Biological control of Pierce’s disease: Influence of grape genotype, plant growth stage, and geographic location D. L. Hop...
Biological Control of PD with strain EB92-1 <ul><li>Grape Genotypes </li></ul><ul><li>Mature, Producing Grapevine </li></u...
Current tests on new grape plantings of various grape genotypes Field injections
Greenhouse injection
<ul><li>Effect of strain EB92-1 on PD in various genotypes,  V. vinifera  (Cabernet Franc),  V. aestivalis  (Cynthiana), a...
Effect of strain EB92-1 on PD in various cultivars in Central Florida
Additional Genotype Studies <ul><li>North Carolina –  Mature plants of  Vitis labrusca cultivars  Canadice, Concord Seedle...
Inoculated approximately 10 7  cells EB92-1 per plant into 2 nd  and 3 rd  internodes. Mature Plant Treatments
Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a high incidence of PD
Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in North Georgia vineyard 29 36 10 6
Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a low incidence of PD
Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in a North Georgia vineyard 3 4 (2)
Planned Mature Plant Study for 2009 Inject EB92-1 into the main trunk of the vines to get more systemic affect
North Georgia Central Florida North Carolina South Carolina Different Locations (Climates)
Effect of EB92-1 on development of PD in new plantings in Florida (4 years - Cabernet Franc, Chardonnay, Chenin Blanc, Gol...
Comparison of treatment method with EB92-1 on control of Pierce’s disease in  rooted cuttings in the greenhouse. b b a b a...
Effect of EB92-1 on host colonization by the virulent Pierce’s disease strain <ul><li>Rooted cuttings of Carignane grapevi...
10 5  PD92-9 cells Colonization of host plant by EB92-1  and pathogen (PD92-9   10 5  PD92-9 cells
Summary <ul><li>Grape Genotypes  –  In Florida, EB92-1 was effective in  Vitis vinifera  cultivars,  V. aestivalis,  and A...
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Influence of grape genotype, plant growth stage, and geographic location on Pierce's Disease - Don Hopkins - Pierce's Disease Conference 2008

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Influence of grape genotype, plant growth stage, and geographic location on Pierce's Disease - Don Hopkins - Pierce's Disease Conference 2008

  1. 1. Biological control of Pierce’s disease: Influence of grape genotype, plant growth stage, and geographic location D. L. Hopkins Mid-Florida REC University of Florida Phil Brannen Plant Pathology Dept. University of Georgia
  2. 2. Biological Control of PD with strain EB92-1 <ul><li>Grape Genotypes </li></ul><ul><li>Mature, Producing Grapevine </li></ul><ul><li>Different Geographic Areas </li></ul><ul><li>Treatment Methodology </li></ul><ul><li>Colonization </li></ul>
  3. 3. Current tests on new grape plantings of various grape genotypes Field injections
  4. 4. Greenhouse injection
  5. 5. <ul><li>Effect of strain EB92-1 on PD in various genotypes, V. vinifera (Cabernet Franc), V. aestivalis (Cynthiana), and French/American hybrid (Chambourcin) in the vineyard in Central Florida. </li></ul>
  6. 6. Effect of strain EB92-1 on PD in various cultivars in Central Florida
  7. 7. Additional Genotype Studies <ul><li>North Carolina – Mature plants of Vitis labrusca cultivars Canadice, Concord Seedless, Einset, Glenora, Himrod, Interlaken, Jupiter, Lakemont, Marquis, Mars, Reliance, Suffolk Red, Vanessa, and Venus. </li></ul><ul><li>South Carolina – Young transplants of Vitis labrusca cultivars Jupiter, Neptune, Mars, and Venus . </li></ul><ul><li>Georgia – Young transplants of Cabernet Sauvignon, Cabernet Franc, and Vidal Blanc (hybrid). </li></ul><ul><li>California – Young transplants of Cabernet Sauvignon, Orange Muscat, Barbera, Viognier, Reisling, and Chardonnay. </li></ul>
  8. 8. Inoculated approximately 10 7 cells EB92-1 per plant into 2 nd and 3 rd internodes. Mature Plant Treatments
  9. 9. Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a high incidence of PD
  10. 10. Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in North Georgia vineyard 29 36 10 6
  11. 11. Effect of EB92-1 on development of PD in mature ‘Mourvedre’ grapevines in North Georgia vineyard with a low incidence of PD
  12. 12. Effect of EB92-1 on survival of symptomatic, mature ‘Mourvedre’ grapevines in a North Georgia vineyard 3 4 (2)
  13. 13. Planned Mature Plant Study for 2009 Inject EB92-1 into the main trunk of the vines to get more systemic affect
  14. 14. North Georgia Central Florida North Carolina South Carolina Different Locations (Climates)
  15. 15. Effect of EB92-1 on development of PD in new plantings in Florida (4 years - Cabernet Franc, Chardonnay, Chenin Blanc, Golden Muscat, Ruby Cabernet), Georgia (1 year – Cabernet Sauvignon, Cab. Franc, Vidal Blanc), S. Carolina (1 year – Neptune)
  16. 16. Comparison of treatment method with EB92-1 on control of Pierce’s disease in rooted cuttings in the greenhouse. b b a b a a EB92-1 Treatment Methodology
  17. 17. Effect of EB92-1 on host colonization by the virulent Pierce’s disease strain <ul><li>Rooted cuttings of Carignane grapevine with >18 inches of new growth were used. </li></ul><ul><li>Plants were inoculated with a suspension of EB92-1 (10 8 cells/ml). One drop was inoculated into the 1 st and 2 nd internodes (total 10 7 cells/plant). </li></ul><ul><li>Three weeks later, plants were inoculated with a suspension of PD92-9, a virulent PD strain (10 6 cells/ml). One drop was inoculated into opposite sides of the 3 rd internode (10 5 cells/plant). </li></ul><ul><li>The 3 rd internode (0, pathogen-inoculated internode) and the 13 th internode were sampled for grinding and dilution plating. </li></ul><ul><li>EB92-1 and PD92-9 were labeled with different colored fluorescent proteins for differentiation on culture plates. </li></ul>
  18. 18. 10 5 PD92-9 cells Colonization of host plant by EB92-1 and pathogen (PD92-9 10 5 PD92-9 cells
  19. 19. Summary <ul><li>Grape Genotypes – In Florida, EB92-1 was effective in Vitis vinifera cultivars, V. aestivalis, and American hybrids. Tests are in progress on other cultivars in other locations. </li></ul><ul><li>Mature, Producing Grapevine - Treatment of mature grapevines did not reduce new cases of PD in the first year, but may have had a slight benefit in year 2 and 3. Treated vines that developed PD symptoms had a better survival rate in year 2 and 3 than the untreated. </li></ul><ul><li>Different Geographic Areas – Biological control has been very effective on transplants in Florida. Preliminary data is positive in Georgia and South Carolina. </li></ul><ul><li>Treatment Methodology - Propagating rooted cuttings from mother vines infected with EB92-1 was inconsistent in biological control of Pierce’s disease, providing control in Chambourcin and not in Chardonnay. </li></ul><ul><li>Colonization - In greenhouse inoculations, EB92-1 prevented the pathogenic Pierce’s disease strain from reaching a threshold population in the plant needed to produce disease. </li></ul>
  20. 20. Thanks!

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