Proteomics & Metabolomics
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Proteomics & Metabolomics

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Proteomics & Metabolomics Proteomics & Metabolomics Presentation Transcript

  • Proteomics and Metabolomics Shared Resource (PMSR) Stephen Byers, PhD, Co-Director Al Fornace, MD, Co-Director Amrita Cheema, PhD, Metabolomics [email_address] Rado Goldman, Proteomics [email_address]
  • Proteomics: An Integrated Approach Gel Based Workflow LC Based Workflow Integrated Proteomics
  • 2D Gel Workflow
    • Applications:
    • Multiplexing of gels and visualization of differentially expressed spots between the sample and the control on the same gel.
    • Differential Protein analysis: using the De Cyder Software.
    • Improved spot detection: due to high sensitivity of the fluorescent labeling.
    • Imager: used for other fluorescent imaging applications.
    DIGE Imager 2D DIGE ECL Plex Western Blot View slide
  • Spot Picker
    • Robotic spot picker:
    • picks spots from 2D gels with increased accuracy
    • efficiency based on the protein pick list generated by the image analysis software.
    View slide
  • MALDI TOF-TOF Mass Spectrometers
    • Applications:
    • Identification of Proteins from complex
    • biological samples using MASCOT.
    • Supports LC MALDI workflows for isobaric
    • labeling of proteins followed by identification.
    • Serum Biomarker studies.
    • In-Silico digest workflows for the analysis of post translational modifications.
    Nano LC coupled with Probotic Spotter 4800 Proteomics Analyzer
  • Nano Acquity UPLC- QSTAR ELITE LCMS/MS system
    • Optimized for high throughput
    • ‘ Shot gun’ proteomics workflows
    • in conjunction with the online nano flow TEMPO MDLC system.
    • High Speed data acquisition, database searching and processing
    • Software.
    • Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling
  • MALDI TOF-TOF Mass Spectrometers
    • Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling.
    • Detection of Post translational modifications.
    • Serum Profiling studies made easy with marker view softwares for Principle component analysis.
    • Metabolomics and metabolite detection.
    • Detection and Identification of low abundance proteins due to high sensitivity and mass accuracy.
  • What Responses are Possible?
  • UPLC-TOFMS
    • Applications:
    • Small molecule profiling
    • Small molecule quantization
  • Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.