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T6

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  • Transcript

    • 1. 5 th National Symposium on Biosafety: A Rational Basis for Biocontainment Biosafety in Microbiological and Biomedical Laboratories 3 rd Edition JY Richmond & RW McKinney (eds.)
    • 2. BMBL Introduction
      • 1941 - Meyer and Eddie
        • 74 lab associated brucellosis infections in US
      • 1949 - Sulkin and Pike
        • 222 viral infections (21 fatal)
        • Only 27% related to known accidents
    • 3. BMBL Introduction
      • 1951,1965, 1976 - Sulkin and Pike
      • Surveys for lab-associated infections
        • More than 5,000 labs
        • Cumulative total of 3,921cases cited
        • Most commonly reported:
          • Hepatitis  Brucellosis
          • Tuberculosis  Tularemia
          • Typhoid
          • Venzuelan Equine Encephalitis
    • 4. BMBL Introduction
      • 1951,1965, 1976 - Sulkin and Pike (cont.)
      • Surveys for lab-associated infections
        • Fewer than 20% associated with known accidents
        • Exposure to infectious aerosols plausible (but unconfirmed) for >80% of reported cases
    • 5. Principles of Biosafety Introduction
      • Biosafety Levels 1-3
      • Guidelines to describe combinations of:
        • Laboratory Practices and Techniques
          • Standard Practices
          • Special Practices
        • Safety Equipment (Primary Barriers)
        • Laboratory Facilities (Secondary Barriers)
    • 6. Principles of Biosafety Introduction
      • Biosafety Levels 1-3 Provide
        • Increasing levels of personnel and environmental protection
        • Guidelines for working safely in microbiological and biomedical laboratories
    • 7. Lab Practices and Techniques Introduction
        • Knowledgeable supervisor
        • Personnel
          • Aware of potential hazards
          • Proficient in practices/techniques
        • Biosafety manual specific to lab
    • 8. Safety Equipment (Primary Barriers) - Introduction
        • Biosafety cabinets (BSCs) [BSL-2/3]
        • Personal protective clothing
          • Gloves
          • Gowns
        • Pipetting Devices
        • Safety centrifuge cups and rotors
        • Eye and face protection
        • Respiratory protection [BSL-3]
    • 9. Biosafety Level 1 Introduction
      • Suitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.
    • 10. Biosafety Level 1 Introduction
      • Examples:
        • Bacillus subtilis
        • Naegleria gruberi
        • Infectious canine hepatitis virus
        • E. coli
    • 11. Biosafety Level 1 Laboratory Facilities (Secondary Barriers)
    • 12. Biosafety Level 1 Laboratory Facilities (Secondary Barriers)
        • Sink for handwashing
        • Work surfaces easily cleaned
        • Bench tops
        • Sturdy furniture
        • Windows fitted with flyscreens