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Biobrick An adjective, not a noun http://en.wikipedia.org/wiki/Biobrick
Overview <ul><li>Short introduction to biobrick parts and plasmids </li></ul><ul><li>How to make plasmids with biobrick pa...
Biobrick part in a plasmid http://partsregistry.org/Help:An_Introduction_to_BioBricks
The plasmid (backbone) http://partsregistry.org/Help:Plasmids http://partsregistry.org/Help:Plasmid_backbones/Glossary E E...
Construction plasmids http://partsregistry.org/Part:BBa_P1010
Prefix & Suffix Prefix: GAATTC GCGGCCGC T TCTAGA G CTTCCG CGCCGGCG A AGATCT C Suffix: T ACTAGT A GCGGCCG CTGCAG A TGATCA T...
Standard assembly http://partsregistry.org/Help:Standard_Assembly_%28zoom%29
RBS-CDS issues http://partsregistry.org/Assembly:RBS-CDS_issues XbaI + SpeI (RBS)T A^CTAG  A G(CDS) (RBS)A T  GATC^T C(CDS...
Parallel/Rolling assembly http://partsregistry.org/Assembly:Rolling_assembly
Robotic assembly <ul><li>epMotion5075 </li></ul>http://partsregistry.org/Assembly:Robotic_assembly
Questions <ul><li>http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919 </li></ul>
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Biobricks

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Transcript of "Biobricks"

  1. 1. Biobrick An adjective, not a noun http://en.wikipedia.org/wiki/Biobrick
  2. 2. Overview <ul><li>Short introduction to biobrick parts and plasmids </li></ul><ul><li>How to make plasmids with biobrick parts </li></ul><ul><li>How to combine the biobrick parts in one plasmid </li></ul>
  3. 3. Biobrick part in a plasmid http://partsregistry.org/Help:An_Introduction_to_BioBricks
  4. 4. The plasmid (backbone) http://partsregistry.org/Help:Plasmids http://partsregistry.org/Help:Plasmid_backbones/Glossary E EcoRI X XbaI S SpeI P PstI
  5. 5. Construction plasmids http://partsregistry.org/Part:BBa_P1010
  6. 6. Prefix & Suffix Prefix: GAATTC GCGGCCGC T TCTAGA G CTTCCG CGCCGGCG A AGATCT C Suffix: T ACTAGT A GCGGCCG CTGCAG A TGATCA T CGCCGGC GACGTC EcoRI XbaI SpeI PstI NotI Prefix cut with XbaI: ...GCT T^CTAG A G ...CGA A GATC^T C Suffix cut with SpeI: T A^CTAG T AGC... A T GATC^A TCG... SpeI + XbaI ...T A^CTAG A G... ...A T GATC^T C... http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix http://partsregistry.org/Help:Plasmid_backbones/Glossary
  7. 7. Standard assembly http://partsregistry.org/Help:Standard_Assembly_%28zoom%29
  8. 8. RBS-CDS issues http://partsregistry.org/Assembly:RBS-CDS_issues XbaI + SpeI (RBS)T A^CTAG A G(CDS) (RBS)A T GATC^T C(CDS) Scars: Normal:(RBS) T ACTAG A G (CDS) Scar of 8 bases With T:(RBS) T A CTAG ATG (CDS-ATG) Scar of 6 bases
  9. 9. Parallel/Rolling assembly http://partsregistry.org/Assembly:Rolling_assembly
  10. 10. Robotic assembly <ul><li>epMotion5075 </li></ul>http://partsregistry.org/Assembly:Robotic_assembly
  11. 11. Questions <ul><li>http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919 </li></ul>
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