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  • 1. Controlling Microbial Growth WebquestName:___________<br />Use Google and the various sites listed to help answer the questions below.<br />
    • Define an antiseptic
    • 2. What was the first type of antispeptic used in hospitals (chemical group)? What types of chemicals are more commonly used today?
    • 3. What is chlorhexidine? Draw the structure and give an example of where it is commonly found.
    • 4. Define a disinfectant.
    • 5. What is hypochlorate? List three common uses of hypochlorate. At which pH and temperature are they most efficient and why?
    • 6. Define bacteriocidal and bacteriostatic
    • 7. Visit the following website:
    • 8. http://www.yteach.co.uk/page.php/resources/view_all?id=aseptic_conditions_osmosis_gram_positive_bacterium_spontaneous_generation_pasteurisation_autoclave_fungi_spores_bacteria_manometer_thermometer_refrigerator_freezer_antibiotic_page_1&full=1&w_size=1024&h_size=768
    • 9. Watch the slideshow and then try the activities.
    • 10. Summarise the process of pasteurization below.
    • 11. Have a look at this website
    • 12. http://www.gcsescience.com/pwav53.htm
    • 13. What is gamma radiation used for? Why do you think its use is controversial?
    • 14. Visit the following website
    • 15. http://sakshat.amrita.ac.in/VirtualLab/index.php?sub=BIOTECH&brch=MIC&sim=Streak_Plate_Method&cnt=theory
    • 16. Use the website to define the following key terms and ideas:
    • 17. Culture media:
    • 18. Agar:
    • 19. Culture:
    • 20. Streak plate:
    • 21. Lawn:
    • 22. Watch the animation on streaking and then try it yourself here! http://learn.chm.msu.edu/vibl/content/streakplate.html
    • 23. “Viruses are obligate parasites” Explain how culturing viruses in a lab is different to culturing bacteria in a lab.
    • 24. Counting cells
    • 25. Using a haemocytometer
    • 26. http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Resources.html+
    • 27. Read the sections on ‘Introduction to a hameocytometer’ and ‘Counting cells’
    Draw a picture of a haemocytometer <br />
    • Have a go on this virtual lab on turbidometry http://water.me.vccs.edu/courses/env211/lab4_print.htm
    • 28. Describe how microbiologists use turbidometry to assesss microbial contamination of waater.