SlideShare a Scribd company logo
1 of 23
Download to read offline
ISS Programme 2011
         on
 School Science Lab
 Safety Regulations
Aseptic Techniques


A procedure performed under sterile condition
It is to prevent microbial contamination
It also protects the person who works on it from
infection
Aseptic Techniques in Microbial Work

 Always sterilise the work bench with 70% ethanol
 Prepare agar plates in laminar flow hood
 Always wear gloves
 All instruments for microbial work must be heat
 sterilised or autoclaved before using
 Work near a flame, as it creates a convection current
 which prevents contaminants from settling on the
 sample
Safety Precautions in
         Microbial Work
Heat sterilise the inoculating loop with a flame until red
hot after using.
Dip disposable inoculating loop in 10% chlorox before
disposal.
Dispose all biohazard waste in the biohazard bag.
Autoclave all biohazard waste before disposal.
Autoclaving

It make use of a device known as autoclave to sterilise
apparatus or waste
It sterilises apparatus or waste using high pressure at
121oC for 15 - 20 minutes
Autoclave tape is normally pasted on the item to
indicate whether a specific temperature has been
reached
Autoclave Tape

It’s a tape similar to masking tape but it is more
adhesive and can withstand the hot and moist
conditions of the autoclave
The tape has diagonal markings that contains an ink
which changes colour from beige to black upon
heating
Biological Safety Cabinet
 There are 3 classes of biological safety cabinet
 Class I - Protect the person against contamination
 from the sample.
 Class II - Designed for use with low to moderate risk
 biological agents and provide product, personnel, and
 environmental protection.
 Class III - Provide a physical barrier between biological
 agents and personnel.
Air flow in Class I biological
        safety cabinet
Air flow in Class II biological
        safety cabinet
Air flow in Class II biological
        safety cabinet
Laminar Flow Cabinet
This is often mistaken for a biosafety cabinet as they
look the same.
Laminar Flow cabinet only protect samples inside the
working area from external airborne contamination.
It does not protect user against contamination from the
sample.
Thus biohazardous activities (like bacteria work) should
never be carried out in it.
Air Flow in Laminar Flow
        Cabinet
Use of micro-centrifuge
 Tubes in the centrifuge must be balanced.
 Dummy tubes or tubes filled with an appropriate
 amount of water should be used if there is an odd
 number of tubes needed to be centrifuge.
 Micro-centrifuge should never be moved while in
 operation
 Never open a micro-centrifuge lid
 while the rotor is moving.
Good Laboratory Practice

Petri dishes containing microbial cultures must be
labelled with the following before incubating :
    Name of the micro-organism / sample
    Date of start of culture
    Date of completion of culture
    Name of student and teacher in charge
Dealing with minor micro-
    organism spillage
Always report a spillage to the teacher or technician
before cleaning up yourself.
For minor spillage, cover the spill area with a piece of
tissue and poured disinfectant (like Dettol or bleach
solution) over it and left it to stand for at least 30
minutes.
Wear gloves and dispose the stained tissue into
biohazard bag for autoclaving.
Dealing with major micro-
    organism spillage

Report the spillage to the teacher or lab technician.
Teacher or lab technician will assess the severity of the
spillage and if necessary all personnel in the room
should leave the laboratory immediately.
Cleaning of the spillage will be done by lab technician
with proper protective clothing.
Dealing with specific accidents
 involving micro-organisms
Injection, cuts & abrasions - Remove clothing of the
affected part. Gently squeeze the wound to encourage
slight bleeding. Wash the affected part and apply
appropriate antiseptic.
Ingestion of micro-organisms - Rinse the mouth
and drink several glasses of water and induce vomiting.
This can be done by stimulating the back of the throat
with the tip of a finger.
Dealing with specific accidents
 involving micro-organisms
Micro-organism in contact with skin - Remove any
contaminated clothing. Washed affected area with soap and
water. Apply antiseptic if micro-organism is in contact with
broken skin.
Micro-organism in contact with eyes - Flush the eyes with
running water using the eye washers for a minimum of 15
minutes.
  Always consult a doctor immediately and inform the
 doctor of the nature of the wound and micro-organism
    after applying the first aid as mentioned above.
Chemicals
Chemical can be classified into the following table with
its corresponding effect.
Classification                    Effects
                  Flammable properties even when not in contact
   Oxidising            with other combustible material

  Flammable                     Low Flash Point

   Corrosive                  Can cause skin burns

     Irritant        Can cause significant skin inflammation

  Mutagenic            Can cause heritable genetic damage

 Carcinogenic                  Can cause cancer
Dealing with chemical
            spillage

