Your SlideShare is downloading. ×
0
Exercises 5: Post-Laboratory Discussion for  Sterilization
v Set-up B: Set-up A: Set-up C: Set-up D: Non-sterile medium in a Sterile Petri dish Sterile medium in a Sterile Petri dis...
Results
After 24 hours of incubation
Non-sterile medium in a Sterile Petri dish
Sterile medium in a Sterile Petri dish
Non-sterile medium in a Non-sterile Petri dish
Sterile medium in a Non-sterile Petri dish
After 48 hours of incubation
Non-sterile medium in a Sterile Petri dish
Sterile medium in a Sterile Petri dish
Non-sterile medium in a Non-sterile Petri dish
Sterile medium in a Non-sterile Petri dish
Discussion
For Non-sterile Media: The microorganisms form mostly at the center of the medium. For Non-sterile Petri dishes: At first,...
Death Rate α [Microorganisms] Thermal Death Time (TDT) -time required to kill a known population of microorganisms in a sp...
Significance of this experiment: In order to have a successful experiment, the medium and the Petri dish must be properly ...
Answers to Guide Questions
<ul><li>How does moist heat and dry heat sterilization eliminate microbial growth? </li></ul>Dry Heat Moist Heat Kills by ...
2. Explain the difference when performing sterilization in a microbiology laboratory in UP Baguio as compared to UP Manila...
3. Account for the differences in the microbial growth (absent or present) in the four set-ups. Set-up Surface Inner Colon...
~ K a m s a h a m n i d a ~ ^ - ^
Upcoming SlideShare
Loading in...5
×

Exercise 5-post lab of sterilization

1,020

Published on

a Post-lab discussion for an exercise in BIO 120 Laboratory.

Published in: Education, Technology, Business
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
1,020
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
11
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "Exercise 5-post lab of sterilization"

  1. 1. Exercises 5: Post-Laboratory Discussion for Sterilization
  2. 2. v Set-up B: Set-up A: Set-up C: Set-up D: Non-sterile medium in a Sterile Petri dish Sterile medium in a Sterile Petri dish Non-sterile medium in a Non-sterile Petri dish Sterile medium in a Non-sterile Petri dish
  3. 3. Results
  4. 4. After 24 hours of incubation
  5. 5. Non-sterile medium in a Sterile Petri dish
  6. 6. Sterile medium in a Sterile Petri dish
  7. 7. Non-sterile medium in a Non-sterile Petri dish
  8. 8. Sterile medium in a Non-sterile Petri dish
  9. 9. After 48 hours of incubation
  10. 10. Non-sterile medium in a Sterile Petri dish
  11. 11. Sterile medium in a Sterile Petri dish
  12. 12. Non-sterile medium in a Non-sterile Petri dish
  13. 13. Sterile medium in a Non-sterile Petri dish
  14. 14. Discussion
  15. 15. For Non-sterile Media: The microorganisms form mostly at the center of the medium. For Non-sterile Petri dishes: At first, the microorganisms form mostly in between the wall and the dish. Then, they spread at the surface of the medium.
  16. 16. Death Rate α [Microorganisms] Thermal Death Time (TDT) -time required to kill a known population of microorganisms in a specific suspension at a particular temperature. ↑ Temperature, ↓ TDT and ↓ Temperature, ↑ TDT
  17. 17. Significance of this experiment: In order to have a successful experiment, the medium and the Petri dish must be properly sterilized.
  18. 18. Answers to Guide Questions
  19. 19. <ul><li>How does moist heat and dry heat sterilization eliminate microbial growth? </li></ul>Dry Heat Moist Heat Kills by protein coagulation/ denaturation of enzymes and essential proteins. There is a breakage of H-bonds that holds protein 3-D structure Kills by destructive oxidation of the essential cell constituents rather than protein coagulation
  20. 20. 2. Explain the difference when performing sterilization in a microbiology laboratory in UP Baguio as compared to UP Manila. What necessary adjustments may be done? There is no difference. Why? We are just concern with the system inside a pressure cooker or an autoclave which is not affected by the pressure and temperature of the outside environment. We can still manage to sterilize culture media and materials in an autoclave (121 o C, 15 psi, 15 mins.) without making any adjustments.
  21. 21. 3. Account for the differences in the microbial growth (absent or present) in the four set-ups. Set-up Surface Inner Colony (groups) nsM-sP Present Present Present sM-sP Absent Absent Absent nsM-nsP Present Present Absent sM-nsP Present Absent Present
  22. 22. ~ K a m s a h a m n i d a ~ ^ - ^
  1. A particular slide catching your eye?

    Clipping is a handy way to collect important slides you want to go back to later.

×