IEA - O exemplo das abelhas sociais
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Palestra "O exemplo das abelhas sociais visto por uma análise causal do desenvolvimento"

Palestra "O exemplo das abelhas sociais visto por uma análise causal do desenvolvimento"

Palestra da Prof.ª Dra. Zilá luz Paulino Simões

27/04/2012

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    IEA - O exemplo das abelhas sociais IEA - O exemplo das abelhas sociais Presentation Transcript

    • Dieta e castas: o exemplo das abelhas sociais Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Laboratório de Biologia do Desenvolvimento de Abelhas http://zulu.fmrp.usp.br/beelab Pré- Genoma Pós-Genoma
    • Apis mellifera O modelo DietaUm mesmo genótipo
    • Interações gênicas e controle do crescimento Nijhout, FH, 2003
    • Uma diferença Os órgãos reprodutoresOperária Rainha
    • O terceiro par de pernas Rainha Operária
    • Os títulos de Hormônio Juvenil HJ Rainha Operária EcD
    • O genes são diferencialmente expressos - MicroarraysAngel R. Barchuk
    • Análise da região promotora dos genes superexpressos em rainhas e operárias de Apis mellifera – Reguladores? Alexandre dos Santos Cristino
    • Os títulos de Hormônio Juvenil HJ Rainha Operária EcDKlaus Hartfelder Usando esta informação........
    • Motivos localizados na região up stream dos genes diferencialmente expressosJH+ JH+
    • Sítio de ligação ao Ultraspiracle localizado na região up sptream dos genes diferencialmente expressos – tratamento com HJJH+ JH -
    • JH Altos Hsc70Cb Aumento de tamanhotítulos Tor Genes do fisiometabolismo Trap1 Queen Baixos usp Genes dotítulos Crc desenvolvimento RfaBp Alteração de caracteres morfológicos Worker
    • Eles fazem a diferençaPrincipalmente nas pernas posteriores, no terceiro par desenvolve a corbícula – ausente nas rainhas
    • No metathorax Rainha OperáriaAna Bomtorin
    • A região da corbícula Worker Queen
    • Expressão diferencial de Ubx no desenvolvimento do terceiro par de perna de operárias e rainhas de Apis mellifera. Azul, núcleos ;rosa, Ubx; vermelho, maior concentração de Ubx.
    • OperáriaEmbryo Dois fenótipos Dieta RainhaUm únicogenótipo
    • Diferenciação de castas em Apis mellifera Barchuk et al. 2007
    • Maleszka, R (2008)
    • Resposta a alterações na dietaModelo Apis mellifera testado em Drosophila melanogaster
    • Interações gênicas e controle do crescimento Nijhout, FH, 2003
    • Royalactin induces queen differentiation in honeybees Drosophila melanogaster Kamakura, 2011 Um trabalho que todos gostariam de ter feito .......
    • Efeito da supressão de proteínas da dieta de Drosophila melanogaster Drosophila melanogaster Li et al, 2010
    • Níveis do Hormônio Juvenil são controlados por uma esterase Aline MackertExpressão do gene codificador da esterase do HJ ao longo do desenvolvimento de operárias e rainhas de Apis mellifera Aline Aleixo
    • Quais os miRNAs afetados pelo silenciamento da JHE? 3 grupos Controle (10)Sequenciamento dos pequenos RNAs 3 grupos Tratados (10) 14 miRNAs diferencialmente expressos ame-miR-3728 ame-miR-317 ame-miR-3727 ame-miR-3719 ame-miR-3759 ame-miR-996 ame-miR-3793 ame-miR-100 ame-miR-3720 ame-miR-13b ame-miR-3477 ame-miR-306 ame-miR-316 ame-miR-263b
    • Silenciamento do gene codificador da esterase do Hormônio Juvenil
    • N. Reads > 101200000010000000 8000000 JC01 6000000 JC03 4000000 JC06 2000000 0 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 36 N. Reads > 1050000004500000400000035000003000000 JT012500000 JT022000000 JT0415000001000000 500000 0 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 36 Flávia Freitas
    • Quais os genes candidatos a alvo desses miRNAs? Busca pelos genes-alvo dos miRNAs -RNAhybrid -14 miRNAs X 11062 genes preditos Resultou em 1011 interações, das quais 562 envolvem genes-alvo com ortólogos em Drosophila melanogaster.Quais os processos biológicos regulados por esses 14 miRNAs?
