Proteomics & Metabolomics

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Proteomics & Metabolomics

  1. 1. Proteomics and Metabolomics Shared Resource (PMSR) Stephen Byers, PhD, Co-Director Al Fornace, MD, Co-Director Amrita Cheema, PhD, Metabolomics [email_address] Rado Goldman, Proteomics [email_address]
  2. 2. Proteomics: An Integrated Approach Gel Based Workflow LC Based Workflow Integrated Proteomics
  3. 3. 2D Gel Workflow <ul><li>Applications: </li></ul><ul><li>Multiplexing of gels and visualization of differentially expressed spots between the sample and the control on the same gel. </li></ul><ul><li>Differential Protein analysis: using the De Cyder Software. </li></ul><ul><li>Improved spot detection: due to high sensitivity of the fluorescent labeling. </li></ul><ul><li>Imager: used for other fluorescent imaging applications. </li></ul>DIGE Imager 2D DIGE ECL Plex Western Blot
  4. 4. Spot Picker <ul><li>Robotic spot picker: </li></ul><ul><li>picks spots from 2D gels with increased accuracy </li></ul><ul><li>efficiency based on the protein pick list generated by the image analysis software. </li></ul>
  5. 5. MALDI TOF-TOF Mass Spectrometers <ul><li>Applications: </li></ul><ul><li>Identification of Proteins from complex </li></ul><ul><li>biological samples using MASCOT. </li></ul><ul><li>Supports LC MALDI workflows for isobaric </li></ul><ul><li>labeling of proteins followed by identification. </li></ul><ul><li>Serum Biomarker studies. </li></ul><ul><li>In-Silico digest workflows for the analysis of post translational modifications. </li></ul>Nano LC coupled with Probotic Spotter 4800 Proteomics Analyzer
  6. 6. Nano Acquity UPLC- QSTAR ELITE LCMS/MS system <ul><li>Optimized for high throughput </li></ul><ul><li>‘ Shot gun’ proteomics workflows </li></ul><ul><li>in conjunction with the online nano flow TEMPO MDLC system. </li></ul><ul><li>High Speed data acquisition, database searching and processing </li></ul><ul><li>Software. </li></ul><ul><li>Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling </li></ul>
  7. 7. MALDI TOF-TOF Mass Spectrometers <ul><li>Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling. </li></ul><ul><li>Detection of Post translational modifications. </li></ul><ul><li>Serum Profiling studies made easy with marker view softwares for Principle component analysis. </li></ul><ul><li>Metabolomics and metabolite detection. </li></ul><ul><li>Detection and Identification of low abundance proteins due to high sensitivity and mass accuracy. </li></ul>
  8. 8. What Responses are Possible?
  9. 9. UPLC-TOFMS <ul><li>Applications: </li></ul><ul><li>Small molecule profiling </li></ul><ul><li>Small molecule quantization </li></ul>
  10. 10. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.

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