A. nidulans iGEM Wageningen UR
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  • 1. A short introduction
  • 2.
    • Phylogenetic tree
    • A. nidulans specific
    • A. nidulans as a model organism
    • Reproduction cycles
    • A. nidulans vs. A. niger
  • 3.
    • Largest Phylum of fungi (over 64.000) species
    • Defining sexual feature: the ascus.
  • 4.
    • Filamentous fungi
    • Fission rather then budding
    Binary Fission Budding
  • 5.
      • Cleistothecium
    plate of medium with A nidulans culture Order: Eurotiales The green and blue molds
  • 6.
    • Aggresive colonization strategies
    • Adaptable to extreme environmental conditions
      • For A. nidulans :
        • Temperature 10-45 o C
        • pH 2.5-11
        • NaCl concentration <4.0 M
  • 7.
    • Highly aerobic
    • Oligotrophy
    • Commercially the most important fungi
    Citric acid http://aspergillusblog.blogspot.com/2008/09/aspergillus-produced-most-of-worlds.html http://en.wikipedia.org/wiki/Aspergillus
  • 8.
    • Creates a cleistothecium with ascus inside in the sexual cycle
    • Filamentous
    • Cells divide by fission
    • Green cultures
    • Can live under ‘extreme’ conditions
    • Highly aerobic
    • oligotroph
  • 9.
    • Synonym: Aspergillus nidulellus
    • Teleomorph: Emericella nidulans
    • Anamorph: Aspergillus nidulans
    • Homothallic
    • Sexual, asexual and parasexual reproduction
    • Model organism
  • 10. http://www.nature.com/nrg/journal/v3/n9/box/nrg889_BX1.html
  • 11.
    • Why A. nidulans has been chosen as a model organism
    http://www.broadinstitute.org/annotation/genome/aspergillus_group/MultiHome.html
  • 12. http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_F1.html
  • 13. http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_F1.html
  • 14. http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_F1.html
  • 15.
    • Pulmonary infection
    • Cutaneous aspergillosis
    • Maxillary sinus disease
    • Guttural pouch mycosis (in horses)
    • Cerebral abscess
    • Endophtalmitis
    • Osteomyelitis
    • Superficial infections
    • Disseminated infections
    The atlas of clinical fungi p486 http://www.doctorfungus.org/thefungi/Aspergillus_nidulans.php
  • 16.
    • Cell biology
    • Gene regulation
    • Mitosis
    • Intracellular functions of mitotic motors
    • Carbon & nitrogen regulation
    • Cell cycle
    • Fungal protein secretion
    • Gene regulation
    • Cell wall biosynthesis
    • Cell morphology
    • Carbon metabolism
    • Nutrient transport
    • Effects of environmental factors on enzyme production
    • Industrial use
    http://www.broadinstitute.org/annotation/genome/aspergillus_group/GenomeDescriptions.html
  • 17.
    • Sexual cycle
    • 8 chromosomes
    • At least 9 cell types
    • Research focused on gene control
    • No sexual cycle
    • 8 chromosomes
    • Research focused on protein production and excretion
    www.broadinstitute.org/annotation/fungi/fgi/sample_whitepaper.pdf nature.com/nbt/journal/v25/n2/fig_tab/nbt1282_F2.html www.broadinstitute.org/annotation/genome/aspergillus_group/GenomeDescriptions.html