    • Data: 16/04/2012 OrtólogosTarefa: miRNAs diferencialmente expressos (tagwise) x Genoma Total = 1011 interações Total = 562 interações energia =< - 20 kcal/mol ; pvalue < 0.05; seed 2-7 energia =< - 20 kcal/mol ; pvalue < 0.05; seed 2-7miRNAs selecionados miRNA target mfe pvalue miRNA target ortholog mfe pvalueame-miR-3728 ame-miR-3728 GB17247 -30,4 0,001483 ame-miR-3728 GB17247 CG4272 -30,4 0,001483ame-miR-3727 ame-miR-3728 GB13779 -25,9 0,024596 ame-miR-3728 GB13779 PIP5K59B -25,9 0,024596ame-miR-3759 ame-miR-3728 GB14496 -25,5 0,031491 ame-miR-3728 GB14496 CG11034 -25,5 0,031491ame-miR-3793 ame-miR-3728 GB10640 -26,4 0,01804 ame-miR-3728 GB10640 Tollo -26,4 0,01804ame-miR-3720 ame-miR-3728 GB12348 -24,9 0,045534 ame-miR-3728 GB12348 CG31952 -24,9 0,045534ame-miR-3477 ame-miR-3728 GB14721 -25 0,042828 ame-miR-3728 GB14326 CG5018 -26,9 0,013219ame-miR-316 ame-miR-3728 GB30423 -27,9 0,007086 ame-miR-3728 GB11944 G-salpha60A -25,4 0,033493ame-miR-317 ame-miR-3728 GB30095 -27,3 0,010304 ame-miR-3728 GB17292 CG13467 -24,9 0,045534ame-miR-3719 ame-miR-3728 GB14326 -26,9 0,013219 ame-miR-3728 GB15316 CG8603 -25,1 0,04028ame-miR-996 ame-miR-3728 GB18338 -25,5 0,031491 ame-miR-3728 GB14809 endoA -25,8 0,026165ame-miR-100 ame-miR-3728 GB30112 -25,1 0,04028 ame-miR-3728 GB16081 CG32666 -26,3 0,019195ame-miR-13b ame-miR-3728 GB11944 -25,4 0,033493 ame-miR-3728 GB11728 CG2972 -25,1 0,04028ame-miR-306 ame-miR-3728 GB17292 -24,9 0,045534 ame-miR-3728 GB19710 CG4660 -27,4 0,009681ame-miR-263b ame-miR-3728 GB15316 -25,1 0,04028 ame-miR-3728 GB11325 su(w[a]) -25,6 0,029607 ame-miR-3728 GB15237 -26,8 0,014068 ame-miR-3728 GB19918 CG5036 -25 0,043891Genes preditos (versão 4.0 do genoma - OGS2) ame-miR-3728 GB17395 -26,8 0,014068 ame-miR-3728 GB17750 CG8320 -26,2 0,02042411062 genes ame-miR-3728 GB14809 -25,8 0,026165 ame-miR-3728 GB17322 CG7031 -25,7 0,027662 ame-miR-3728 GB12191 -24,8 0,048406 ame-miR-3728 GB19438 CG4342 -25,4 0,033493 ame-miR-3728 GB16081 -26,3 0,019195 ame-miR-3728 GB15084 CG5928 -29,1 0,003326 ame-miR-3728 GB11728 -25,1 0,04028 ame-miR-3728 GB18069 CG3437 -23,3 0,011338 ame-miR-3728 GB19710 -27,4 0,009681 ame-miR-3728 GB18723 CG32052 -25,3 0,03562 ame-miR-3728 GB11306 -27,8 0,007543 ame-miR-3728 GB19944 CG10869 -24,8 0,048406 ame-miR-3728 GB15906 -26,7 0,014971 ame-miR-3728 GB10091 CG7802 -25,1 0,04028 ame-miR-3728 GB11325 -25,6 0,029607 ame-miR-3728 GB10096 slmb -26,6 0,015932 ame-miR-3728 GB19918 -25 0,043891 ame-miR-3728 GB16957 mfr -25,1 0,04028 ame-miR-3728 GB17750 -26,2 0,020424 ame-miR-3728 GB14902 CG4945 -26,7 0,014971 ame-miR-3728 GB11580 -24,8 0,048406 ame-miR-3727 GB11321 CG30437 -27,3 0,03829 ame-miR-3728 GB17322 -25,7 0,027662 ame-miR-3727 GB12933 CG33041 -27,6 0,032413 ame-miR-3728 GB19438 -25,4 0,033493 ame-miR-3727 GB13779 PIP5K59B -32,4 0,002181 ame-miR-3728 GB15084 -29,1 0,003326 ame-miR-3727 GB10640 Tollo -27,2 0,040472 ame-miR-3728 GB17301 -29,9 0,002029 ame-miR-3727 GB14534 CG17758 -29,1 0,014009 ame-miR-3728 GB13233 -26,5 0,011174 ame-miR-3727 GB19937 SoxN -28,3 0,021935 ame-miR-3728 GB14111 -25,8 0,026165 ame-miR-3727 GB10999 CG11132 -30,9 0,005085 ame-miR-3728 GB12697 -25,9 0,024596 ame-miR-3727 GB15897 CG13855 -28,7 0,017534 ame-miR-3728 GB18069 -23,3 0,011338 ame-miR-3727 GB19853 Bgb -27,2 0,040472 ame-miR-3728 GB17136 -26,2 0,020424 ame-miR-3727 GB17100 svp -31,2 0,004293 ame-miR-3728 GB16378 -22,1 0,017946 ame-miR-3727 GB18573 CG8005 -28,8 0,016578 ame-miR-3728 GB18723 -25,3 0,03562 ame-miR-3727 GB19191 Scgdelta -28,7 0,017145
