plasmid isolation.HARIS

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plasmid isolation.HARIS

  1. 1. HARIS P MSC BIOTECHNOLOGY M.A.COLLEGE KOTHAMANGALAM ISOLATION OF PLASMID DNA BY ALKALINE LYSIS WITH SDS
  2. 2. <ul><li>Plasmid is an extra chromosomal DNA molecule which is capable of self replication. </li></ul><ul><li>It is circular and double stranded. </li></ul><ul><li>Plasmids naturally occur in bacteria but are also found in eukaryotic yeast. </li></ul>
  3. 3. HOW PLASMIDS ARE ISOLATED??? <ul><li>3 MAIN STEPS:- </li></ul><ul><li>CELL LYSIS </li></ul><ul><li>DEPROTEINATION & REMOVAL OF Chr.DNA </li></ul><ul><li>PRECIPITATION OF PLASMID DNA </li></ul>
  4. 4. FOR WHAT ????
  5. 5. THANK YOU....

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