New%20 Microsoft%20 Power Point%20 Presentation[1]

1,039 views

Published on

Mastitis in cows.

Published in: Education, Technology, Business
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
1,039
On SlideShare
0
From Embeds
0
Number of Embeds
8
Actions
Shares
0
Downloads
18
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

New%20 Microsoft%20 Power Point%20 Presentation[1]

  1. 1. A Project Report On " Presence of Mastitis & Isolation of different microbes from cow milk sample” Submitted By HARISH SHARMA Msc. (Microbiology) MIET MEERUT
  2. 2. <ul><li>ABSTRACT </li></ul><ul><li>Mastitis is one of the most costly diseases of dairy animals. </li></ul><ul><li>Out of five three samples showed the presence of E.coli. </li></ul><ul><li>Three showed the presence of Enterobacter aerogens. </li></ul><ul><li>two showed the presence of Staphylococcus aureus. Aspergillus niger was also present in three of the samples. </li></ul>
  3. 3. <ul><li>INTRODUCTION </li></ul><ul><li>Mastitis is an inflammation of the mammary gland or udder of the eve caused by bacteria that enter the udder through the teat end. </li></ul><ul><li>Mastitis can be either Clinical or Sub-clinical . </li></ul><ul><li>Mastitis causative factors. </li></ul><ul><li>Source and Pathophysiology. </li></ul>
  4. 4. <ul><li>OBJECTIVE </li></ul><ul><li>To collect the samples from various stations. </li></ul><ul><li>Measurement of pH through pH meter. </li></ul><ul><li>Conformational test for Mastitis (Nacl test). </li></ul><ul><li>Isolation of microorganisms. </li></ul><ul><li>Identification of microorganisms. </li></ul>
  5. 5. <ul><li>MATERIALS </li></ul><ul><li>Reagent requirement for media preparation. </li></ul><ul><li>Other requirements. </li></ul><ul><li>Instrument Requirement. </li></ul><ul><li>Glassware requirement. </li></ul>
  6. 6. <ul><li>METHODS </li></ul><ul><li>Collection Of Milk Samples. </li></ul><ul><li>Determination of Ph. </li></ul><ul><li>Conformational test for mastitis (NaCl) test. </li></ul><ul><li>Isolation of microorganisms. </li></ul><ul><li>Identification of microorganisms. </li></ul><ul><li>Microscopic Examination. </li></ul><ul><li>Identification of the selected culture. </li></ul><ul><li>Growth of samples on E M B Agar. </li></ul><ul><li>Growth of samples on M S A. </li></ul><ul><li>Growth of samples on P D A. </li></ul><ul><li>Catalase test. </li></ul><ul><li>Nitrate reduction test. </li></ul><ul><li>Growth at different tempratures. </li></ul>
  7. 7. <ul><li>Biochemical tests </li></ul><ul><li>IMViC : </li></ul><ul><li>The Indole Test </li></ul><ul><li>The Methyl Red and Voges-Proskauer Tests </li></ul><ul><li>The Citrate Test </li></ul><ul><li>Fermentation of different carbohydrates </li></ul>
  8. 8. <ul><li>RESULTS </li></ul><ul><li>Determination of pH </li></ul>7.02 E 6.5 D 7.12 C 7.2 B 7.14 A pH Samples
  9. 9. <ul><li>Conformational test for mastitis: </li></ul><ul><li>NaCl test </li></ul>Positive E Negative D Positive C Positive B Positive A Mastitis Samples
  10. 10. <ul><li>Isolation of microorganisms from milk sample . </li></ul><ul><li>Isolation of bacteria on Nutrient Agar Media </li></ul>White E Yellow and White D Yellow and White C White B Yellow and white A Colonies Samples
  11. 11. <ul><li>Identification of microorganisms: </li></ul>Rods Gram negative E Cocci Rods Gram positive Gram negative D Cocci Rods Gram positive Gram negative C Rods Gram negative B Cocci Rods Gram positive Gram negative A Cell morphology Gram’s staining Samples
  12. 12. <ul><li>Growth on EMB </li></ul>Metallic sheen Positive E Pink colonies Positive D Metallic sheen Positive C Metallic sheen Positive B Pink colonies Positive A Appearance Growth Samples
  13. 13. <ul><li>Growth on Mannitol salt agar </li></ul>_ Negative E Yellow colour colonies Positive D Yellow colour colonies Positive C _ Negative B Yellow colour colonies Positive A Appearance Growth Samples
  14. 14. <ul><li>Growth on Potato dextrose agar: </li></ul>Black colour Colonies Positive E _ Negative D Black colour colonies Positive C _ Negative B Black colour colonies Positive A Appearance Growth Samples
  15. 15. <ul><li>Catalase test: </li></ul>Positive E Positive D Positive C Positive B Positive A Results Samples
  16. 16. <ul><li>Growth at different temperatures </li></ul>NIL G LG E NIL G G D NIL G LG C NIL G LG B NIL G LG A 42± 0 C 37±1 0 C 27±1 0 C Samples
  17. 17. <ul><li>Biochemical tests: </li></ul><ul><li>IMViC Test: </li></ul>_ _ + _ _ _ _ + Citrate _ + + + _ _ + + VP + + _ + + + + _ MR + _ _ _ + + _ _ Indole White Yellow White Yellow White White Yellow White Colony E D C B A Sample
  18. 18. <ul><li>Fermentation of Carbohydrates: </li></ul>AG A AG A AG AG A AG Dex AG A AG A AG AG A AG Lac A A AG A A A A AG Suc AG A A A AG AG A A Glu White Yellow White Yellow White White Yellow White Colony E D C B A Sample
  19. 19. <ul><li>THANKS TO ALL </li></ul>

×