Diffusion Probes and pH Lab
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Diffusion Probes and pH Lab

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Students use Vernier probes to measure the change in pH as various concentrations of acids and bases diffuse through dialysis tubing.

Students use Vernier probes to measure the change in pH as various concentrations of acids and bases diffuse through dialysis tubing.

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    Diffusion Probes and pH Lab Diffusion Probes and pH Lab Presentation Transcript

    • Open your lab notebook to your table of contents and fill in the information below Date Title Partners Pages 7 October Diffusion & pH (will be assigned)
    • Turn to the next blank page in your lab notebook and write the following:
      • Title: Examining the rate of diffusion of unknown concentrations of acids and bases, into dH 2 O, using Vernier Probes.
    • Materials
      • Each group will have:
      • 1 probe
      • 1 beaker of dH2O
      • 1 sample to test
      • 1 clip to hold the sample
    • Write the following in your notebook.
      • Sample number: __________
      • Color of sample: ___________
      • Initial pH of dH 2 O: __________
      • Final pH of dH 2 O: __________
      Sample# Slope of the line (m) Molarity 1 2 3 4 5
    • IMPORTANT!
      • These substances can burn your skin
      • Do not get the Vernier probe bases wet
      • Be gentle with pH probes
    • Setting up your probe
      • Give the probe to one person in your group.
      • File, New
      • Click Mode
      • Click the white box next to “rate.” Type in 10
      • Click the white box next to “length.” Type in 5
      • Using the drop down menu and select “min.”
      • Click “OK.”
      • Plug in the pH probe into the top of the base where it says “CH 1.”
      • Place the base into the plastic bag
      • Unscrew the tube at the end of the pH probe
      • Place the probe into a beaker of dH 2 O
      • Watch the red box on the screen until the pH reaches between 7.3 and 7.5
      • Set up your sample
      • Press the play button on the screen of the base.
    • At the end of 5 minutes
      • Graph button
      • Analyze
      • Curve fit of pH
      • Choose fit, linear
      • Write down the “m” number
    • Sample# Slope of the line (m) Molarity 1 2M HCl 2 1M HCl 3 Water 4 1 M NaOH 5 2 M NaOH
    • What is Molarity?
      • Molarity is a measure of grams of a substance per liter.
      • M = g/L
      • The higher the Molarity, the higher the concentration of a substance.
    •  
    • Analysis
      • Draw the general shape of each graph
      • How does Molarity relate to rate of diffusion?
      • For each solution was the water hypotonic, hypertonic or isotonic to the sample in the bag?