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Proteomics & Metabolomics

Proteomics & Metabolomics






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    Proteomics & Metabolomics Proteomics & Metabolomics Presentation Transcript

    • Proteomics and Metabolomics Shared Resource (PMSR) Stephen Byers, PhD, Co-Director Al Fornace, MD, Co-Director Amrita Cheema, PhD, Metabolomics [email_address] Rado Goldman, Proteomics [email_address]
    • Proteomics: An Integrated Approach Gel Based Workflow LC Based Workflow Integrated Proteomics
    • 2D Gel Workflow
      • Applications:
      • Multiplexing of gels and visualization of differentially expressed spots between the sample and the control on the same gel.
      • Differential Protein analysis: using the De Cyder Software.
      • Improved spot detection: due to high sensitivity of the fluorescent labeling.
      • Imager: used for other fluorescent imaging applications.
      DIGE Imager 2D DIGE ECL Plex Western Blot
    • Spot Picker
      • Robotic spot picker:
      • picks spots from 2D gels with increased accuracy
      • efficiency based on the protein pick list generated by the image analysis software.
    • MALDI TOF-TOF Mass Spectrometers
      • Applications:
      • Identification of Proteins from complex
      • biological samples using MASCOT.
      • Supports LC MALDI workflows for isobaric
      • labeling of proteins followed by identification.
      • Serum Biomarker studies.
      • In-Silico digest workflows for the analysis of post translational modifications.
      Nano LC coupled with Probotic Spotter 4800 Proteomics Analyzer
    • Nano Acquity UPLC- QSTAR ELITE LCMS/MS system
      • Optimized for high throughput
      • ‘ Shot gun’ proteomics workflows
      • in conjunction with the online nano flow TEMPO MDLC system.
      • High Speed data acquisition, database searching and processing
      • Software.
      • Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling
    • MALDI TOF-TOF Mass Spectrometers
      • Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling.
      • Detection of Post translational modifications.
      • Serum Profiling studies made easy with marker view softwares for Principle component analysis.
      • Metabolomics and metabolite detection.
      • Detection and Identification of low abundance proteins due to high sensitivity and mass accuracy.
    • What Responses are Possible?
      • Applications:
      • Small molecule profiling
      • Small molecule quantization
    • Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.