Pratyush Harsh and Sourav Nayak 3
• It grows anaerobically if nitrate is available.
• Growth occurs at wide range of
tempe...
Petri dish
Beaker
Conical Flask
Incubator
Autoclave
Laminar air flow
Beef extract
Peptone
Agar Powder
Inoculation loop
Step 1:-
• Take 100ml of water in a beaker.
• Add -Agar powder (1.3g per 100ml),
Peptone (4g per 100ml),
Beef Extract (4g ...
Step 2:-
• Maintain the ph of the mixture to be
7.5 with NaOH.
• Cover the flask with a piece of cotton
plug and aluminium...
Step 3:-
• Keep the medium and petriplates in the
autoclave with some water at constant
Pressure of 15 lbs and temperature...
Step 4:-
• Take the conical flask and pour out the
liquified medium in a sterile petri plate
immediately to avoid solidifi...
Step 5:-
• Cover the medium plate with another
petri dish. Care should be taken that
excess medium should not be poured in...
Step 6:-
• If the Petri Dish is completely cooled
then place it in the refrigerator for
about 24 hours.
Step 7:-
• Take it out from refrigerator and allow it
to come to room temperature. Take the
bacteria from subculture and m...
Step 8:-
• On streaking the bacteria are added to
the culture. Now allow it to multiply in
the dish ,keep this plate in th...
• We see a growth of the bacteria on the
plate after 2 to 3 days. Now carefully
collect the bacteria in a test tube add
wa...
• Prepare the soil sample ready along
with the plastics and add the bacteria
mixture directly to the soil or in a
polythen...
• Keep another soil sample with same
amount of plastics but without the
bacterium added to it. Observe the
mixtures after ...
• This shows that the bacteria has the
ability to decompose plastics .This is
only a small example to show that the
proces...
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Culture of bacteria
Upcoming SlideShare
Loading in …5
×

Culture of bacteria

291 views

Published on

it is about how can we culture pseudomonas bacteria

Published in: Education, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
291
On SlideShare
0
From Embeds
0
Number of Embeds
2
Actions
Shares
0
Downloads
28
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Culture of bacteria

  1. 1. Pratyush Harsh and Sourav Nayak 3 • It grows anaerobically if nitrate is available. • Growth occurs at wide range of temperatures 6-420C the optimum being 370C. • Growth on ordinary media producing large opaque irregular colonies with distinctive musty mawkish or earthy smell.
  2. 2. Petri dish
  3. 3. Beaker
  4. 4. Conical Flask
  5. 5. Incubator
  6. 6. Autoclave
  7. 7. Laminar air flow
  8. 8. Beef extract
  9. 9. Peptone
  10. 10. Agar Powder
  11. 11. Inoculation loop
  12. 12. Step 1:- • Take 100ml of water in a beaker. • Add -Agar powder (1.3g per 100ml), Peptone (4g per 100ml), Beef Extract (4g per 100ml)
  13. 13. Step 2:- • Maintain the ph of the mixture to be 7.5 with NaOH. • Cover the flask with a piece of cotton plug and aluminium foil.
  14. 14. Step 3:- • Keep the medium and petriplates in the autoclave with some water at constant Pressure of 15 lbs and temperature 121 0C for 20 minutes.
  15. 15. Step 4:- • Take the conical flask and pour out the liquified medium in a sterile petri plate immediately to avoid solidification of medium.
  16. 16. Step 5:- • Cover the medium plate with another petri dish. Care should be taken that excess medium should not be poured in the plate which may cause the contamination.
  17. 17. Step 6:- • If the Petri Dish is completely cooled then place it in the refrigerator for about 24 hours.
  18. 18. Step 7:- • Take it out from refrigerator and allow it to come to room temperature. Take the bacteria from subculture and make marks as shown in the picture with Inoculation loop. Take care that the inoculation loop is sterilized. The streaks can be done 3 to 4 times regularly as shown in figure. The complete process should be done in a laminar air flow.
  19. 19. Step 8:- • On streaking the bacteria are added to the culture. Now allow it to multiply in the dish ,keep this plate in the incubator to prevent the infections from other species.
  20. 20. • We see a growth of the bacteria on the plate after 2 to 3 days. Now carefully collect the bacteria in a test tube add water to it .Now we have a pure bacterial solution.
  21. 21. • Prepare the soil sample ready along with the plastics and add the bacteria mixture directly to the soil or in a polythene cover. Add some amount of sodium acetate that would enhance the process of degradation of plastics.
  22. 22. • Keep another soil sample with same amount of plastics but without the bacterium added to it. Observe the mixtures after every week and record the observations .The weight of the plastics in both the soil samples are collected. Observe it for about 3-4 weeks. We observe that the plastics in the soil sample containing the bacteria are degraded more than the other.
  23. 23. • This shows that the bacteria has the ability to decompose plastics .This is only a small example to show that the process happens ,but on a large scale the effect would be much beneficial to the environment.

×