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T6

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    • 1. 5 th National Symposium on Biosafety: A Rational Basis for Biocontainment Biosafety in Microbiological and Biomedical Laboratories 3 rd Edition JY Richmond & RW McKinney (eds.)
    • 2. BMBL Introduction <ul><li>1941 - Meyer and Eddie </li></ul><ul><ul><li>74 lab associated brucellosis infections in US </li></ul></ul><ul><li>1949 - Sulkin and Pike </li></ul><ul><ul><li>222 viral infections (21 fatal) </li></ul></ul><ul><ul><li>Only 27% related to known accidents </li></ul></ul>
    • 3. BMBL Introduction <ul><li>1951,1965, 1976 - Sulkin and Pike </li></ul><ul><li>Surveys for lab-associated infections </li></ul><ul><ul><li>More than 5,000 labs </li></ul></ul><ul><ul><li>Cumulative total of 3,921cases cited </li></ul></ul><ul><ul><li>Most commonly reported: </li></ul></ul><ul><ul><ul><li>Hepatitis  Brucellosis </li></ul></ul></ul><ul><ul><ul><li>Tuberculosis  Tularemia </li></ul></ul></ul><ul><ul><ul><li>Typhoid </li></ul></ul></ul><ul><ul><ul><li>Venzuelan Equine Encephalitis </li></ul></ul></ul>
    • 4. BMBL Introduction <ul><li>1951,1965, 1976 - Sulkin and Pike (cont.) </li></ul><ul><li>Surveys for lab-associated infections </li></ul><ul><ul><li>Fewer than 20% associated with known accidents </li></ul></ul><ul><ul><li>Exposure to infectious aerosols plausible (but unconfirmed) for >80% of reported cases </li></ul></ul>
    • 5. Principles of Biosafety Introduction <ul><li>Biosafety Levels 1-3 </li></ul><ul><li>Guidelines to describe combinations of: </li></ul><ul><ul><li>Laboratory Practices and Techniques </li></ul></ul><ul><ul><ul><li>Standard Practices </li></ul></ul></ul><ul><ul><ul><li>Special Practices </li></ul></ul></ul><ul><ul><li>Safety Equipment (Primary Barriers) </li></ul></ul><ul><ul><li>Laboratory Facilities (Secondary Barriers) </li></ul></ul>
    • 6. Principles of Biosafety Introduction <ul><li>Biosafety Levels 1-3 Provide </li></ul><ul><ul><li>Increasing levels of personnel and environmental protection </li></ul></ul><ul><ul><li>Guidelines for working safely in microbiological and biomedical laboratories </li></ul></ul>
    • 7. Lab Practices and Techniques Introduction <ul><ul><li>Knowledgeable supervisor </li></ul></ul><ul><ul><li>Personnel </li></ul></ul><ul><ul><ul><li>Aware of potential hazards </li></ul></ul></ul><ul><ul><ul><li>Proficient in practices/techniques </li></ul></ul></ul><ul><ul><li>Biosafety manual specific to lab </li></ul></ul>
    • 8. Safety Equipment (Primary Barriers) - Introduction <ul><ul><li>Biosafety cabinets (BSCs) [BSL-2/3] </li></ul></ul><ul><ul><li>Personal protective clothing </li></ul></ul><ul><ul><ul><li>Gloves </li></ul></ul></ul><ul><ul><ul><li>Gowns </li></ul></ul></ul><ul><ul><li>Pipetting Devices </li></ul></ul><ul><ul><li>Safety centrifuge cups and rotors </li></ul></ul><ul><ul><li>Eye and face protection </li></ul></ul><ul><ul><li>Respiratory protection [BSL-3] </li></ul></ul>
    • 9. Biosafety Level 1 Introduction <ul><li>Suitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment. </li></ul>
    • 10. Biosafety Level 1 Introduction <ul><li>Examples: </li></ul><ul><ul><li>Bacillus subtilis </li></ul></ul><ul><ul><li>Naegleria gruberi </li></ul></ul><ul><ul><li>Infectious canine hepatitis virus </li></ul></ul><ul><ul><li>E. coli </li></ul></ul>
    • 11. Biosafety Level 1 Laboratory Facilities (Secondary Barriers)
    • 12. Biosafety Level 1 Laboratory Facilities (Secondary Barriers) <ul><ul><li>Sink for handwashing </li></ul></ul><ul><ul><li>Work surfaces easily cleaned </li></ul></ul><ul><ul><li>Bench tops </li></ul></ul><ul><ul><li>Sturdy furniture </li></ul></ul><ul><ul><li>Windows fitted with flyscreens </li></ul></ul>

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