Report to teacher or lab technician when spillage
occurs.
Wear gloves if the chemical is hazardous.
Use tissue to soak up the spillage followed by wiping
the area with a damp tissue.
Mercury spillage will be handled by the lab technician,
do not clear up mercury spillage by yourself.
Dealing with accidents
     involving chemicals
  First aid administrated is the same as dealing
  with accident in contact with micro-organism.
Chemical burns - Remove any clothing or shoes that
is contacted with the chemical. Wash the injured area
with cool running water for at least 10 minutes.
If possible, soak the injured area in cold water to numb
the wound. Do not apply any burn ointment to affected
area. Dry the wound and cover with a sterile gauze.
Electrical Injury

Switch off the supply affecting the casualty and remove
the person from contact with the apparatus.
Inform the teacher or lab technician to call ambulance
by dialling 995 if they access it as a serious situation.
If there is a trained personnel, do artificial resuscitation
and cardiac massage immediately.
Cuts and bleeding
Minor case - Remove dirt or glass, wash under
running water and apply clean, dry, sterile dressing (like
plaster)
Serious case - Let the casualty lie down. Remove dirt
or glass by washing under running water. Apply
pressure to the wound with a thick pad of gauze and a
firm bandage. Call 995 for ambulance.
  In any case of emergency, please inform the
teacher or lab technician before applying the first
                aid to the casualty.

More Related Content

What's hot

Aseptic Technique
Aseptic TechniqueAseptic Technique
Aseptic Techniquedeathful
 
Surgical hand scrub_protocol_updates
Surgical hand scrub_protocol_updatesSurgical hand scrub_protocol_updates
Surgical hand scrub_protocol_updatesipdsiot
 
Sterile Technique Powerpoint
Sterile Technique PowerpointSterile Technique Powerpoint
Sterile Technique Powerpointjennisnell
 
INFECTION CONTROL Ahana A.
INFECTION CONTROL   Ahana A.INFECTION CONTROL   Ahana A.
INFECTION CONTROL Ahana A.Ahana Ajayakumar
 
Hh pest control_100410
Hh pest control_100410Hh pest control_100410
Hh pest control_100410Khoirul Anam
 
Care of linen
Care of linen Care of linen
Care of linen Rajani17
 
Monis, clemcy pearl a. (asepsis and infection control)
Monis, clemcy pearl a. (asepsis and infection control)Monis, clemcy pearl a. (asepsis and infection control)
Monis, clemcy pearl a. (asepsis and infection control)ClemcyPearlMonis
 
Accidents in lab and remedies
Accidents in lab and remediesAccidents in lab and remedies
Accidents in lab and remediesfathimakk
 
universal precautions
 universal precautions universal precautions
universal precautionsSabari Nathan
 
10 don't miss practical summary for your safety
10 don't miss  practical summary for your safety10 don't miss  practical summary for your safety
10 don't miss practical summary for your safetyaakaricls
 
Dental Lab safety
Dental Lab safetyDental Lab safety
Dental Lab safetyDentechUMP
 
Prevention Of Hospital Infection
Prevention Of Hospital InfectionPrevention Of Hospital Infection
Prevention Of Hospital Infectionshabeel pn
 
Safety and specimen preparation black
Safety and specimen preparation   blackSafety and specimen preparation   black
Safety and specimen preparation blackrihannagib
 
Lab safety and PPE and Biosafety levels
Lab safety and PPE and Biosafety levelsLab safety and PPE and Biosafety levels
Lab safety and PPE and Biosafety levelsharini challapalli
 
Surgical Instrument Sterilization
Surgical Instrument SterilizationSurgical Instrument Sterilization
Surgical Instrument SterilizationSurgical Solutions
 

What's hot (20)

Lab safety
Lab safetyLab safety
Lab safety
 
Aseptic Technique
Aseptic TechniqueAseptic Technique
Aseptic Technique
 
AsepticTechnique
AsepticTechnique AsepticTechnique
AsepticTechnique
 
Surgical hand scrub_protocol_updates
Surgical hand scrub_protocol_updatesSurgical hand scrub_protocol_updates
Surgical hand scrub_protocol_updates
 
Sterile Technique Powerpoint
Sterile Technique PowerpointSterile Technique Powerpoint
Sterile Technique Powerpoint
 
INFECTION CONTROL Ahana A.
INFECTION CONTROL   Ahana A.INFECTION CONTROL   Ahana A.
INFECTION CONTROL Ahana A.
 
