http://www.genomecenter.howard.edu/ National Human Genome Center Molecular Genetics Georgia M. Dunston, PhD Mezbah U. Faruque, MD, PhD Director Associate Director Yuanxiu Chen, MD, PhD Paulette Furbert-Harris, PhD CTSA Laboratory Director Core Laboratory Director Muneer Abbas, PhD Tshela Mason, MS Laboratory Manager Operations Supervisor Luisel Ricks-Santi, PhD Biorepository Coordinator Contact Mezbah Faruque at 202-806-4780 or e-mail at firstname.lastname@example.org
well as from formalin fixed paraffin embedded tissue.
Quantification of the DNA and RNA using
spectrophotometry and NanoDrop® .
Further quality control of DNA by restriction digestion
http://www.genomecenter.howard.edu/ Three PSQ™HS 96 sequencers with built-in robotic mechanism for transfer of ten 96-well plates for high throughput SNP genotyping. Platform compatible primers are designed using PSQ assay design software. Current throughput for the pyrosequencers in our labs exceeds 12,000 genotypes per day. High-throughput pyrosequencing for SNP genotyping
http://www.genomecenter.howard.edu/ Performed on ABI 3100 automated DNA sequencers using big dye terminator chemistry . Custom primer designing for amplification and sequencing of up to 500 bp of bi-directional reads. Direct DNA Sequencing
http://www.genomecenter.howard.edu/ HLA genotyping HLA genotyping after reverse PCR-SSO using Luminex Technology that offers a microspehere based assay with multiplexed data acquisition and analyses. High resolution sequence based typing of HLA-B, HLA-DRB1 and HLA-DQB1 to identify new HLA alleles as well as to assign allele calls by comparing sequences with reference databases.
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01 In your publications, remember to mention support provided from the GHUCCTS.