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  • 1. 5 th National Symposium on Biosafety: A Rational Basis for Biocontainment Biosafety in Microbiological and Biomedical Laboratories 3 rd Edition JY Richmond & RW McKinney (eds.)
  • 2. BMBL Introduction
    • 1941 - Meyer and Eddie
      • 74 lab associated brucellosis infections in US
    • 1949 - Sulkin and Pike
      • 222 viral infections (21 fatal)
      • Only 27% related to known accidents
  • 3. BMBL Introduction
    • 1951,1965, 1976 - Sulkin and Pike
    • Surveys for lab-associated infections
      • More than 5,000 labs
      • Cumulative total of 3,921cases cited
      • Most commonly reported:
        • Hepatitis  Brucellosis
        • Tuberculosis  Tularemia
        • Typhoid
        • Venzuelan Equine Encephalitis
  • 4. BMBL Introduction
    • 1951,1965, 1976 - Sulkin and Pike (cont.)
    • Surveys for lab-associated infections
      • Fewer than 20% associated with known accidents
      • Exposure to infectious aerosols plausible (but unconfirmed) for >80% of reported cases
  • 5. Principles of Biosafety Introduction
    • Biosafety Levels 1-3
    • Guidelines to describe combinations of:
      • Laboratory Practices and Techniques
        • Standard Practices
        • Special Practices
      • Safety Equipment (Primary Barriers)
      • Laboratory Facilities (Secondary Barriers)
  • 6. Principles of Biosafety Introduction
    • Biosafety Levels 1-3 Provide
      • Increasing levels of personnel and environmental protection
      • Guidelines for working safely in microbiological and biomedical laboratories
  • 7. Lab Practices and Techniques Introduction
      • Knowledgeable supervisor
      • Personnel
        • Aware of potential hazards
        • Proficient in practices/techniques
      • Biosafety manual specific to lab
  • 8. Safety Equipment (Primary Barriers) - Introduction
      • Biosafety cabinets (BSCs) [BSL-2/3]
      • Personal protective clothing
        • Gloves
        • Gowns
      • Pipetting Devices
      • Safety centrifuge cups and rotors
      • Eye and face protection
      • Respiratory protection [BSL-3]
  • 9. Biosafety Level 1 Introduction
    • Suitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.
  • 10. Biosafety Level 1 Introduction
    • Examples:
      • Bacillus subtilis
      • Naegleria gruberi
      • Infectious canine hepatitis virus
      • E. coli
  • 11. Biosafety Level 1 Laboratory Facilities (Secondary Barriers)
  • 12. Biosafety Level 1 Laboratory Facilities (Secondary Barriers)
      • Sink for handwashing
      • Work surfaces easily cleaned
      • Bench tops
      • Sturdy furniture
      • Windows fitted with flyscreens