Hh pest control_100410
Hh pest control_100410Hh pest control_100410
Hh pest control_100410
 
Aseptic Technique
Aseptic TechniqueAseptic Technique
Aseptic Technique
 
Care of linen
Care of linen Care of linen
Care of linen
 
Monis, clemcy pearl a. (asepsis and infection control)
Monis, clemcy pearl a. (asepsis and infection control)Monis, clemcy pearl a. (asepsis and infection control)
Monis, clemcy pearl a. (asepsis and infection control)
 
Accidents in lab and remedies
Accidents in lab and remediesAccidents in lab and remedies
Accidents in lab and remedies
 
universal precautions
 universal precautions universal precautions
universal precautions
 
10 don't miss practical summary for your safety
10 don't miss  practical summary for your safety10 don't miss  practical summary for your safety
10 don't miss practical summary for your safety
 
Dental Lab safety
Dental Lab safetyDental Lab safety
Dental Lab safety
 
Prevention Of Hospital Infection
Prevention Of Hospital InfectionPrevention Of Hospital Infection
Prevention Of Hospital Infection
 
Safety and specimen preparation black
Safety and specimen preparation   blackSafety and specimen preparation   black
Safety and specimen preparation black
 
Lab safety and PPE and Biosafety levels
Lab safety and PPE and Biosafety levelsLab safety and PPE and Biosafety levels
Lab safety and PPE and Biosafety levels
 
Surgical Instrument Sterilization
Surgical Instrument SterilizationSurgical Instrument Sterilization
Surgical Instrument Sterilization
 
Micrylium Disinfection
Micrylium DisinfectionMicrylium Disinfection
Micrylium Disinfection
 
Princilples of aseptic
Princilples of aseptic Princilples of aseptic
Princilples of aseptic
 

Similar to Iss programme 2011

lecture 10.Procedures for Handling Hazardous Spills
lecture 10.Procedures for Handling Hazardous Spillslecture 10.Procedures for Handling Hazardous Spills
lecture 10.Procedures for Handling Hazardous SpillsRaghda alomari
 
Asepsis, sterilization and infection control
Asepsis, sterilization and infection controlAsepsis, sterilization and infection control
Asepsis, sterilization and infection controlDr. Meenal Atharkar
 
Module 6 emergency response
Module 6 emergency responseModule 6 emergency response
Module 6 emergency responseEhealthMoHS
 
Safety rules in a histopathology laboratory.pptx
Safety rules in a histopathology laboratory.pptxSafety rules in a histopathology laboratory.pptx
Safety rules in a histopathology laboratory.pptxHawa Muweesi
 
Laboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationLaboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationNadjo Deen
 
Safety and specimen preparation black
Safety and specimen preparation   blackSafety and specimen preparation   black
Safety and specimen preparation blackrihannagib
 
safety and biosecurity (lab 10).Procedures for Handling Hazardous Spills
safety and biosecurity (lab 10).Procedures for Handling Hazardous Spillssafety and biosecurity (lab 10).Procedures for Handling Hazardous Spills
safety and biosecurity (lab 10).Procedures for Handling Hazardous SpillsRaghda alomari
 
SAFETY PRECAUTION in pathology lab while handling samples
SAFETY PRECAUTION in pathology lab while handling samplesSAFETY PRECAUTION in pathology lab while handling samples
SAFETY PRECAUTION in pathology lab while handling samplesNiveditaDevi1
 
Cross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxCross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxamiramna351
 
control hazards and risks.pptx
control hazards and risks.pptxcontrol hazards and risks.pptx
control hazards and risks.pptxnerissadollente1
 
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...Dr. Manuel Concepción
 
Mira 316 c sds
Mira 316 c sdsMira 316 c sds
Mira 316 c sdsiMovR
 
[Gen. surg] asepsis and antisepsis from SIMS Lahore
[Gen. surg] asepsis and antisepsis from SIMS Lahore[Gen. surg] asepsis and antisepsis from SIMS Lahore
[Gen. surg] asepsis and antisepsis from SIMS LahoreMuhammad Ahmad
 
Aseptic techniques in plant tissue culture
Aseptic techniques in  plant tissue cultureAseptic techniques in  plant tissue culture
Aseptic techniques in plant tissue culturekumarkanika
 

Similar to Iss programme 2011 (20)

lecture 10.Procedures for Handling Hazardous Spills
lecture 10.Procedures for Handling Hazardous Spillslecture 10.Procedures for Handling Hazardous Spills
lecture 10.Procedures for Handling Hazardous Spills
 
