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Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
Phase contrst microscope.HRS
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Phase contrst microscope.HRS

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Transcript

  • 1.  
  • 2. PHASE CONTRAST MICROSCOPY
  • 3.
    • Phase contrast microscope is a technique which makes highly transparent objects more visible without killing them.
    • Invented by the Dutch physicist Frederick
    • Zernike in 1930
  • 4. PRINCIPLE
    • It converts the different degrees of retardation of light rays passing through a translucent material into amplitude differences to create an image with increased contrast.
  • 5. COMPONENTS
    • Light source
    • Annular diaphragm
    • Condenser lens
    • Specimen stage
    • Objective lens
    • Phase plate
    • Telescopic lens
    • Eye piece
  • 6.  
  • 7.  
  • 8.  
  • 9.  
  • 10. Cheek cell
  • 11. Clostridium botulinum, the bacterium responsible for botulism, with subterminal oval endospores;
  • 12. Spirillum volutans, a very large bacterium with flagellar bundles
  • 13. Paramecium stained to show a large central macronucleus with a small spherical micronucleus at its side
  • 14. APPLICATIONS
    • Objects with poor intensity can be resolved well.
    • Image of the unstained object can be obtained
    • It is widely used in microbiology and tissue culture research to detect bacteria, cellular organelles and other small entities in living specimens
    • It allows the visualization of living unstained cells and their organelles.
    • For the detection of bacterial components such as endospores and inclusion bodies containing poly β hydroxy butyrate, polymetaphosphate, sulpher and other substances
  • 15. THANK YOU HARIS P MSC BIOTECHNOLOGY MA COLLEGE ERNAKULAM

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