cytotoxicity of silicone/ general dentistry services"

  1. 1. www.indiandentalacademy.comwww.indiandentalacademy.com INDIAN DENTAL ACADEMY Leader in continuing dental education www.indiandentalacademy.com
  2. 2. Cytotoxicity of CommerciallyCytotoxicity of Commercially Available Silicone MaterialAvailable Silicone Material Used for MaxillofacialUsed for Maxillofacial ProsthesisProsthesis www.indiandentalacademy.comwww.indiandentalacademy.com
  3. 3. IntroductionIntroductionwww.indiandentalacademy.comwww.indiandentalacademy.com
  4. 4. Purpose of the StudyPurpose of the Study  The purpose of this study was to qualitatively assess the potential cytotoxicity of various (Biomed and Realistic) commercially available silicon materials used vastly for maxillofacial prosthesis in prosthetic dentistry. www.indiandentalacademy.comwww.indiandentalacademy.com
  5. 5. Materials & MethodMaterials & Method A metallic die with diameter -28mm and depth - 2mm was fabricated to standardize the samples. Realistic and Biomed silicone materials. Two commercially available materials Biomed and Realistic were used to evaluate cytotoxicity. www.indiandentalacademy.comwww.indiandentalacademy.com
  6. 6. Realistic groupBiomed group Five samples for each group were fabricated. www.indiandentalacademy.comwww.indiandentalacademy.com
  7. 7. Pressure gauge indicating the pressure applied Autoclave unit All the samples of both groups were sterilized in autoclave at 121°C at 10 pounds of pressure for 30 minutes. The autoclave has an outer jet and an inner jet. First the pressure is created in the outer jet and then transferred in the inner jet. www.indiandentalacademy.comwww.indiandentalacademy.com
  8. 8. Cells and MediumCells and Medium Syrian hamster; kidney cells in fetal bovine serum Eagle’s Minimum Essential Medium (MEM) MEM is a complex media which contains a large number of amino acids and vitamins and is often supplemented with extra metabolites and minerals www.indiandentalacademy.comwww.indiandentalacademy.com
  9. 9. Selection of medium for serumSelection of medium for serum  There are few good guidelines for selection of appropriate medium for a given cell type, but it is based on:- 1) Literature or source of cells. 2) Otherwise choice is either empirical or by comparative testing of several media. www.indiandentalacademy.comwww.indiandentalacademy.com
  10. 10. Cells and MediumCells and Medium  Syrian hamster; kidney cells were used. They were grown in Eagle’s Minimum Essential Medium (MEM) with 2mM L-glutamine and Earle’s BSS adjusted to contain 1.5g/L Na bicarbonate, 0.1mM non-essential amino acids, and 1.0mM Na pyruvate, 90% fetal calf serum. www.indiandentalacademy.comwww.indiandentalacademy.com
  11. 11. Procedure to measure cellProcedure to measure cell cytotoxicitycytotoxicity  These samples were divided into three groups: 1. Control group. 2. Realistic group. 3. Biomed group. www.indiandentalacademy.comwww.indiandentalacademy.com
  12. 12.  Control group – In this only the MEM (Eagle’s medium) and hamster kidney cells were taken Petri dish showing the control group www.indiandentalacademy.comwww.indiandentalacademy.com
  13. 13. Biomed group Realistic group.  The silicon material samples were placed in the culture media. Then the media was removed by aspiration and placed in the vials. The cells were transferred to the vials. www.indiandentalacademy.comwww.indiandentalacademy.com
  14. 14.  The LAMINAR FLOWHOOD works on a function of HEPA (High efficiency particulate air) to make the air sterile.  The microbiologist is working under LAMINAR FLOWHOOD which is a sterilized chamber to maintain the non microbial environment.  Here the microbiologist is transferring the Syrian hamster; kidney cell line to the culture media. www.indiandentalacademy.comwww.indiandentalacademy.com
  15. 15.  Incubation vials with the samples after transferring the cell lines www.indiandentalacademy.comwww.indiandentalacademy.com
  16. 16. Incubator with the vials  Samples were innoculated with cell lines and were incubated for 3 days at 37°C and 5% carbon dioxide atmosphere. www.indiandentalacademy.comwww.indiandentalacademy.com
  17. 17.  The optimal temperature for the incubation is dependent on three factors:- 1) Body temperature of the animal from which the cells are obtained. 2) Any regional variation eg. cells from the skin. 3) Incorporation of safety factor to allow minor errors in incubator regulation. www.indiandentalacademy.comwww.indiandentalacademy.com
  18. 18.  Cytotoxicity was observed by recording any change in the morphologies of the syrian hamster kidney cells (with the help of the OLYMPUS phase contrast microscope) as compared with the controls and the results from these 3 groups were recorded and scored for each sample on a 0 - 4+T scale:  0  no cytotoxicity  1+T  1-25% effect  2+T  25%-50% effect  3+T  50%-75% effect on cells  4+T  75%-100% destruction of the cell www.indiandentalacademy.comwww.indiandentalacademy.com
  19. 19.  OLYMPUS phase contrast microscope www.indiandentalacademy.comwww.indiandentalacademy.com
  20. 20. ObservationsObservations  Whirlpool pattern arrangement of syrian kidney cells can be seen in the control group under the phase contrast microscope appearance Without filter With filterwww.indiandentalacademy.comwww.indiandentalacademy.com
  21. 21. Micrographic view of Biomed group after incubation shows morphological damage of culture cells www.indiandentalacademy.comwww.indiandentalacademy.com
  22. 22. Micrographic view of Realistic group after incubation shows morphological damage of culture cells www.indiandentalacademy.comwww.indiandentalacademy.com
  23. 23. ResultsResults GROUPGROUP CYTOTOXICITYCYTOTOXICITY ControlControl 00 RealisticRealistic 11 BiomedBiomed 11 www.indiandentalacademy.comwww.indiandentalacademy.com
  24. 24. Clinical ImplicationsClinical Implications  The probability of allergic or toxic reactions from silicon materials or their components is small but the following test results show that the potential exists.  The perceptive practitioner will be alert to the subtle signs of tissue response that may manifest itself with the use of silicon materials. www.indiandentalacademy.comwww.indiandentalacademy.com
  25. 25. DiscussionDiscussion DiscussionDiscussionwww.indiandentalacademy.comwww.indiandentalacademy.com
  26. 26. LimitationsLimitations  The sample size was less.  It was the qualitative not the quantitative test.  Only one method to evaluate the cytotoxicity was done.  Other methods to evaluate cytotoxicity such as cell viability, cell culture agarose overlay tests can be done to correlate the results. www.indiandentalacademy.comwww.indiandentalacademy.com
  27. 27. For more details please visit www.indiandentalacademy.com www.indiandentalacademy.comwww.indiandentalacademy.com

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