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HANIF BIN ABDUL BAHARINSTITUTE OF TROPICAL AGRICULTUREUNIVERSITI PUTRA MALAYSIASupervisory committee
A VITAMIN E GENE FROM OIL PALM:            ISOLATION OFGAMMA-TOCOPHEROL METHYLTRANSFERASE        FULL GENE STRUCTURE
INTRODUCTION &LITERATURE REVIEW
Introduction  Oil Palm Cultivation• Oil Palm is a tropical perennial monocot species in the  order Arecales of the family ...
Vitamin E in Oil Palm• Vitamin E consists         of    tocopherols    and  tocotrienols .• Vitamin E in palm oil is made ...
Main ObjectivesTo isolate full length cDNA of gama-tocopherol methyltransferase (γ-TMT)gene from oil palm.  To obtain γ-TM...
Literature Review   Vitamin E Chemical Structure                Tocopherol                Tocotrienol• Tocopherols and toc...
Vitamin E biosynthesis pathway:
Health Benefits of Vitamin E                                  Inhibit growth of Reduce blood                       cancer ...
METHODOLOGY
Overview MethodologyA. Full length cDNA Gene IsolationOil Palm             RNA             RACE techniquemesocarp         ...
B. Full length Gene Structure  Oil            DNA             PCR   GenomeWalker Palm         extractionleaves            ...
RESULTS &DISCUSSIONS
For more information, please contact me via email:hanif_biotech@yahoo.comOr Facebook:fb.me/hanif85
Acknowledgement
Hanif@MGC9
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Hanif@MGC9

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Transcript of "Hanif@MGC9"

  1. 1. HANIF BIN ABDUL BAHARINSTITUTE OF TROPICAL AGRICULTUREUNIVERSITI PUTRA MALAYSIASupervisory committee
  2. 2. A VITAMIN E GENE FROM OIL PALM: ISOLATION OFGAMMA-TOCOPHEROL METHYLTRANSFERASE FULL GENE STRUCTURE
  3. 3. INTRODUCTION &LITERATURE REVIEW
  4. 4. Introduction Oil Palm Cultivation• Oil Palm is a tropical perennial monocot species in the order Arecales of the family Aracaceae.• The African Oil Palm, Elaeis guineensis is native to West Africa, occurring between Angola and Gambia, while the American Oil Palm Elaeis oleifera is native to tropical Central America and South America. (Dransfield et al., 2005)• Oil palm was first brought to Malaya in early twentieth century as ornamental plant. It is now one of the main commodity crop in Malaysia.
  5. 5. Vitamin E in Oil Palm• Vitamin E consists of tocopherols and tocotrienols .• Vitamin E in palm oil is made up of 70% tocotrienol and 30% of tocopherol.• Palm oil is nature’s richest source of tocotrienols.• Whereas most other vegetable oils consists of only tocopherols. (Koh, 2003)
  6. 6. Main ObjectivesTo isolate full length cDNA of gama-tocopherol methyltransferase (γ-TMT)gene from oil palm. To obtain γ-TMT gene full gene structure DNA sequence. To identify single nucleotide polymorphism (SNP) in γ-TMT gene.
  7. 7. Literature Review Vitamin E Chemical Structure Tocopherol Tocotrienol• Tocopherols and tocotrienols consists of a chromanol head group with 1,2 or 3 methyl groups and an isoprenoid side chain. (Chen et al., 2006).• Tocopherols have a saturated phytyl tail, while tocotrienols have a 3-fold unsaturated side chain. (DellaPenna & Pagson,2005).• Tocopherols and tocotrienols differ only in the number and position of methyl substituents on the aromatic ring with α- having three, β- and γ- having two, and δ- tocopherol having only one. (Hunter & Cahoon, 2007).
  8. 8. Vitamin E biosynthesis pathway:
  9. 9. Health Benefits of Vitamin E Inhibit growth of Reduce blood cancer cells cholesterol Health benefits antioxidant/ neuroprotection antiaging inhibit angiogenesis
  10. 10. METHODOLOGY
  11. 11. Overview MethodologyA. Full length cDNA Gene IsolationOil Palm RNA RACE techniquemesocarp extraction Full length cDNA gene
  12. 12. B. Full length Gene Structure Oil DNA PCR GenomeWalker Palm extractionleaves Full length gene sturcture
  13. 13. RESULTS &DISCUSSIONS
  14. 14. For more information, please contact me via email:hanif_biotech@yahoo.comOr Facebook:fb.me/hanif85
  15. 15. Acknowledgement
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