    • Comparação com os resultados de 2007
    • JH Altos Hsc70Cb Aumento de tamanhotítulos Tor Genes do fisiometabolismo Trap1 Queen Baixos usp Genes dotítulos Crc desenvolvimento RfaBp Alteração de caracteres morfológicos Worker
    • GO ID Description p-val corr p-val cluster_freq cluster_freq% total_freq total_freq% 9987 cellular process 7,54E-06 1,03E-04 249/364 68,40% 5484/9550 57,40% 32501 multicellular organismal process 5,01E-15 7,12E-13 175/364 48,00% 2777/9550 29,00% 50794 regulation of cellular process 1,24E-22 7,62E-20 153/364 42,00% 1913/9550 20,00% 32502 developmental process 1,57E-14 1,71E-12 148/364 40,60% 2200/9550 23,00% 7275 multicellular organismal development 2,39E-15 3,68E-13 138/364 37,90% 1941/9550 20,30% 48856 anatomical structure development 1,37E-14 1,68E-12 133/364 36,50% 1876/9550 19,60% 16043 cellular component organization 8,40E-09 2,35E-07 116/364 31,80% 1866/9550 19,50% 48731 system development 1,54E-14 1,71E-12 112/364 30,70% 1452/9550 15,20% 9653 anatomical structure morphogenesis 2,07E-16 9,56E-14 108/364 29,60% 1293/9550 13,50% 51179 localization 1,75E-07 3,64E-06 105/364 28,80% 1723/9550 18,00% 19222 regulation of metabolic process 4,49E-16 1,11E-13 100/364 27,40% 1158/9550 12,10% 48513 organ development 1,46E-13 1,17E-11 94/364 25,80% 1150/9550 12,00% 31323 regulation of cellular metabolic process 2,13E-15 3,58E-13 92/364 25,20% 1039/9550 10,80% 60255 regulation of macromolecule metabolic process 6,60E-16 1,36E-13 92/364 25,20% 1020/9550 10,60% 80090 regulation of primary metabolic process 4,82E-16 1,11E-13 92/364 25,20% 1015/9550 10,60% 48869 cellular developmental process 2,50E-13 1,85E-11 91/364 25,00% 1104/9550 11,50% 30154 cell differentiation 1,34E-12 7,99E-11 87/364 23,90% 1061/9550 11,10% 10468 regulation of gene expression 1,23E-15 2,27E-13 85/364 23,30% 907/9550 9,40% 51171 regulation of nitrogen compound metabolic process 4,82E-16 1,11E-13 83/364 22,80% 859/9550 8,90% 19219 regulation of nucleobase nucleoside nucleotide and nucleic acid metabolic process 4,20E-16 1,11E-13 83/364 22,80% 857/9550 8,90% 23052 signaling 2,72E-08 6,80E-07 78/364 21,40% 1102/9550 11,50% 9889 regulation of biosynthetic process 8,05E-13 5,13E-11 75/364 20,60% 835/9550 8,70% 31326 regulation of cellular biosynthetic process 8,05E-13 5,13E-11 75/364 20,60% 835/9550 8,70% 51234 establishment of localization 3,23E-03 1,67E-02 75/364 20,60% 1457/9550 15,20% 48468 cell development 8,19E-11 3,16E-09 72/364 19,70% 862/9550 9,00% 10556 regulation of macromolecule biosynthetic process 2,34E-12 1,35E-10 72/364 19,70% 800/9550 8,30% 6810 transport 4,66E-03 2,19E-02 72/364 19,70% 1408/9550 14,70% 51252 regulation of RNA metabolic process 1,15E-14 1,51E-12 68/364 18,60% 655/9550 6,80% 50896 response to stimulus 4,79E-04 3,71E-03 68/364 18,60% 1205/9550 12,60% 7399 nervous system development 6,99E-12 3,32E-10 67/364 18,40% 731/9550 7,60% 45449 regulation of transcription 6,17E-12 3,07E-10 67/364 18,40% 729/9550 7,60% 9887 organ morphogenesis 6,34E-13 4,34E-11 65/364 17,80% 661/9550 6,90% Predição de genes alvos dos micros diferencialmente expressos pelo silenciamento da Esterase do Hormônio Juvenil
    • OperáriaEmbryo Dois fenótipos Dieta RainhaUm únicogenótipo