UCM SOP
UCM SOPUCM SOP
UCM SOP
 
Asepsis, sterilization and infection control
Asepsis, sterilization and infection controlAsepsis, sterilization and infection control
Asepsis, sterilization and infection control
 
Module 6 emergency response
Module 6 emergency responseModule 6 emergency response
Module 6 emergency response
 
Safety rules in a histopathology laboratory.pptx
Safety rules in a histopathology laboratory.pptxSafety rules in a histopathology laboratory.pptx
Safety rules in a histopathology laboratory.pptx
 
Laboratory hazards, safety and contamination
Laboratory hazards, safety and contaminationLaboratory hazards, safety and contamination
Laboratory hazards, safety and contamination
 
Safety and specimen preparation black
Safety and specimen preparation   blackSafety and specimen preparation   black
Safety and specimen preparation black
 
safety and biosecurity (lab 10).Procedures for Handling Hazardous Spills
safety and biosecurity (lab 10).Procedures for Handling Hazardous Spillssafety and biosecurity (lab 10).Procedures for Handling Hazardous Spills
safety and biosecurity (lab 10).Procedures for Handling Hazardous Spills
 
SAFETY PRECAUTION in pathology lab while handling samples
SAFETY PRECAUTION in pathology lab while handling samplesSAFETY PRECAUTION in pathology lab while handling samples
SAFETY PRECAUTION in pathology lab while handling samples
 
Cross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxCross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptx
 
control hazards and risks.pptx
control hazards and risks.pptxcontrol hazards and risks.pptx
control hazards and risks.pptx
 
CHEMICAL.PPT
CHEMICAL.PPTCHEMICAL.PPT
CHEMICAL.PPT
 
1573549i.ppt
1573549i.ppt1573549i.ppt
1573549i.ppt
 
Cleaning Decontamination, Disinfection and Sterilization Process
Cleaning Decontamination, Disinfection and Sterilization ProcessCleaning Decontamination, Disinfection and Sterilization Process
Cleaning Decontamination, Disinfection and Sterilization Process
 
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...
ATCC y otros reguladores internacionales de bioderivados. Introduction to mic...
 
Mira 316 c sds
Mira 316 c sdsMira 316 c sds
Mira 316 c sds
 
Dental Ic
Dental IcDental Ic
Dental Ic
 
Chapter 4 Ahs 104
Chapter 4 Ahs 104Chapter 4 Ahs 104
Chapter 4 Ahs 104
 
[Gen. surg] asepsis and antisepsis from SIMS Lahore
[Gen. surg] asepsis and antisepsis from SIMS Lahore[Gen. surg] asepsis and antisepsis from SIMS Lahore
[Gen. surg] asepsis and antisepsis from SIMS Lahore
 
Aseptic techniques in plant tissue culture
Aseptic techniques in  plant tissue cultureAseptic techniques in  plant tissue culture
Aseptic techniques in plant tissue culture
 

