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Study of Titanium Surface Modification & Collagen Grafting for dental implants
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Study of Titanium Surface Modification & Collagen Grafting for dental implants


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My research on Titanium surface modification using blasting, etching, 3-APTES and collagen grafting

My research on Titanium surface modification using blasting, etching, 3-APTES and collagen grafting

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  • Transcript

    • 1. Writer: 이흐완 히다얏 Advisor: Prof. 김석영 생체재료실험실
    • 2. Contents
      • Introduction
      • Research Background
      • Research Objectives
      • Research Methodology
      • Research Progress
      • Research Plans
    • 3. Introduction Nowadays Initially Research & Development Used to improve a patient’s masticator function Patients desired also the aesthetics , short treatment time and procedural reliability Improving Titanium’s surface activity , osseointegration and fixation Based on the discovery by Swedish Professor Per-Ingvar Brånemark , Titanium can be successfully fused into bone when osteoblasts grow on and into the rough surface of the implanted titanium Dental Implants Why Titanium?
    • 4. Research Background
      • The success of an implant depends on the ability of the material to attach with the living host tissue.
      • Improvement of fixation between implants and hard tissue can be achieved by coating the metal surface with Calcium Phosphates material or Aluminum Oxide.
      • Recent studies on a series of protein coatings on Ti have demonstrated their effective roles in the stimulation of cellular responses, such as attachment and proliferation .
    • 5. Research Background
      • Collagen , being the main constituting protein of the hard tissues, has the potential as a coating moiety on a Ti implant.
      • Hydroxyapatite (HA) coating has been particularly well investigated as a preliminary coating for collagen immobilization on titanium surfaces, because human bone is mainly composed of HA and collagen fibers.
      • However, the chemical bonding and the composition of HA-collagen is recognized as being difficult, because it involves two dissimilar organic and inorganic nanophases.
    • 6. Research Objectives
      • Experimental on Surface Modifications to improve Titanium surface activity
      • Immobilizing Collagen onto Titanium by using coupling agent
    • 7. R&D Flow Chart Substrate: Titanium Interface: Coupling agent Coating: Collagen (type 1) Dental implants Orthopedic implant OH radical Surface Processing Collagen coating Cell Attachment/ Proliferation Materials Processing in vitro Tests Applications
    • 8. Research Methodology Ti discs Polishing Treatment Heat Treatment TFAA treatment & XPS observation 3-APTES grafting SEM/EDS Observation Collagen Coating Cell Culture Blasting Chemical Treatment Surface Roughness Measurement Analysis
    • 9. Research Progress
      • Blasting treatment with HA and Al2O3 was done to compare the characteristics and to observe the cell growth.
      • Etching treatment with H3PO4 and HNO3 was also conducted to find the effect for both blasting materials.
      • Surface roughness:
        • The surface roughness average for HA blasted discs are lower than Al2O3 blasted discs
        • Etching reduced the roughness average as it removed the embedded blasted particles.
    • 10. Research Progress
      • The HA blasted Ti discs showed the presence of calcium and phosphorous on the surface of Titanium.
        • After HNO 3 etching the calcium and phosphorous were all removed from the surface.
        • After H 3 PO 4 etching , the calcium was removed from the surface meanwhile the phosphorous remained , it was found that titanium react with phosphorous , confirmed by XRD.
      • The Al 2 O 3 blasted Ti discs showed the presence of alumina on the surface and it remain on the surface after acid etching , both with HNO 3 and H 3 PO 4 . it needed stronger acid or longer etching time to remove Al 2 O 3 .
      Fig. 1 Al 2 O 3 Blasted #200 & H 3 PO 4 Etched Table 1. HA blasted Ti discs Table 2. HA blasted - HNO 3 etching Table 3. HA blasted - H 3 PO 4 etching Element Weight% Atomic% Compd% Formula O K 39.37 65.69 98.29 O2Ti P K 1.71 1.47 0.00 Ti 58.93 32.84 Totals 100.00 Element Weight% Atomic% Compd% Formula O K 40.05 66.67 100.00 O2Ti Ti 59.95 33.33 Totals 100.00 Element Weight% Atomic% O K 30.60 55.38 P K 4.88 4.56 Ca K 9.09 6.56 Ti K 55.43 33.50 Totals 100.00
    • 11. Research Progress
      • NaOH & H 2 O 2 etching were conducted to obtain OH - ( hydroxyl groups ) on Ti surface (see table).
      • The etched Ti surfaces were immersed with 3-APTES , as coupling agent between Titanium and Collagen, with 3 various time : 10 , 30 Minutes , and 1 Hour .
      • Surface Morphology of the treated discs were observed, after 3-APTES immersed, there were some difference on the NaOH etched, but not much changes on the H2O2
      (a) NaOH etched (b) NaOH + 3-APTES 10 Min (c) H 2 O 2 etched (d) H 2 O 2 + 3-APTES 10 Min Chemical Temperature Concentration Time (Hr) NaOH 70 o C 5 M 24, 12 H 2 O 2 80 o C 35.4 Vol % 48, 24
    • 12. Research Plans
      • Perform heat treatment (after etching treatment)
      • Observation on the Ti treated surface morphology
        • Confirmation of what compound are formed on the Ti surface & how many OH - groups formed on the Ti surface
        • Tri-fluoro acetic anhydride (TFAA) treatment
        • X-Ray Photoelectron Spectroscopy (XPS) observation
      • Collagen grafting
      • Bone cell test, to compare the cell attachment and cell growth on each treated Ti discs
    • 13. Terima Kasih Merci Xie xie Syukron Rahmat Danke Arigato Gracias Grazie Mahalo Salamat