    • Nutrition Reproduction miRNAs Body growth insulina/TOR/IGF Ovary development Royal pathway Physiometabolic genes Queen JH Jelly Royalactin Brain JHE Neurogenesis Corpora Worker specific organs allata Developmental genes Larval Food Body Growth miRNAs insulina/TOR/IGF Ovary Development pathway Physiometabolic genes Worker JH Worker Food Royalactin Cérebro Neurogenesis JHE Corpora Worker specific organs allata Developmental genes Modificado de Malezska, 2008
    • 14 miRNAs Diferencialmente expressos1945 Processos Biológicos Alta porcentagem de genes do desenvolvimento
    • ÀFAPESPCNPqCAPES
    • GO ID Description p-val corr p-val cluster_freq cluster_freq% total_freq total_freq% 9987 cellular process 7,54E-06 1,03E-04 249/364 68,40% 5484/9550 57,40% 32501 multicellular organismal process 5,01E-15 7,12E-13 175/364 48,00% 2777/9550 29,00% 50794 regulation of cellular process 1,24E-22 7,62E-20 153/364 42,00% 1913/9550 20,00% 32502 developmental process 1,57E-14 1,71E-12 148/364 40,60% 2200/9550 23,00% 7275 multicellular organismal development 2,39E-15 3,68E-13 138/364 37,90% 1941/9550 20,30% 48856 anatomical structure development 1,37E-14 1,68E-12 133/364 36,50% 1876/9550 19,60% 16043 cellular component organization 8,40E-09 2,35E-07 116/364 31,80% 1866/9550 19,50% 48731 system development 1,54E-14 1,71E-12 112/364 30,70% 1452/9550 15,20% 9653 anatomical structure morphogenesis 2,07E-16 9,56E-14 108/364 29,60% 1293/9550 13,50% 51179 localization 1,75E-07 3,64E-06 105/364 28,80% 1723/9550 18,00% 19222 regulation of metabolic process 4,49E-16 1,11E-13 100/364 27,40% 1158/9550 12,10% 48513 organ development 1,46E-13 1,17E-11 94/364 25,80% 1150/9550 12,00% 31323 regulation of cellular metabolic process 2,13E-15 3,58E-13 92/364 25,20% 1039/9550 10,80% 60255 regulation of macromolecule metabolic process 6,60E-16 1,36E-13 92/364 25,20% 1020/9550 10,60% 80090 regulation of primary metabolic process 4,82E-16 1,11E-13 92/364 25,20% 1015/9550 10,60% 48869 cellular developmental process 2,50E-13 1,85E-11 91/364 25,00% 1104/9550 11,50% 30154 cell differentiation 1,34E-12 7,99E-11 87/364 23,90% 1061/9550 11,10% 10468 regulation of gene expression 1,23E-15 2,27E-13 85/364 23,30% 907/9550 9,40% 51171 regulation of nitrogen compound metabolic process 4,82E-16 1,11E-13 83/364 22,80% 859/9550 8,90% 19219 regulation of nucleobase nucleoside nucleotide and nucleic acid metabolic process 4,20E-16 1,11E-13 83/364 22,80% 857/9550 8,90% 23052 signaling 2,72E-08 6,80E-07 78/364 21,40% 1102/9550 11,50% 9889 regulation of biosynthetic process 8,05E-13 5,13E-11 75/364 20,60% 835/9550 8,70% 31326 regulation of cellular biosynthetic process 8,05E-13 5,13E-11 75/364 20,60% 835/9550 8,70% 51234 establishment of localization 3,23E-03 1,67E-02 75/364 20,60% 1457/9550 15,20% 48468 cell development 8,19E-11 3,16E-09 72/364 19,70% 862/9550 9,00% 10556 regulation of macromolecule biosynthetic process 2,34E-12 1,35E-10 72/364 19,70% 800/9550 8,30% 6810 transport 4,66E-03 2,19E-02 72/364 19,70% 1408/9550 14,70% 51252 regulation of RNA metabolic process 1,15E-14 1,51E-12 68/364 18,60% 655/9550 6,80% 50896 response to stimulus 4,79E-04 3,71E-03 68/364 18,60% 1205/9550 12,60% 7399 nervous system development 6,99E-12 3,32E-10 67/364 18,40% 731/9550 7,60% 45449 regulation of transcription 6,17E-12 3,07E-10 67/364 18,40% 729/9550 7,60% 9887 organ morphogenesis 6,34E-13 4,34E-11 65/364 17,80% 661/9550 6,90% Predição de genes alvos dos micros diferencialmente expressos pelo silenciamento da Esterase do Hormônio Juvenil