Iss programme 2011

  • 1. ISS Programme 2011 on School Science Lab Safety Regulations
  • 2. Aseptic Techniques A procedure performed under sterile condition It is to prevent microbial contamination It also protects the person who works on it from infection
  • 3. Aseptic Techniques in Microbial Work Always sterilise the work bench with 70% ethanol Prepare agar plates in laminar flow hood Always wear gloves All instruments for microbial work must be heat sterilised or autoclaved before using Work near a flame, as it creates a convection current which prevents contaminants from settling on the sample
  • 4. Safety Precautions in Microbial Work Heat sterilise the inoculating loop with a flame until red hot after using. Dip disposable inoculating loop in 10% chlorox before disposal. Dispose all biohazard waste in the biohazard bag. Autoclave all biohazard waste before disposal.
  • 5. Autoclaving It make use of a device known as autoclave to sterilise apparatus or waste It sterilises apparatus or waste using high pressure at 121oC for 15 - 20 minutes Autoclave tape is normally pasted on the item to indicate whether a specific temperature has been reached
  • 6. Autoclave Tape It’s a tape similar to masking tape but it is more adhesive and can withstand the hot and moist conditions of the autoclave The tape has diagonal markings that contains an ink which changes colour from beige to black upon heating
  • 7. Biological Safety Cabinet There are 3 classes of biological safety cabinet Class I - Protect the person against contamination from the sample. Class II - Designed for use with low to moderate risk biological agents and provide product, personnel, and environmental protection. Class III - Provide a physical barrier between biological agents and personnel.
  • 8. Air flow in Class I biological safety cabinet
  • 9. Air flow in Class II biological safety cabinet
  • 10. Air flow in Class II biological safety cabinet
  • 11. Laminar Flow Cabinet This is often mistaken for a biosafety cabinet as they look the same. Laminar Flow cabinet only protect samples inside the working area from external airborne contamination. It does not protect user against contamination from the sample. Thus biohazardous activities (like bacteria work) should never be carried out in it.
  • 12. Air Flow in Laminar Flow Cabinet
  • 13. Use of micro-centrifuge Tubes in the centrifuge must be balanced. Dummy tubes or tubes filled with an appropriate amount of water should be used if there is an odd number of tubes needed to be centrifuge. Micro-centrifuge should never be moved while in operation Never open a micro-centrifuge lid while the rotor is moving.
  • 14. Good Laboratory Practice Petri dishes containing microbial cultures must be labelled with the following before incubating : Name of the micro-organism / sample Date of start of culture Date of completion of culture Name of student and teacher in charge
  • 15. Dealing with minor micro- organism spillage Always report a spillage to the teacher or technician before cleaning up yourself. For minor spillage, cover the spill area with a piece of tissue and poured disinfectant (like Dettol or bleach solution) over it and left it to stand for at least 30 minutes. Wear gloves and dispose the stained tissue into biohazard bag for autoclaving.
  • 16. Dealing with major micro- organism spillage Report the spillage to the teacher or lab technician. Teacher or lab technician will assess the severity of the spillage and if necessary all personnel in the room should leave the laboratory immediately. Cleaning of the spillage will be done by lab technician with proper protective clothing.
  • 17. Dealing with specific accidents involving micro-organisms Injection, cuts & abrasions - Remove clothing of the affected part. Gently squeeze the wound to encourage slight bleeding. Wash the affected part and apply appropriate antiseptic. Ingestion of micro-organisms - Rinse the mouth and drink several glasses of water and induce vomiting. This can be done by stimulating the back of the throat with the tip of a finger.
  • 18. Dealing with specific accidents involving micro-organisms Micro-organism in contact with skin - Remove any contaminated clothing. Washed affected area with soap and water. Apply antiseptic if micro-organism is in contact with broken skin. Micro-organism in contact with eyes - Flush the eyes with running water using the eye washers for a minimum of 15 minutes. Always consult a doctor immediately and inform the doctor of the nature of the wound and micro-organism after applying the first aid as mentioned above.
  • 19. Chemicals Chemical can be classified into the following table with its corresponding effect. Classification Effects Flammable properties even when not in contact Oxidising with other combustible material Flammable Low Flash Point Corrosive Can cause skin burns Irritant Can cause significant skin inflammation Mutagenic Can cause heritable genetic damage Carcinogenic Can cause cancer
  • 20. Dealing with chemical spillage Report to teacher or lab technician when spillage occurs. Wear gloves if the chemical is hazardous. Use tissue to soak up the spillage followed by wiping the area with a damp tissue. Mercury spillage will be handled by the lab technician, do not clear up mercury spillage by yourself.
  • 21. Dealing with accidents involving chemicals First aid administrated is the same as dealing with accident in contact with micro-organism. Chemical burns - Remove any clothing or shoes that is contacted with the chemical. Wash the injured area with cool running water for at least 10 minutes. If possible, soak the injured area in cold water to numb the wound. Do not apply any burn ointment to affected area. Dry the wound and cover with a sterile gauze.
  • 22. Electrical Injury Switch off the supply affecting the casualty and remove the person from contact with the apparatus. Inform the teacher or lab technician to call ambulance by dialling 995 if they access it as a serious situation. If there is a trained personnel, do artificial resuscitation and cardiac massage immediately.
  • 23. Cuts and bleeding Minor case - Remove dirt or glass, wash under running water and apply clean, dry, sterile dressing (like plaster) Serious case - Let the casualty lie down. Remove dirt or glass by washing under running water. Apply pressure to the wound with a thick pad of gauze and a firm bandage. Call 995 for ambulance. In any case of emergency, please inform the teacher or lab technician before applying the first aid to the casualty.

Editor's Notes

  1. \n
  2. \n
  3. \n
  4. \n
  5. \n
  6. \n
  7. \n
  8. \n
  9. \n
  10. \n
  11. \n
  12. \n
  13. \n
  14. \n
  15. \n
  16. \n
  17. \n
  18. \n
  19. \n
  20. \n
  21. \n
  22. \n
  23. \n
  24. \n