Flow Cytometry/Cell Sorting Shared Resource (FCSR) Karen Creswell, PhD,  Director [email_address] 202-687-2233
FCSR – Apoptosis Assays <ul><li>Sub G1 peak </li></ul><ul><li>Annexin V (membrane flip-flop) </li></ul><ul><li>TUNEL assay...
FCSR:  Stem Cell Assays <ul><li>Immunophenotyping </li></ul><ul><li>Aldefluor analysis </li></ul><ul><li>Side population (...
FCSR:  Other Applications <ul><li>Cell Cycle </li></ul><ul><li>Immunophenotyping </li></ul><ul><li>Cytoxicity Assays </li>...
FCSR:  Cell Sorting <ul><li>Separation of cell populations  </li></ul><ul><ul><li>Can sort up to 4 populations simultaneou...
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies...
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Flow Cytometry/Cell Sorting

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Flow Cytometry/Cell Sorting

  1. 1. Flow Cytometry/Cell Sorting Shared Resource (FCSR) Karen Creswell, PhD, Director [email_address] 202-687-2233
  2. 2. FCSR – Apoptosis Assays <ul><li>Sub G1 peak </li></ul><ul><li>Annexin V (membrane flip-flop) </li></ul><ul><li>TUNEL assay (DNA strand breaks) </li></ul><ul><li>JC-1 staining (mitochondrial membrane potential) </li></ul><ul><li>Caspase and caspase activity assays </li></ul>
  3. 3. FCSR: Stem Cell Assays <ul><li>Immunophenotyping </li></ul><ul><li>Aldefluor analysis </li></ul><ul><li>Side population (SP) detection </li></ul><ul><li>Any of these methods or a combination </li></ul><ul><li>of methods can be utilized to analyze </li></ul><ul><li>and sort stem cells </li></ul>
  4. 4. FCSR: Other Applications <ul><li>Cell Cycle </li></ul><ul><li>Immunophenotyping </li></ul><ul><li>Cytoxicity Assays </li></ul><ul><li>Measurement of ROS </li></ul><ul><li>Phagocytosis Assays </li></ul><ul><li>Intracellular cytokine analysis </li></ul><ul><li>Cell signaling antigen detection </li></ul><ul><li>Fluorescent protein analysis (GFP, CFP, YFP, mCherry, etc.) </li></ul>
  5. 5. FCSR: Cell Sorting <ul><li>Separation of cell populations </li></ul><ul><ul><li>Can sort up to 4 populations simultaneously </li></ul></ul><ul><ul><li>Can sort into 6-, 24, or 96-well plates </li></ul></ul>
  6. 6. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.
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