Biobricks
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Biobricks

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    Biobricks Biobricks Presentation Transcript

    • Biobrick An adjective, not a noun http://en.wikipedia.org/wiki/Biobrick
    • Overview
      • Short introduction to biobrick parts and plasmids
      • How to make plasmids with biobrick parts
      • How to combine the biobrick parts in one plasmid
    • Biobrick part in a plasmid http://partsregistry.org/Help:An_Introduction_to_BioBricks
    • The plasmid (backbone) http://partsregistry.org/Help:Plasmids http://partsregistry.org/Help:Plasmid_backbones/Glossary E EcoRI X XbaI S SpeI P PstI
    • Construction plasmids http://partsregistry.org/Part:BBa_P1010
    • Prefix & Suffix Prefix: GAATTC GCGGCCGC T TCTAGA G CTTCCG CGCCGGCG A AGATCT C Suffix: T ACTAGT A GCGGCCG CTGCAG A TGATCA T CGCCGGC GACGTC EcoRI XbaI SpeI PstI NotI Prefix cut with XbaI: ...GCT T^CTAG A G ...CGA A GATC^T C Suffix cut with SpeI: T A^CTAG T AGC... A T GATC^A TCG... SpeI + XbaI ...T A^CTAG A G... ...A T GATC^T C... http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix http://partsregistry.org/Help:Plasmid_backbones/Glossary
    • Standard assembly http://partsregistry.org/Help:Standard_Assembly_%28zoom%29
    • RBS-CDS issues http://partsregistry.org/Assembly:RBS-CDS_issues XbaI + SpeI (RBS)T A^CTAG A G(CDS) (RBS)A T GATC^T C(CDS) Scars: Normal:(RBS) T ACTAG A G (CDS) Scar of 8 bases With T:(RBS) T A CTAG ATG (CDS-ATG) Scar of 6 bases
    • Parallel/Rolling assembly http://partsregistry.org/Assembly:Rolling_assembly
    • Robotic assembly
      • epMotion5075
      http://partsregistry.org/Assembly:Robotic_assembly
    • Questions
      • http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919